Soybean seed coat chitinase as a defense protein against the stored product pest <i>Callosobruchus maculatus</i>

Silva, Nadia Cm; Conceição, Jamile G.; Ventury, Kayan Eudorico; Sá, Leonardo Fr De; Oliveira, Eduardo A. G.; Santos, Izabela S.; Gomes, Valdirene Moreira; Costa, Monique Nunes da; Ferreira, André T.S.; Perales, Jonás; Xavier‐Filho, J.; Fernandes, Kátia Valevski Sales; Oliveira, Antônia Elenir Amâncio

doi:10.1002/ps.4832

Cited by 14 publications

(13 citation statements)

References 32 publications

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“…Chitinase has a role in plant defense, and the overexpression of chitinase genes in pepper, and other transgenic plants enhanced 2011) demonstrated that increased chitinase activity was correlated with reduced numbers of aphids, which suggests a role for chitinase in defense against both insects, and pathogens [39]. Chitinase occurs in various plant tissues, including leaves, fruits, seeds, and roots; it is regarded as a defense-related enzyme that confers some protection against fungi, and pests [40].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling in rice reveals a positive role for OsNCED3 in defense against the brown planthopper, Nilaparvata lugens

Sun

Liu

et al. 2022

BMC Genomics

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Abstract9-cis-epoxycarotenoid dioxygenase (NCED) is the rate-limiting enzyme for abscisic acid (ABA) biosynthesis in higher plants. In rice, OsNCED3 was shown to promote ABA synthesis, and improve abiotic stress tolerance, but the function of OsNCED3 in regulating rice defense against the brown planthopper (Nilaparvata lugens; BPH) has been unclear. In this study, several parameters were used to assess rice resistance to BPH, including the average injury level, the functional plant loss index, and electrical penetration graph analysis. Rice lines overexpressing OsNCED3 (OE) were more resistant to BPH than the wild-type cv. Zhonghua11 (WT). Transcriptome analysis was performed on WT, OE, and a RNAi transgenic line silenced for OsNCED3; these three lines were either infested or non-infested with BPH. Seventeen RNA libraries were compared, and most of the differentially expressed genes (DEGs) were upregulated. The number of DEGs in the RNAi line infested with BPH was significantly higher than the OE, and WT lines, and many DEGs were related to the stress response, and biosynthesis of jasmonic acid. This study shows that overexpression of OsNCED3 in rice improves resistance to BPH, and has potential merit in rice breeding programs.

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Section: Discussionmentioning

confidence: 99%

Transcriptome profiling in rice reveals a positive role for OsNCED3 in defense against the brown planthopper, Nilaparvata lugens

Sun

Liu

et al. 2022

BMC Genomics

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“…The soybean seed coat contains chitinases, which act as a defense protein against C. maculatus. 47 Therefore, the presence of such toxic proteins in the seed coat of cv Nekador also may cause reduced oviposition rate of the weevils on it. Further studies will be necessary to substantiate this idea.…”

Section: Discussionmentioning

confidence: 99%

“…color, texture and size) between susceptible (EPACE 10) and resistant (IT81D‐1045) V. unguiculata cultivars, no significant difference was exhibited in the oviposition preference of C. maculatus on these cultivars. The soybean seed coat contains chitinases, which act as a defense protein against C. maculatus 47 . Therefore, the presence of such toxic proteins in the seed coat of cv Nekador also may cause reduced oviposition rate of the weevils on it.…”

Section: Discussionmentioning

confidence: 99%

Oviposition preference and life‐history parameters of Callosobruchus maculatus (Coleoptera: Chrysomelidae) on different soybean (Glycine max) cultivars

