Soybean Isoflavones Dose-Dependently Reduce Bone Turnover but Do Not Reverse Established Osteopenia in Adult Ovariectomized Rats

Picherit, Christel; Bennetau-Pelissero, Catherine; Chanteranne, Brigitte; Lebecque, Patrice; Davicco, Marie-Jeanne; Barlet, J.P.; Coxam, Véronique

doi:10.1093/jn/131.3.723

Cited by 101 publications

(59 citation statements)

References 21 publications

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“…Our results confirmed the findings of others that the OVX rat model was characterized by high bone turnover rate [20][21][22] and that FLL extract could effectively prevent the OVXinduced increase in bone turnover. The increase in bone turnover was the result of increase in both bone formation and bone resorption associated with E 2 deficiency.…”

Section: Discussionsupporting

confidence: 92%

Effects of Fructus Ligustri Lucidi Extract on Bone Turnover and Calcium Balance in Ovariectomized Rats

Zhang

Lai

Leung

et al. 2006

Biological & Pharmaceutical Bulletin

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Postmenopausal osteoporosis is a major age-related health problem for women who often have negative calcium balance due to decrease in intestinal calcium absorption, insufficient dietary calcium intake, as well as increase in urinary Ca loss associated with estrogen deficiency during menopause. 1)Hormone replacement therapy (HRT) has been an established regime for prevention of postmenopausal bone loss, 2,3) but recent evidence indicates that its long-term use is accompanied by side effects, such as the increased risk of breast, ovarian and endometrial cancer. 4,5) Thus, alternative means of proven efficacy and safety should be developed for prevention and treatment of postmenopausal osteoporosis.Traditional Chinese Medicine (TCM) has been widely used for thousands of years to treat fractures and joint diseases. Many herbs shown to have kidney-tonifying activities are used in TCM formulas for prevention and treatment of osteoporosis.6) Although these herbal medicines are seen as cost-effective alternatives by their traditional users, their international acceptance as a major regimen for prevention and treatment of osteoporosis would require extensive research using modern science.The source of the crude drug, Fructus Ligustri Lucidi (FLL, Chinese name, Nvzhenzi), is the fruit of Ligustrum lucidum Ait.7) It has been used in traditional Chinese medicine for over 1000 years, mainly to treat such ailments as menopausal problems, blurred vision, tinnitus, rheumatic pains, palpitations, backache, insomnia as well as to alleviate age-related symptoms.8-10) It was first described in the Chinese Materia Medica, Shen Nong Ben Cao Jing (Anonymous, ca. 200 B.C). In the theory of traditional Chinese medicine, it has the effects of maintaining healthy energy, and nourishing the liver and kidneys, is therefore a commonly prescribed herbal material in a number of formulae used to tonify the kidneys and strengthen bone. 11,12) Modern research has shown that FLL is useful for prevention of bone marrow loss in cancer patients receiving chemotherapy.13) Hao et al. observed that FLL induced ultrastructural changes on the corticotrophs of rat pituitary gland and provided morphological evidence for the action of FLL in modulating endocrine function.14) However, the beneficial effect on bone and mineral metabolism has not been scientifically evaluated.In this study, we investigated the effects of the crude FLL extract on bone and mineral metabolism, bone turnover marker and calcium balance in osteoporotic rats. The results provide evidence of the osteoprotective effects of this crude extract in ovariectomized rats. MATERIALS AND METHODS Measurement of Oleanolic Acid in Fructus LigustriLucidi (FLL) Extract Dried extract powder of Fructus Ligustri Lucidi was purchased from 999 Company (Shenzhen, China), and the yield of the extract from original plant was 42%. A voucher specimen was deposited in the Shenzhen Research Center of Traditional Chinese Medicine and Natural Products (Shenzhen, P.R.C.), where oleanolic acid was supplied. The con...

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Section: Discussionsupporting

confidence: 92%

Effects of Fructus Ligustri Lucidi Extract on Bone Turnover and Calcium Balance in Ovariectomized Rats

Zhang

Lai

Leung

et al. 2006

Biological & Pharmaceutical Bulletin

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“…Whereas estrogen prevent bone loss by reducing bone resorption, 28,29) the isoflavones genistein and ipriflavone, are antiresorptive only at chronic high doses and therefore have been reported to either inhibit or not affect the bone turnover rate. 30,31) Interestingly, neither of these isoflavones blocks the elevated bone resorption rates induced by ovariectomy or menopause. 32,33) Thus while isoflavone treatment may prevent bone loss, in part, because the enhanced bone formation exceeds resorption, the mechanism underlying the enhanced bone formation by isoflavone treatment in vivo is largely unknown.…”

