Soybean
 SAT1
 (
 Symbiotic Ammonium Transporter 1
 ) encodes a bHLH transcription factor involved in nodule growth and NH
 4
 +
 transport

Chiasson, David; Loughlin, Patrick C.; Mazurkiewicz, Danielle; Mohammadi‐Dehcheshmeh, Manijeh; Fedorova, Elena; Okamoto, Mamoru; McLean, Elizabeth; Glass, Anthony D. M.; Smith, Sally E.; Bisseling, Ton; Tyerman, Stephen D.; Day, David A.; Kaiser, Brent N.

doi:10.1073/pnas.1312801111

Cited by 87 publications

(67 citation statements)

References 43 publications

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“…Linked to the activity of a unique class of ammonium channels and to signaling cascades influencing a nodule circadian clock [51].…”

Section: Gmbhlhm1 Glycine Maxmentioning

confidence: 99%

Genome-wide survey of the bHLH super gene family in Brassica napus

Zhou

et al. 2020

BMC Plant Biol

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Background: The basic helix-loop-helix (bHLH) gene family is one of the largest transcription factor families in plants and is functionally characterized in diverse species. However, less is known about its functions in the economically important allopolyploid oil crop, Brassica napus. Results: We identified 602 potential bHLHs in the B. napus genome (BnabHLHs) and categorized them into 35 subfamilies, including seven newly separated subfamilies, based on phylogeny, protein structure, and exon-intron organization analysis. The intron insertion patterns of this gene family were analyzed and a total of eight types were identified in the bHLH regions of BnabHLHs. Chromosome distribution and synteny analyses revealed that hybridization between Brassica rapa and Brassica oleracea was the main expansion mechanism for BnabHLHs. Expression analyses showed that BnabHLHs were widely in different plant tissues and formed seven main patterns, suggesting they may participate in various aspects of B. napus development. Furthermore, when roots were treated with five different hormones (IAA, auxin; GA 3 , gibberellin; 6-BA, cytokinin; ABA, abscisic acid and ACC, ethylene), the expression profiles of BnabHLHs changed significantly, with many showing increased expression. The induction of five candidate BnabHLHs was confirmed following the five hormone treatments via qRT-PCR. Up to 246 BnabHLHs from nine subfamilies were predicted to have potential roles relating to root development through the joint analysis of their expression profiles and homolog function. Conclusion: The 602 BnabHLHs identified from B. napus were classified into 35 subfamilies, and those members from the same subfamily generally had similar sequence motifs. Overall, we found that BnabHLHs may be widely involved in root development in B. napus. Moreover, this study provides important insights into the potential functions of the BnabHLHs super gene family and thus will be useful in future gene function research.

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“…Linked to the activity of a unique class of ammonium channels and to signaling cascades influencing a nodule circadian clock [51].…”

Section: Gmbhlhm1 Glycine Maxmentioning

confidence: 99%

Genome-wide survey of the bHLH super gene family in Brassica napus

Zhou

et al. 2020

BMC Plant Biol

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“…Biolistic transformation of onion epidermal cells was performed as described (Campos-Soriano et al, 2011;Chiasson et al, 2014). Briefly, Zm-NPF6.4 or Zm-NPF6.6 was subcloned into the pBS-35S-attR-YFP vector and coated onto 0.6-mm gold microcarriers along with a plasma membrane marker, ECFP:Rop11 (Subramanian et al, 2006;Molendijk et al, 2008).…”

Section: Subcellular Localizationmentioning

confidence: 99%

Maize NPF6 Proteins Are Homologs of Arabidopsis CHL1 That Are Selective for Both Nitrate and Chloride