Naseri

Ebadollahi

Hamzavi

2022

Pest Management Science

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BACKGROUND Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae) is one of the most destructive pests of stored legume seeds in many regions of the world. Evaluation of varietal resistance of host seeds to C. maculatus infestation can be useful in preventing severe damage. We studied the susceptibility and resistance of eight conventional soybean cultivars (Caspian, Kosar, L17, Nekador, Sari, Tapour, Williams and Zane) based on the oviposition preference (choice and no‐choice tests) and population growth parameters of the pest. RESULTS Compared with other cultivars tested, oviposition preference of C. maculatus was the lowest on Nekador. The developmental time of C. maculatus differed from 40.34 days on Nekador to 48.35 days on L17, and was negatively correlated with the seed protein content. Other performance measures of C. maculatus did not match the oviposition preference. Immature survival and female longevity were highest on Caspian. The intrinsic rate of increase (rm) was higher on Tapour, Nekador and Caspian than on Zane, Williams, and Kosar. A positive correlation was observed between the rm value of the pest and moisture content of the cultivars. Cluster analysis showed that cvs Nekador, Sari, Tapour, and Caspian were relatively susceptible, and cvs Kosar, Zane, Williams and L17 were partially resistant to this pest. CONCLUSION Protein and moisture contents of soybean cultivars play an important role in the development and population growth of C. maculatus, and these traits could be used to develop safe and effective management strategies for this pest on stored soybean seeds. © 2022 Society of Chemical Industry.

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“…Recently, soybean seed coat chitinase was shown to be highly toxic to C . maculatus 30 . Thus, the genes for LecRK and chitinase can be considered candidate genes at the QTL qVmunBr6 .…”

Section: Discussionmentioning

confidence: 99%

Development of an SNP-based high-density linkage map and QTL analysis for bruchid (Callosobruchus maculatus F.) resistance in black gram (Vigna mungo (L.) Hepper)

Somta

Chen

Yundaeng

et al. 2019

Sci Rep

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Black gram ( Vigna mungo var. mungo ) is an important pulse crop in Asia. The cowpea weevil ( Callosobruchus maculatus ) is a stored-seed insect pest (seed weevil/bruchid) that causes serious postharvest losses in pulse crops, including black gram. In this study, we constructed a high-density linkage map for black gram and identified quantitative trait loci (QTLs) for C . maculatus resistance. A recombinant inbred line (RIL) population of 150 lines from a cross between BC48 [cultivated black gram (var. mungo ); bruchid-susceptible] and TC2210 [wild black gram (var. silvestris ); bruchid-resistant] were used to construct a linkage map of 3,675 SNP markers from specific-locus amplified fragment sequencing. The map comprised 11 linkage groups spanning 1,588.7 cM with an average distance between adjacent markers of 0.57 cM. Seeds of the RIL population grown in 2016 and 2017 were evaluated for C . maculatus resistance through two traits; the percentage of damaged seeds (PDS) and infestation severity progress (AUDPS). Inclusive composite interval mapping identified three QTLs each for PDS and AUDPS. Two QTLs, qVmunBr6 .1 and qVmunBr6 .2, mapped about 10 cM apart on linkage group 6 were common between PDS and AUDPS. Comparative genome analysis revealed that qVmunBr6 . 1 and qVmunBr6 . 2 are new loci for C . maculatus resistance in Vigna species and that genes encoding a lectin receptor kinase and chitinase are candidates for qVmunBr6 . 2 . The high-density linkage map constructed and QTLs for bruchid resistance identified in this study will be useful for molecular breeding of black gram.

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Soybean seed coat chitinase as a defense protein against the stored product pest Callosobruchus maculatus

Abstract: Seed coat chitinase is an important seed defense molecule not only in the cotyledons but also in seed coats, acting as part of the array of defense mechanisms against Callosobruchus maculatus. © 2017 Society of Chemical Industry.

Cited by 14 publications

References 32 publications

Transcriptome profiling in rice reveals a positive role for OsNCED3 in defense against the brown planthopper, Nilaparvata lugens

Transcriptome profiling in rice reveals a positive role for OsNCED3 in defense against the brown planthopper, Nilaparvata lugens

Oviposition preference and life‐history parameters of Callosobruchus maculatus (Coleoptera: Chrysomelidae) on different soybean (Glycine max) cultivars

Development of an SNP-based high-density linkage map and QTL analysis for bruchid (Callosobruchus maculatus F.) resistance in black gram (Vigna mungo (L.) Hepper)

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