Soybean lipoxygenase inhibition by nonsteroidal antiinflammatory drugs

Sircar, J. C.; Schwender, Charles F.; Johnson, Elizabeth A.

doi:10.1016/0090-6980(83)90042-4

Cited by 81 publications

(47 citation statements)

References 6 publications

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“…Naproxen is a nonsteroidal anti-inflammatory drug that was used previously to inhibit LOX activity in cultured soybean cells (Creelman et al, 1992). In a study comparing 18 nonsteroidal anti-inflammatory drugs, naproxen was the best inhibitor of purified soybean LOX, having a greater affinity for the enzyme than did the substrate (Sircar et al, 1983). In the present work, tubers were initiated after 3 weeks by both control and naproxen-treated axillary buds, but the tubers that formed did not develop at the same rate.…”

Section: Effect Of a Lox Inhibitor On Tuber Growth In Vitromentioning

confidence: 68%

“…Twenty-three tubers produced from 15 control axillary bud cuttings and five tubers from 10 inhibitor-treated cuttings were pooled separately and evaluated. LOX activity was inhibited by incorporating 100 mol of naproxen, an inhibitor of LOX activity (Sircar et al, 1983), into the agar medium.…”

Section: Lox Inhibition Of In Vitro Tuberizationmentioning

confidence: 99%

“…Naproxen, a nonsteroidal antiinflammatory drug, is an effective inhibitor of plant LOX activity (Sircar et al, 1983;Creelman et al, 1992). Tuber formation was assayed in axillary buds of cuttings from nontransgenic plantlets of potato cv FL1607 grown in vitro.…”

Section: Lox Inhibition Of In Vitro Tuberizationmentioning

confidence: 99%

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Lipoxygenase Is Involved in the Control of Potato Tuber Development

et al. 2001

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Plant lipoxygenases (LOXs) are a functionally diverse class of dioxygenases implicated in physiological processes such as growth, senescence, and stress-related responses. LOXs incorporate oxygen into their fatty acid substrates and produce hydroperoxide fatty acids that are precursors of jasmonic acid and related compounds. Here, we report the involvement of the tuber-associated LOXs, designated the Lox1 class, in the control of tuber growth. RNA hybridization analysis showed that the accumulation of Lox1 class transcripts was restricted to developing tubers, stolons, and roots and that mRNA accumulation correlated positively with tuber initiation and growth. In situ hybridization showed that Lox1 class transcripts accumulated in the apical and subapical regions of the newly formed tuber, specifically in the vascular tissue of the perimedullary region, the site of the most active cell growth during tuber enlargement. Suppression mutants produced by expressing antisense coding sequence of a specific tuber LOX, designated POTLX-1 , exhibited a significant reduction in LOX activity in stolons and tubers. The suppression of LOX activity correlated with reduced tuber yield, decreased average tuber size, and a disruption of tuber formation. Our results indicate that the pathway initiated by the expression of the Lox1 class genes of potato is involved in the regulation of tuber enlargement. INTRODUCTIONTuber formation in potatoes is a complex developmental process that requires the interaction of environmental, biochemical, and genetic factors. Several important biological processes, such as carbon partitioning, signal transduction, and meristem determination are involved (Ewing and Struik, 1992). Under conditions of a short-day photoperiod and cool temperature, a transmissible signal is activated that initiates cell division and expansion and a change in the orientation of cell growth in the subapical region of the stolon tip (Ewing and Struik, 1992; Xu et al., 1998a). In this signal transduction pathway, perception of the appropriate environmental cues occurs in leaves and is mediated by phytochrome and gibberellins (van den Berg et al., 1995; Jackson and Prat, 1996; Jackson et al., 1996). van den Berg et al. (1996) detected at least 10 quanitative trait loci that control the ability to tuberize under long days, but none of these genes has been identified definitively.Tuber development at the stolon tip consists of biochemical and morphological processes. Both processes are controlled by differential gene expression (Hannapel, 1991; Bachem et al., 1996; Macleod et al., 1999), and most of the research in this area has focused on the biochemical processes, including starch synthesis (Abel et al., 1996;Preiss, 1996; Geigenberger et al., 1998) and storage protein accumulation (Mignery et al., 1984; Hendriks et al., 1991;Suh et al., 1991). Much less is known about the morphological controls of tuberization, although it is clear that phytohormones play a prominent role (Koda et al., 1991; Xu et al., 1998b;Sergeeva et ...