et al. 2017

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Nitrate uptake by plant cells requires both high- and low-affinity transport activities. nitrate transporter 1/peptide transporter family (NPF) 6.3 is a dual-affinity plasma membrane transport protein that has both high- and low-affinity functions. At-NPF6.3 imports and senses nitrate and is regulated by phosphorylation at Thr-101 (T101). A detailed functional analysis of two maize () homologs of At-NPF6.3 (Zm-NPF6.6 and Zm-NPF6.4) showed that Zm-NPF6.6 was a pH-dependent nonbiphasic high-affinity nitrate-specific transport protein. By contrast, maize NPF6.4 was a low-affinity nitrate transporter with efflux activity. When supplied chloride, NPF6.4 switched to a high-affinity chloride selective transporter, while NPF6.6 had only a low-affinity chloride transport activity. Structural predictions identified a nitrate binding His (H362) in NPF6.6 but not in NPF6.4. Mutation of NPF6.4 Tyr-370 to His (Y370H) resulted in saturable high-affinity nitrate transport activity and nitrate selectivity. Loss of H362 in NPF6.6 (H362Y) eliminated both nitrate and chloride transport. Furthermore, alterations to Thr-104, a conserved phosphorylation site in NPF6.6, resulted in a similar high-affinity nitrate transport activity with increased, whereas equivalent changes in NPF6.4 (T106) disrupted high-affinity chloride transport activity. NPF6 proteins exhibit different substrate specificity in plants and regulate nitrate transport affinity/selectivity using a conserved His residue.

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“…7B), revealing a connection between the clock and early stages of nodulation. Interestingly, GI, PRR5, and PRR7 were also found as differentially regulated in mature nodules formed by wild-type plants or roots silenced in a bHLH transcription factor (Chiasson et al, 2014). The authors suggested the existence of a nodule circadian clock that links availability of photosynthetic C and the demand of the nitrogen fixation process throughout the light/darkness cycle.…”

Section: Differential Expression Of Circadian Clock Genesmentioning

confidence: 99%

Changes in the Common Bean Transcriptome in Response to Secreted and Surface Signal Molecules of Rhizobium etli

et al. 2015

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Establishment of nitrogen-fixing symbiosis requires the recognition of rhizobial molecules to initiate the development of nodules. Using transcriptional profiling of roots inoculated with mutant strains defective in the synthesis of Nod Factor (NF), exopolysaccharide (EPS), or lipopolysaccharide (LPS), we identified 2,606 genes from common bean (Phaseolus vulgaris) that are differentially regulated at early stages of its interaction with Rhizobium etli. Many transcription factors from different families are modulated by NF, EPS, and LPS in different combinations, suggesting that the plant response depends on the integration of multiple signals. Some receptors identified as differentially expressed constitute excellent candidates to participate in signal perception of molecules derived from the bacteria. Several components of the ethylene signal response, a hormone that plays a negative role during early stages of the process, were down-regulated by NF and LPS. In addition, genes encoding proteins involved in small RNA-mediated gene regulation were regulated by these signal molecules, such as Argonaute7, a specific component of the trans-acting short interfering RNA3 pathway, an RNA-dependent RNA polymerase, and an XH/XP domaincontaining protein, which is part of the RNA-directed DNA methylation. Interestingly, a number of genes encoding components of the circadian central oscillator were down-regulated by NF and LPS, suggesting that a root circadian clock is adjusted at early stages of symbiosis. Our results reveal a complex interaction of the responses triggered by NF, LPS, and EPS that integrates information of the signals present in the surface or secreted by rhizobia.

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Soybean SAT1 ( Symbiotic Ammonium Transporter 1 ) encodes a bHLH transcription factor involved in nodule growth and NH ₄ ⁺ transport

Cited by 87 publications

References 43 publications

Genome-wide survey of the bHLH super gene family in Brassica napus

Genome-wide survey of the bHLH super gene family in Brassica napus

Maize NPF6 Proteins Are Homologs of Arabidopsis CHL1 That Are Selective for Both Nitrate and Chloride

Changes in the Common Bean Transcriptome in Response to Secreted and Surface Signal Molecules of Rhizobium etli

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Soybean SAT1 ( Symbiotic Ammonium Transporter 1 ) encodes a bHLH transcription factor involved in nodule growth and NH 4 + transport

Cited by 87 publications

References 43 publications

Genome-wide survey of the bHLH super gene family in Brassica napus

Genome-wide survey of the bHLH super gene family in Brassica napus

Maize NPF6 Proteins Are Homologs of Arabidopsis CHL1 That Are Selective for Both Nitrate and Chloride

Changes in the Common Bean Transcriptome in Response to Secreted and Surface Signal Molecules of Rhizobium etli

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Soybean SAT1 ( Symbiotic Ammonium Transporter 1 ) encodes a bHLH transcription factor involved in nodule growth and NH ₄ ⁺ transport