SP-LL-37, human antimicrobial peptide, enhances disease resistance in transgenic rice

Lee, In Hye; Jung, Yu‐Jin; Cho, Yong Gu; Nou, Ill Sup; Huq, Md. Amdadul; Nogoy, Franz Marielle; Kang, Kwon-Kyoo

doi:10.1371/journal.pone.0172936

Cited by 13 publications

(13 citation statements)

References 23 publications

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“…Japonica cv. Dongjinbyeo), and as a result transgenic plants exhibited enhanced resistance against various plant pathogenic organisms. However, expression of LL‐37 in transgenic plants for molecular farming and subsequent analysis of the heterologous product have not been reported.…”

Section: Introductionmentioning

confidence: 99%

Molecular Farming in Barley: Development of a Novel Production Platform to Produce Human Antimicrobial Peptide LL-37

et al. 2018

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The peptide LL-37, a component of the human innate immune system, represents a promising drug candidate. In particular, the development of low-cost production platform technology is a critical bottleneck in its use in medicine. In the present study, a viable approach for the LL-37 production in transgenic barley is developed. First, comparative analyses of the effects of different fused peptide epitope tags applicable for accumulation and purification on LL-37 production yield are performed using transient expression in tobacco leaves. Following the selection of the most yielding fusion peptide strategies, eight different constructs for the expression of codon optimized chimeric LL-37 genes in transgenic barley plants are created. The expression of individual constructs is driven either by an endosperm-specific promoter of the barley B1 hordein gene or by the maize ubiquitin promoter. The transgenes are stably integrated into the barley genome and inherited in the subsequent generation. All transgenic lines show normal phenotypes and are fertile. LL-37 accumulated in the barley seeds up to 0.55 mg per 1 kg of grain. The fused epitope tags are cleaved off by the use of enterokinase. Furthermore, in planta produced LL-37 including the fused versions is biologically active.

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Section: Introductionmentioning

confidence: 99%

Molecular Farming in Barley: Development of a Novel Production Platform to Produce Human Antimicrobial Peptide LL-37

et al. 2018

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“…Human cathelicidin LL-37 has been used widely for developing many transgenic crops in the past few years (Jung, 2013;Jung et al, 2012;Lee et al, 2017). It was found that LL-37 is susceptible to salt toxicity resulting from deactivation in highly alkaline medium.…”

Section: Discussionmentioning

confidence: 99%

“…Although slight variations in plant–microbe composition in root and leaf microbiota have been reported in previous studies on tobacco plants (Weinhold, Dorcheh, Li, Rameshkumar, & Baldwin, ), existing plant pathogens have already developed a high resistance to the endogenous AMPs of plants. In surveys of other types of peptides, the microbial peptides of animal origin are found to be less toxic, highly efficient and quicker in inhibiting pathogens (Lee et al, ). Therefore, integrating animal‐origin exogenous AMPs into plant genomes presents an excellent way to protect plants without affecting their natural plant microbial composition and development.…”

Section: Introductionmentioning

confidence: 99%

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Effect of WAM‐1 transgenic Arabidopsis thaliana on the development and gut microbial composition of diamondback moth (Plutella xylostella) larvae (Lepidoptera: Plutellidae)

Arachchige

Pei

et al. 2019

J Applied Entomology

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WAM‐1 is a cathelicidin antimicrobial peptide found on the Macropus eugenii (Tammar wallaby) genome. It possesses antibiotic activity against a broad spectrum of bacteria and fungi. In order to investigate the impact of this WAM‐1 antimicrobial peptide on development and midgut microbial composition of Plutella xylostella (DBM) larvae, the WAM‐1 was introduced into Arabidopsis thaliana using the Agrobacterium‐mediated floral‐dip transformation method. WAM‐1 expression was confirmed by reverse transcriptase PCR analysis. The larval growth and developmental variations and midgut microbial compositions were analysed after DBM larvae had fed on transgenic plants. Transgenic plant‐fed DBM larvae showed significantly higher mortality, a prolonged developmental period and less weight gain. Enterobacteriaceae was the highest dominating family in all samples including transgenic and non‐transgenic plants fed larvae. However, its abundance was substantially lower in the WAM‐1 Arabidopsis‐fed larvae. Furthermore, Moraxellaceae and Bacillaceae were the next dominating families in the WAM‐1 transgenic Arabidopsis‐fed larvae whereas Enterococcaceae and Xanthomonadaceae were dominant in the non‐transgenic Arabidopsis‐fed larvae. Abundance of Pseudomonadaceae was also relatively higher in WAM‐1 transgenic‐fed larvae whereas it was much lower in other samples. Therefore, our findings suggest that WAM‐1 has an impact on gut microbial composition but a minor effect on larval development.

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“…Bearing in mind the application of antimicrobial peptides and proteins, genetically modified (GM) plants can be the platform of choice for their expression. Research shows that it is possible to successfully express a wide variety of antimicrobial proteins in plants, starting from those which can protect crops against bacteria (Lee et al 2017), but also those which can be subsequently used as biopharmaceuticals with or without a later purification step (Paškevičius et al 2017;Company et al 2014). Recombinant protein production in edible plants or those used as an animal feed gives a possibility of cutting the costs of product extraction and purification.…”

Section: Introductionmentioning

confidence: 99%

Production of recombinant colicin M in Nicotiana tabacum plants and its antimicrobial activity

Łojewska

Sakowicz

Kowalczyk

et al. 2019

Plant Biotechnol Rep

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Antibiotic-resistant microorganisms causing a life-threatening infection pose a serious challenge for modern science. The rapidly growing number of incidents for which the use of standard antibiotics is ineffective forces us to develop new alternative methods of killing microorganisms. Antimicrobial proteins and peptides (AMPs) can be promising candidates to solve this problem. Colicin-M is one of the representatives of this group and is naturally produced by Escherichia coli acting on other closely related bacterial strains by disrupting their outer cell membrane. This bacteriocin has huge potential as a potent antimicrobial agent, especially, since it was recognized by the FDA as safe. In this work, we present the expression of colicin M in model transgenic Nicotiana tabacum L. plants. We demonstrate that purified colicin retains its antibacterial activity against the control Escherichia coli strains and clinical isolates of Escherichia coli and Klebsiella pneumoniae. Our results also show that plant-derived ColM is not toxic for L929 and HeLa cell line, which allows us to suppose that plant-based expression could be an alternative production method of such important proteins.

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SP-LL-37, human antimicrobial peptide, enhances disease resistance in transgenic rice

Cited by 13 publications

References 23 publications

Molecular Farming in Barley: Development of a Novel Production Platform to Produce Human Antimicrobial Peptide LL-37

Molecular Farming in Barley: Development of a Novel Production Platform to Produce Human Antimicrobial Peptide LL-37

Effect of WAM‐1 transgenic Arabidopsis thaliana on the development and gut microbial composition of diamondback moth (Plutella xylostella) larvae (Lepidoptera: Plutellidae)

Production of recombinant colicin M in Nicotiana tabacum plants and its antimicrobial activity

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