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Section: Effect Of a Lox Inhibitor On Tuber Growth In Vitromentioning

confidence: 68%

Section: Lox Inhibition Of In Vitro Tuberizationmentioning

confidence: 99%

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Lipoxygenase Is Involved in the Control of Potato Tuber Development

et al. 2001

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“…LOX has Переведено редколлегией been implicated in the progression of certain cancers and health disorders (Kelvakar et al 2000(Kelvakar et al , 2001. Furthermore, the products of LOX catalyzed oxygenation [hydro pero xy eico sa tetra enoic acid (HPETE), hydroxyl eicosa tetra eno ic acid (HETE), Leukotrines and Lipoxins) apparently are involved in the development of rheumatoid, arthritis, pso ria sis, asthmatic responses (Sircar et al 1983). LOX are sen si tive to antioxidants as antioxidants are involved in inhi bi tion of lipid hydroperoxide formation due to scavenging of lipidoxy or lipidperoxyradicals.…”

Section: Anti-lipoxygenase Radical Scavenging and Antimicrobial Actimentioning

confidence: 99%

Anti-lipoxygenase, Radical Scavenging and Antimicrobial Activities of Lichen Species of Genus Heterodermia (Physciaceae)

Behera¹,

Morey²,

Gaikwad³

2015

Bot Pac

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The aim of the research is to explore antilipoxygenase, radical scavenging and an ti mic ro bial activity of the ethyl acetate extract of the selected lichen spe cies of genus Heterodermia (Physciaceae . isidiophora showed the strong anti mic ro bial activity against most of the tested microorganisms at concentration ranging from 0.232 mg/ml to 0.591 mg/ml. These findings suggest that these li chen species of genus Heterodermia can be used as new bioresources for the na tu ral lipoxygenase inhibitor with antimicrobial and radical scavenging features. . Цель исследования -антилипоксигеназа, радикальная очистка и антимикробная активность этилацетатного экстракта видов лишайников ро да Heterodermia (Physciaceae). Экстрактам видов рода свойсвенно липоксигеназное инги бирование (LOX), зависящее от концентрации. Пятидесятипроцентное (IC 50 ) ингибирование LOX отмечалось у видов H. diademata, H. flabellata, H. an til la rum и H. incana при концентрациях 0,123 0,153, 0,160 и 0,150 мг/мл со от вет ст вен но; что меньше, чем у синтетических антиоксидантов BHA и BHT. Только экс тракт H. diademata показал IC 50 при концентрации 0,123 мг/мл равным LOX ин ги би тора индометацин. Кинетика LOX ингибирования экстрактов разных ви дов лишайников показывает разный режим ингибирования. Конкурентное ин ги бирование LOX отмечено у H. al bi cans и H. antillarum; неконкурентое -у H. dia de mata; отсутствие конкуренции характерно для H. angustiloba, H. fla bel la ta, H. in ca na, H. isidiophora и H. pseudospeciosa. Экстракты этих видов так же по ка за ли пятидесятипроцентную активность радикальной очистки. Этил аце тат ные экстракты H. diademata, H. angustiloba, H. albicans и H. isidiophora пока за ли силь ную антимикробную активность в отношении большинства про тес ти ро ван ных микроорганизмов в концентрациях от 0,232 до 0,591 мг/мл. Результаты по казывают, что виды лишайников Heterodermia могут быть использованы в ка честве нового биоресурса природной липоксигеназы с антимикробными и очистительными свойствами.

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“…5-LOX inhibitory assay was performed by using UV kinetic method [8,9]. This method was performed by using an assay mixture consisting 3 ml of 50 mM* phosphate buffer pH 6.3, along with 10 µl of 80 mM* of linoleic acid and potato 5-LOX enzymes.…”

Section: Lipoxygenase Assaymentioning

confidence: 99%

Anti-Inflammatory Activity of Manilkara Zapota Leaf Extract

Konuku

Karri

Gopalakrishnan

et al. 2017

Int J Curr Pharm Sci

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Objective: Manilkara zapota is a medicinal plant which is native to Mexico and Central America, and widely distributed in India. Various parts of this plant are traditionally used for treatment of several diseases, including inflammation-associated ailments. The main aim of the present study is to evaluate the anti-inflammatory potential of ethyl acetate and methanolic extracts of M. zapota leaf. Methods:In vitro secretary phospholipase A2 (PLA2) and 5-Lipoxygenase (5-LOX) assays and In vivo studies using carrageenan induced rat paw edema model were performed to assess the anti-inflammatory activity of M. zapota leaf extracts. Results:In vitro studies suggest that M. zapota leaf extracts exhibited significant SPLA2 and 5-LOX inhibitory activities. In in vivo studies M. zapota leaf extracts showed dose dependent inhibition of carrageenan induced paw edema in rats. The anti-inflammatory activity of ethyl acetate leaf extract was superior to methanolic extract. Conclusion:This study concluded that ethyl acetate leaf extract of M. zapota exhibited significant anti-inflammatory activity and warranted further investigation to isolate and identify the components.

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Soybean lipoxygenase inhibition by nonsteroidal antiinflammatory drugs

Cited by 81 publications

References 6 publications

Lipoxygenase Is Involved in the Control of Potato Tuber Development

Lipoxygenase Is Involved in the Control of Potato Tuber Development

Anti-lipoxygenase, Radical Scavenging and Antimicrobial Activities of Lichen Species of Genus Heterodermia (Physciaceae)

Anti-Inflammatory Activity of Manilkara Zapota Leaf Extract

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