Soybean spermidine concentration: Genetic and environmental variation of a potential ‘anti-aging’ constituent

Sagara, Tatsuya; Fiechter, Gregor; Pachner, Martin; Mayer, Hans; Vollmann, J.

doi:10.1016/j.jfca.2016.11.008

Cited by 16 publications

(20 citation statements)

References 36 publications

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“…Chromatographic reversed-phase separation of fAA and amines after AQC derivatization has been proposed for coffee [12] and other matrices, using different modes of detection, mostly UV/PDA or FLR. The proposed methodology derives from the experience of the quantification of fAA and amines in soy [27] and dairy products [28] employing similar extraction and derivatization procedures. Using a serial configuration of UV and FLR detection on an UHPLC platform merges the benefits of both modes of detection ( Fig.…”

Section: Implementation and Validation Of The Uhplc-uv-flr Methodsmentioning

confidence: 99%

“…Preparation of nutritional supplements (Suppl.) was derived from a recently published method [27] and was conducted as follows: solid samples (Suppl.) were weighed in (5.0 g) with an analytical balance (Sartorius, Goettingen, Germany…”

Section: Preparation Of Nutritional Supplementsmentioning

confidence: 99%

“…Chromatographic separation and analysis of fAA-or MA-, DA-and PA-AQC derivatives was performed on a Waters Acquity™ Ultra Performance LC (UPLC™) using a serial ultraviolet (UV) and fluorescence (FLR) detection set-up, combining previously published separate UV and FLR set-ups [27,28], consisting of a Acquity™ tunable ultraviolet (TUV) and Acquity™ fluorescence (FLR) detector. Serial instrument set-up was achieved by connecting the FLR detector cell inlet with the TUV detector cell outlet, using PEEK tubing (waters).…”

Section: Uhplc Conditionsmentioning

confidence: 99%

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Green coffee derived supplements and infusions as a source of polyamines and free amino acids

Macheiner

Schmidt

Mayer

2020

Eur Food Res Technol

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This work reports on monoamines (MA), diamines (DA) and polyamines (PA) as well as free amino acids (fAA) in nutritional supplements and infusions derived from green coffee beans. Samples were investigated using a ultra-high performance liquid chromatography UV/FLR method, which was validated regarding specificity, linearity, range, precision, accuracy and limits of detection and quantification. Nutritional supplements based on green coffee beans showed large amounts of total amines ranging from 1090 to 2593 mg/kg, with exceptional high levels of spermidine up to 724 mg/kg, and a content of fAA from 4004 to 12,389 mg/kg. Infusions brewed from green coffee beans showed much lower contents of amines (14–17 mg/L) and fAA (78–100 mg/L). However, if the customary edible portion was considered, infusions from green coffee were a mainly better source for MA and PA as well for fAA, compared to nutritional supplements. Tryptamine and tyramine were predominant as MA putrescine as DA, and spermidine as PA, respectively. Graphic abstract

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Section: Implementation and Validation Of The Uhplc-uv-flr Methodsmentioning

confidence: 99%

Section: Preparation Of Nutritional Supplementsmentioning

confidence: 99%

Section: Uhplc Conditionsmentioning

confidence: 99%

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Green coffee derived supplements and infusions as a source of polyamines and free amino acids

Macheiner

Schmidt

Mayer

2020

Eur Food Res Technol

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“…The discovery of cell autophagy, which received the 2016 Nobel Prize in Physiology or Medicine, has extended human life [1], and our daily living is associated with numerous functional food ingredients that regulate cell autophagy levels [2]. For example, spermidine, which is widespread in soybeans, matured cheeses, and whole grains, plays roles in various life activities, and affects cell growth [3,4], demonstrating potential applications in the biological field [5]. However, spermidine can accumulate in contaminated aquatic and meat products, and pose a strong subacute toxicity when present in high amounts [6].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of a Molecularly Imprinted Polymer of Spermidine and the Exploration of Its Molecular Recognition Properties

2018

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Spermidine is a functional ingredient that can extend the lifespan of many foods and indicate meat safety. However, its synthesis and enrichment is expensive and complex. To develop an effective separation material that can offer highly selective recognition of spermidine, we first applied non-covalent molecular imprinting technology using methacrylic acid as a functional monomer, azobisisobutyronitrile as an initiator, and ethylene glycol dimethacrylate as a cross-linker. The adsorption properties of the polymers were analyzed using the Scatchard equation, the Lagergren kinetic equation, and the static distribution coefficient. The optimal polymerization molar ratio of the template molecule spermidine to the functional monomer was 1:4, the maximum adsorption amount was 97.75 μmol/g, and the adsorption equilibrium time was 300 min. The selective experiment showed that the interfering substances tyramine and histamine had selectivity factor α values of 2.01 and 1.78, respectively, indicating that the prepared polymer had good spermidine recognition ability. The density function theory calculations showed that the hydrogen bond strength, steric effect, and product energy caused adsorption and separation differences among the different imprinted polymer complexes.

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“…Bean seeds contain polyamines such as SPD, spermine, putrescine and cadaverine. In particular, the SPD level of the soybean is considerably higher than that of common cereals, vegetables, or animal food products [ 5 ]. This might be due to the high level of arginine, a major precursor of polyamine biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

Rapid Evaluation of Spermidine from 12 Bean Cultivars by Direct Real-Time Mass Spectrometry Analysis

Yuan

et al. 2018

Molecules

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The routine spermidine (SPD) detection method is time-consuming and laborious due to the lengthy chromatographic separation and/or tedious sample derivatization pretreatment. In this study, direct analysis in real-time ionization mode coupled with mass spectrometry (DART-MS) was developed to rapidly determine the SPD content of 12 bean cultivars. The results were compared in detail with those of the classical UHPLC-ESI-QTOF method. After conducting a series of optimizations, a simple sample extraction procedure employing 80% aqueous methanol, was followed by determination of sample extracts directly without any chromatographic separation or prior derivatization. The validated method showed excellent performance with low limits of detection (LOD of 0.025 mg·kg−1) and good recovery rates (102.79–148.44%). The investigation highlighted that the DART-MS method (~1.3 min per three samples) could be used as a high-throughput alternative to the classic UHPLC-ESI-QTOF method (~15 min per three samples).

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Soybean spermidine concentration: Genetic and environmental variation of a potential ‘anti-aging’ constituent

Cited by 16 publications

References 36 publications

Green coffee derived supplements and infusions as a source of polyamines and free amino acids

Green coffee derived supplements and infusions as a source of polyamines and free amino acids

Synthesis and Characterization of a Molecularly Imprinted Polymer of Spermidine and the Exploration of Its Molecular Recognition Properties

Rapid Evaluation of Spermidine from 12 Bean Cultivars by Direct Real-Time Mass Spectrometry Analysis

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