Soybean (Glycine max) WRINKLED1 transcription factor, GmWRI1a, positively regulates seed oil accumulation

Chen, Liang; Zheng, Yuhong; Dong, Zhimin; Meng, Fanzhao; Sun, Xin; Fan, Xuhong; Zhang, Yunfeng; Wang, Mingliang; Wang, Shuming

doi:10.1007/s00438-017-1393-2

Cited by 43 publications

(39 citation statements)

References 53 publications

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“…A total of 181 genes were located within the LD regions of the top-significance SNPs or the co-localized SNPs in two populations. In previous studies (Gutiérrez-Marcos et al 2007, Huang et al 2012, Sosso et al 2015, Doll et al 2017, Chen et al 2018, the orthologs of the five genes (Zm00001d007774, Zm00001d027854, Zm00001 d047868, Zm00001d021993 and Zm00001d012585) have been demonstrated to regulate KMC or seed development in plant species (Table 4). In order to verify the reliability of the five candidate genes, we separately calculated the linkage coefficients (D') between the significant SNPs and each of the SNPs located in the above five genes.…”

Section: Intragenic Variations Affecting Kmcmentioning

confidence: 95%

Combined linkage mapping and association analysis reveals genetic control of maize kernel moisture content

Zhang

Guan

et al. 2020

Physiologia Plantarum

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The free moisture in crop kernels after being naturally dried is referred to as kernel moisture content (KMC). Maize KMC reflects grain quality and influences transportation and storage of seeds. We used an IBM Syn10 DH maize population consisting of 249 lines and an association panel comprising 310 maize inbred lines to identify the genetic loci affecting maize KMC in three environments. Using the IBM population detected 13 QTL on seven chromosomes, which were clustered into nine common QTL. Genome-wide association analysis (GWAS) identified 16 significant SNPs across the 3 environments, which were linked to 158 genes across the three environments. Combined QTL mapping and GWAS found two SNPs that were located in two of the mapped QTL, respectively. Twenty-three genes were linked with the loci co-localized in both populations. Of these 181 genes, five have previously been reported to be associated with KMC or to regulate seed development. These associations were verified by candidate gene association analysis. Two superior alleles and one favorable haplotype for Zm00001d007774 and Zm00001d047868 were found to influence KMC. These findings provide insights into molecular mechanisms underlying maize KMC and contribute to the use of markerassisted selection for breeding low-KMC maize.

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Section: Intragenic Variations Affecting Kmcmentioning

confidence: 95%

Combined linkage mapping and association analysis reveals genetic control of maize kernel moisture content

Zhang

Guan

et al. 2020

Physiologia Plantarum

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“…WRI1 recognizes the AW box [C n T n G (n) 7CG] nucleotide sequence present in proximal upstream regions of target genes (Maeo et al, 2009). WRI1 has an essential role in fatty acid biosynthesis, and its overexpression enhances seed oil content in Arabidopsis thaliana (Baud et al, 2009;Cernac & Benning, 2004), rapeseed (Brassica napus) (Liu et al, 2010), maize (Zea mays) (Pouvreau et al, 2011;Shen et al, 2010), Camelina sativa , oil palm (Elaeis guineensis) (Yeap et al, 2017), and soybean (Chen et al, 2018). WRI1 regulates the expression of PK and BCCP2, which are downregulated in wri1 mutant arabidopsis seeds (Baud et al, 2007;Ruuska et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…WRI1 regulates the expression of PK and BCCP2, which are downregulated in wri1 mutant arabidopsis seeds (Baud et al, 2007;Ruuska et al, 2002). In soybean, GmWRI1 binds to the proximal upstream region of GmKAS1, which is upregulated in GmWRI1overexpressor seeds (Chen et al, 2018). The dehydrationresponsive element (DRE) induces plant defense genes to promote adaptation and survival in response to drought, salt, cold, and heat stresses (Sakuma et al, 2006;Yamaguchi-Shinozaki & Shinozaki, 1994).…”

Section: Introductionmentioning

confidence: 99%

High temperature during soybean seed development differentially alters lipid and protein metabolism

