Soybean Oil: Genetic Approaches for Modification of Functionality and Total Content

Clemente, Tom; Cahoon, Edgar B.

doi:10.1104/pp.109.146282

Cited by 483 publications

(358 citation statements)

References 102 publications

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“…3 and Supplementary Table 13). The fatty acid biosynthesis pathway is composed of complex steps, and the modification of fatty acid biosynthesis genes regulates oil content or the ratio of oil components 27,28 . We identified 21 fatty acid biosynthesis genes in the selected regions (Supplementary Table 14).…”

Section: Supplementary Tables 8 and 9)mentioning

confidence: 99%

Resequencing 302 wild and cultivated accessions identifies genes related to domestication and improvement in soybean

et al. 2015

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Section: Supplementary Tables 8 and 9)mentioning

confidence: 99%

Resequencing 302 wild and cultivated accessions identifies genes related to domestication and improvement in soybean

et al. 2015

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“…The main reason that these mid-oleic genotypes have lower yield is most likely due to the weaker environmental adaptation ability of these FAD2-1 mutant plants [7]. The lesion in FAD2-1 alters the fatty acid profile of membranes in vegetative tissues.…”

Section: Discussionmentioning

confidence: 99%

Development of marker-free transgenic Jatrophaplants with increased levels of seed oleic acid

Mao

Chen

et al. 2012

Biotechnol Biofuels

117

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BackgroundJatropha curcas is recognized as a new energy crop due to the presence of the high amount of oil in its seeds that can be converted into biodiesel. The quality and performance of the biodiesel depends on the chemical composition of the fatty acids present in the oil. The fatty acids profile of the oil has a direct impact on ignition quality, heat of combustion and oxidative stability. An ideal biodiesel composition should have more monounsaturated fatty acids and less polyunsaturated acids. Jatropha seed oil contains 30% to 50% polyunsaturated fatty acids (mainly linoleic acid) which negatively impacts the oxidative stability and causes high rate of nitrogen oxides emission.ResultsThe enzyme 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine delta 12-desaturase (FAD2) is the key enzyme responsible for the production of linoleic acid in plants. We identified three putative delta 12 fatty acid desaturase genes in Jatropha (JcFAD2s) through genome-wide analysis and downregulated the expression of one of these genes, JcFAD2-1, in a seed-specific manner by RNA interference technology. The resulting JcFAD2-1 RNA interference transgenic plants showed a dramatic increase of oleic acid (> 78%) and a corresponding reduction in polyunsaturated fatty acids (< 3%) in its seed oil. The control Jatropha had around 37% oleic acid and 41% polyunsaturated fatty acids. This indicates that FAD2-1 is the major enzyme responsible for converting oleic acid to linoleic acid in Jatropha. Due to the changes in the fatty acids profile, the oil of the JcFAD2-1 RNA interference seed was estimated to yield a cetane number as high as 60.2, which is similar to the required cetane number for conventional premium diesel fuels (60) in Europe. The presence of high seed oleic acid did not have a negative impact on other Jatropha agronomic traits based on our preliminary data of the original plants under greenhouse conditions. Further, we developed a marker-free system to generate the transgenic Jatropha that will help reduce public concerns for environmental issues surrounding genetically modified plants.ConclusionIn this study we produced seed-specific JcFAD2-1 RNA interference transgenic Jatropha without a selectable marker. We successfully increased the proportion of oleic acid versus linoleic in Jatropha through genetic engineering, enhancing the quality of its oil.

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“…Soybean mutagenesis has previously been used for the successful improvement of seed oil composition [4][5][6]. In addition, novel alleles have been identified in germplasm collections of natural accessions, and the combination of these two sources of genetic variation has resulted in the identification of a number of soybean genotypes with modified oil content [2,3]. In some cases genetic lesions underlying these traits have been identified at the molecular level.…”

Section: Introductionmentioning

confidence: 99%

“…For these divergent applications, a modified fatty acid composition profile is sometimes desirable. For example, oil low in the saturated fatty acid palmitate has health benefits in food oils, and reduction of linolenic acid results in increased oxidative stability without the need for hydrogenation [2]. However, for some industrial applications, increased levels of saturated fatty acids (stearate and palmitate) could be desirable [3].…”

Section: Introductionmentioning

confidence: 99%

Soybean Oil-Quality Variants Identified by Large-Scale Mutagenesis

Hudson

2012

International Journal of Agronomy

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To identify genetic variation for fatty acid composition in mature soybean seeds, 4566 M 3 generation seed samples from a chemically mutagenized population were subjected to fatty acid profiling by gas chromatography. In the population, a wide range of variation in the content for each of the five major fatty acids was observed. Seventy-nine lines were identified which contained significantly high or low levels of one of the five major soybean fatty acids. These lines were advanced to the subsequent generation. Of the 79 lines showing a variant fatty acid profile in the M 3 , 52 showed clear heritability for the oil composition in the seeds of the subsequent generation. These lines are likely to represent 52 distinct genetic mutations. These mutants may represent new loci involved in the determination of soybean seed oil content or could be new isolates or alleles of previously identified genetic variants for soybean oil composition.

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Soybean Oil: Genetic Approaches for Modification of Functionality and Total Content

Cited by 483 publications

References 102 publications

Resequencing 302 wild and cultivated accessions identifies genes related to domestication and improvement in soybean

Resequencing 302 wild and cultivated accessions identifies genes related to domestication and improvement in soybean

Development of marker-free transgenic Jatrophaplants with increased levels of seed oleic acid

Soybean Oil-Quality Variants Identified by Large-Scale Mutagenesis

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