Soybean peroxidase immobilized onto silica-coated superparamagnetic iron oxide nanoparticles: Effect of silica layer on the enzymatic activity

Donadelli, Jorge A.; Einschlag, Fernando S. García; Laurenti, Enzo; Magnacca, Giuliana; Carlos, Luciano

doi:10.1016/j.colsurfb.2017.11.043

Cited by 38 publications

(16 citation statements)

References 36 publications

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“…This enzyme is quite similar in structure and properties to the well-known horseradish peroxidase (HRP), adopted in a wide range of applications such as bioremediation and wastewater treatment, biocatalysis, diagnostic tests, therapeutics, and biosensors (Lopes et al, 2014 ; Krainer and Glieder, 2015 ). Respect to HRP, SBP shows a higher stability and a lower susceptibility both to thermal and chemical inactivation, making it suitable for biotechnological applications (Ryan et al, 2006 ; Steevensz et al, 2014 ; Al-Maqdi et al, 2018 ; Bilal et al, 2018 ; Donadelli et al, 2018 ; Sadraei et al, 2019 ; Yang et al, 2019 ). In a previous study regarding the prospects for a large-scale soybean peroxidase commercialization, Hailu et al ( 2010 ) suggested that investments in an SBP extraction facility can be economically advantageous, estimating that, in a 0.5 ha plant, 6.2 metric tons of hulls can generate 0.56 billion units of crude SBP with a total annual revenue of 5.1 millions of CAD$.…”

Section: Introductionmentioning

confidence: 99%

A Way to Close the Loop: Physicochemical and Adsorbing Properties of Soybean Hulls Recovered After Soybean Peroxidase Extraction

et al. 2020

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Soybean hulls are one of the by-products of soybean crushing and find application mainly in the animal feed sector. Nevertheless, soybean hulls have been already exploited as source of peroxidase (soybean peroxidase, SBP), an enzyme adopted in a wide range of applications such as bioremediation and wastewater treatment, biocatalysis, diagnostic tests, therapeutics and biosensors. In this work, the soybean hulls after the SBP extraction, destined to become a putrescible waste, were recovered and employed as adsorbents for water remediation due to their cellulose-based composition. They were studied from a physicochemical point of view using different characterization techniques and applied for the adsorption of five inorganic ions [Fe(III), Al(III), Cr(III), Ni(II), and Mn(II)] in different aqueous matrixes. The behavior of the exhausted soybean hulls was compared to pristine hulls, demonstrating better performances as pollutant adsorbents despite significant changes in their features, especially in terms of surface morphology, charge and composition. Overall, this work evidences that these kinds of double-recovered scraps are an effective and sustainable alternative for metal contaminants removal from water.

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Section: Introductionmentioning

confidence: 99%

A Way to Close the Loop: Physicochemical and Adsorbing Properties of Soybean Hulls Recovered After Soybean Peroxidase Extraction

et al. 2020

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“…Soybean peroxidase (SBP) enzyme was immobilized on the APTES-functionalized photocatalytic supports (TiO 2 and ZnO particles), as depicted in Scheme 1 below, following a previously reported procedure [ 30 ]. Briefly, 3.0 g of commercially purchased TiO 2 and ZnO were mixed with ethanol/water (1:1) solution, and the solution was left under nitrogen and sonicated for a few minutes.…”

Section: Methodsmentioning

confidence: 99%

Immobilized Soybean Peroxidase Hybrid Biocatalysts for Efficient Degradation of Various Emerging Pollutants

et al. 2021

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In the present study, soybean peroxidase (SBP) was covalently immobilized onto two functionalized photocatalytic supports (TiO2 and ZnO) to create novel hybrid biocatalysts (TiO2-SBP and ZnO-SBP). Immobilization caused a slight shift in the pH optima of SBP activity (pH 5.0 to 4.0), whereas the free and TiO2-immobilized SBP showed similar thermal stability profiles. The newly developed hybrid biocatalysts were used for the degradation of 21 emerging pollutants in the presence and absence of 1-hydroxy benzotriazole (HOBT) as a redox mediator. Notably, all the tested pollutants were not equally degraded by the SBP treatment and some of the tested pollutants were either partially degraded or appeared to be recalcitrant to enzymatic degradation. The presence of HOBT enhanced the degradation of the pollutants, while it also inhibited the degradation of some contaminants. Interestingly, TiO2 and ZnO-immobilized SBP displayed better degradation efficiency of a few emerging pollutants than the free enzyme. Furthermore, a combined enzyme-chemical oxidation remediation strategy was employed to degrade two recalcitrant pollutants, which suggest a novel application of these novel hybrid peroxidase-photocatalysts. Lastly, the reusability profile indicated that the TiO2-SBP hybrid biocatalyst retained up to 95% degradation efficiency of a model pollutant (2-mercaptobenzothiazole) after four consecutive degradation cycles.

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“…Recently, the immobilization of HRP onto magnetic supports was considered as an innovative strategy for the development of biocatalysts for wastewaters treatment (Donadelli et al 2018). Magnetite nanoparticles' surface silanizing is the widely used approach for introducing surface functional groups (such as NH2) because of its properties, such as less-cytotoxicity, good stability, and ease of performance in organic and aqueous solvents (Hosseinifar et al 2017;Ramalingam et al 2018).…”

Section: Introductionmentioning

confidence: 99%

The assisting-approach of horseradish peroxidase immobilized onto amine-functionalized superparamagnetic iron oxide nanoparticles as a biocatalyst

Keshta

Gemeay

Khamis

2021

Preprint

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To enhance the removal of dye, horseradish peroxidase (HRP) was immobilized onto amine-functionalized superparamagnetic iron oxide and used as a biocatalyst for the oxidative degradation of Acid black-HC dye. The anchored enzyme was characterized by sets of techniques such as vibrating sample magnetometer, Fourier transform infrared, X-ray diffraction, thermogravimetric, scanning electron microscopy, BET and BJH methods, nitrogen adsorption-desorption measurements , Zeta potential, energy dispersive X-ray (EDX), and transmission electron microscopy. The Michaelis constant (K m ) values of free peroxidase and immobilized horseradish peroxidase were determined that equal 4.5 and 5 mM for hydrogen peroxide; 12.5 and 10 mM for guaiacol, respectively. Besides, the free peroxidase is thermally stable at 40°C however, the immobilized enzyme was up to 60°C. In the catalytic experiment, the immobilized HRP showed superior catalytic activity compared with free HRP for the oxidative decolorization and removal of Acid black-HC dye. The impacts of experimental parameters for instance catalyst dosage, pH, H 2 O 2 concentration, and temperature, were investigated. The reaction followed second-order kinetics and the thermodynamic activation parameters were determined.

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Soybean peroxidase immobilized onto silica-coated superparamagnetic iron oxide nanoparticles: Effect of silica layer on the enzymatic activity

Cited by 38 publications

References 36 publications

A Way to Close the Loop: Physicochemical and Adsorbing Properties of Soybean Hulls Recovered After Soybean Peroxidase Extraction

A Way to Close the Loop: Physicochemical and Adsorbing Properties of Soybean Hulls Recovered After Soybean Peroxidase Extraction

Immobilized Soybean Peroxidase Hybrid Biocatalysts for Efficient Degradation of Various Emerging Pollutants

The assisting-approach of horseradish peroxidase immobilized onto amine-functionalized superparamagnetic iron oxide nanoparticles as a biocatalyst

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