Nakagawa

Ario

Tomita

et al. 2020

Plant Production Science

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High temperatures during seed development can affect the seed yield and quality in many crops. Here, we analyzed how high temperature alters the main seed storage compounds (lipid and protein) in soybean. At five days after R5 stage (initial seed filling stage), soybean plants were treated with control (20/20ºC day/night) and high temperature (30/30ºC day/night). After treatment, immature seed was sampled, analyzed for lipid and protein contents and for expression of seed storage compounds related genes. High temperature during seed filling increased lipid content but decreased protein content, associating with yield reduction. It increased the expression of two genes related to seed lipid biosynthesis (GmBCCP2 and GmKAS1) and genes for a lipid biosynthesis regulator (GmWRI1) and its transcription factor (GmDREBL), and decreased the expression of genes related to lipid degradation such as GmACXs. High temperature downregulated genes related to seed storage protein (GmGy1, GmGy2, GmGy4, GmGy5 and Gmβ-conglycinin) and upregulated genes for cysteine and aspartate proteinases. Therefore, high temperature during seed filling preferentially accumulates lipid than protein content in seed, although seed yield reduction was associated with lower seed protein content in soybean. Our study provides insights for further improvements of soybean seed oil under abiotic stress such as heat stress.

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“…Further studies showed that AtWRI1 also plays a role in processes such as lipid assembly, storage, flowering time, seed development, embryo maturation, plant hormone regulation, and photosynthesis (Cernac et al, 2006;Kong et al, 2017;Li et al, 2015;Maeo et al, 2009;Wu et al, 2014). A number of other communications have also revealed orthologs of AtWRI1 being involved in plant oil biosynthesis (Adhikari et al, 2016;Bhattacharya et al, 2016;Chen et al, 2017;Ivarson et al, 2016;Kang et al, 2017;Kim et al, 2016;Liu et al, 2010;Ma et al, 2013;Pouvreau et al, 2011;Shen et al, 2010;Vanhercke et al, 2013;Wu et al, 2014;Yeap et al, 2017). For example, expression of the Brassica napus WRI1(BnWRI1) in Arabidopsis promoted between 10% and 40% increased seed oil content and led to larger seed and size mass (Liu et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Transient coexpression of AtWRI1 and AtDGAT in tobacco leaves shifted polyunsaturated to monounsaturated fatty acids and increased TAG biosynthesis in leaves (Vanhercke et al, 2013). Under control of the seed specific napin promoter, the soybean GmWRI1 increased oleic and linoleic leading to increased oil content in soybean (Chen et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Expression of the Arabidopsis WRINKLED 1 transcription factor leads to higher accumulation of palmitate in soybean seed

Vogel

Noyer

Park

et al. 2019

Plant Biotechnology Journal

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Summary Soybean ( Glycine max [L.] Merr.) is a commodity crop highly valued for its protein and oil content. The high percentage of polyunsaturated fatty acids in soybean oil results in low oxidative stability, which is a key parameter for usage in baking, high temperature frying applications, and affects shelf life of packaged products containing soybean oil. Introduction of a seed‐specific expression cassette carrying the Arabidopsis transcription factor WRINKLED 1 (At WRI 1) into soybean, led to seed oil with levels of palmitate up to approximately 20%. Stacking of the At WRI 1 transgenic allele with a transgenic locus harbouring the mangosteen steroyl‐ ACP thioesterase (GmFatA) resulted in oil with total saturates up to 30%. The creation of a triple stack in soybean, wherein the At WRI 1 and GmFatA alleles were combined with a FAD 2‐1 silencing allele led to the synthesis of an oil with 28% saturates and approximately 60% oleate. Constructs were then assembled that carry a dual FAD 2‐1 silencing element/GmFatA expression cassette, alone or combined with an At WRI 1 cassette. These plasmids are designated pPTN 1289 and pPTN 1301, respectively. Transgenic events carrying the T‐ DNA of pPTN 1289 displayed an oil with stearate levels between 18% and 25%, and oleate in the upper 60%, with reduced palmitate (<5%). While soybean events harboring transgenic alleles of pPTN 1301 had similar levels of stearic and oleate levels as that of the pPTRN 1289 events, but with levels of palmitate closer to wild type. The modified fatty acid composition results in an oil with higher oxidative stability, and functionality attributes for end use in baking applications.

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Soybean (Glycine max) WRINKLED1 transcription factor, GmWRI1a, positively regulates seed oil accumulation

Cited by 43 publications

References 53 publications

Combined linkage mapping and association analysis reveals genetic control of maize kernel moisture content

Combined linkage mapping and association analysis reveals genetic control of maize kernel moisture content

High temperature during soybean seed development differentially alters lipid and protein metabolism

Expression of the Arabidopsis WRINKLED 1 transcription factor leads to higher accumulation of palmitate in soybean seed

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