Section: Discussionmentioning

confidence: 99%

Biochanin A Stimulates Osteoblastic Differentiation and Inhibits Hydrogen Peroxide-Induced Production of Inflammatory Mediators in MC3T3-E1 Cells

Lee

Choi

2005

Biological & Pharmaceutical Bulletin

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Phytoestrogens are plant chemicals that are structurally analogous to estrogen and are known to affect estrogenic activity. Biochanin A, a naturally occurring isoflavone, has been identified and detected in various diets and plant species. We examined the effects of biochanin A on the differentiation of osteoblastic MC3T3-E1 cells and the production of local factors in osteoblasts. Biochanin A (1-50 m mM) caused a significant elevation of cell growth, alkaline phosphatase (ALP) activity, collagen content, and osteocalcin secretion in osteoblastic MC3T3-E1 cells (pϽ0.05). The effect of biochanin A (10 m mM) in increasing ALP activity and collagen content was completely prevented by the presence of 10 Ϫ6 M cycloheximide and 10 Ϫ6 M tamoxifen, suggesting that biochanin A's effect results from a newly synthesized protein component and might be partly involved in estrogen action. We then examined the effect of biochanin A on the H 2 O 2 -induced production of inflammatory mediators in osteoblasts. Biochanin A (1-10 m mM) decreased the 0.2 mM H 2 O 2 -induced production of TNF-a a , IL-6 and NO in osteoblasts. These results suggest that biochanin A may be useful as potential phytoestrogens, which play important physiological roles in the prevention of postmenopausal osteoporosis.

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“…That is, a drastic change in bone turnover markers is observed in advance of change in bone mineral contents, especially shortly after OVX. Picherit et al 36) reported that the urine DPD level increased by 1.5-fold at 5.7 weeks after OVX and returned to the baseline at 11 weeks. Serum osteocalcin level also increased by 1.6-fold at 5.7 weeks, and returned to the baseline at 17 weeks.…”

Section: Discussionmentioning

confidence: 99%

Effects of Dietary Intake of Isoflavone Aglycone-rich Fermented Soybeans on Bone Metabolism in Ovariectomized Rats

Okabe

Tanimoto

2008

JOURNAL OF HEALTH SCIENCE

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We compared the effect of dietary intake of isoflavone aglycone-rich fermented soybeans (FS) with that of glucoside-rich non-fermented soybeans (NFS) on bone metabolism, using ovariectomized (OVX) rats. Female Sprague-Dawley rats were given OVX or a sham operation at 8 weeks of age. After a 2-week recovery, shamoperated rats were given a NFS diet for 57 days, while OVX rats were given the NFS or FS diet (Sham-NFS, OVX-NFS, and OVX-FS group, n = 10). The two diets had the same level of total isoflavone (228 µmol/100 g). The percentage of isoflavone aglycone to total isoflavone was 100% in the FS diet, and approximately 5% in the NFS diet. Tibial bone calcium and phosphorus contents were significantly lower in the OVX-NFS group than in the Sham-NFS group. This reduction was significantly prevented in the OVX-FS group. Tibial trabecular bone density was also significantly reduced by OVX, but the reduction tended to be prevented in the OVX-FS group (p = 0.1). The level of urinary deoxypyridinoline (DPD), a bone resorption marker, was significantly increased by OVX; this increase was significantly prevented in the OVX-FS group. Moreover, urinary total isoflavone levels tended to be higher in the OVX-FS group than in the OVX-NFS group (p = 0.1). In conclusion, this study suggested that intake of FS prevented excessive bone resorption and bone loss following OVX in rats. It was also suggested that these beneficial effects on bone resulted from higher absorption of isoflavone aglycone.

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Soybean Isoflavones Dose-Dependently Reduce Bone Turnover but Do Not Reverse Established Osteopenia in Adult Ovariectomized Rats

Cited by 101 publications

References 21 publications

Effects of Fructus Ligustri Lucidi Extract on Bone Turnover and Calcium Balance in Ovariectomized Rats

Effects of Fructus Ligustri Lucidi Extract on Bone Turnover and Calcium Balance in Ovariectomized Rats

Biochanin A Stimulates Osteoblastic Differentiation and Inhibits Hydrogen Peroxide-Induced Production of Inflammatory Mediators in MC3T3-E1 Cells

Effects of Dietary Intake of Isoflavone Aglycone-rich Fermented Soybeans on Bone Metabolism in Ovariectomized Rats

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