Soybean Glyceollins and Human Health

Ohanenye, Ikenna C.; Okagu, Innocent Uzochukwu; Ogunrinola, Oluwaseyi A.; Agboinghale, Precious Eseose; Udenigwe, Chibuike C.

doi:10.1201/9781003030294-7

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The most well-known phytoalexins that are clinical drugs include the anticancer metabolite taxol [ 8 ], the antidiabetic berberine [ 9 ], and the antimalarial artemisinin [ 10 ]. Many other phytoalexins are in (pre)clinical testing [ 5 , 11 , 12 ], including the glyceollins from soybean that inhibit the survival and proliferation of several cancer cell and tumor types by mechanisms that are distinct from conventional therapeutics [ 13 , 14 , 15 ]. Additionally, glyceollins hold promise as natural antimicrobials in the swine industry [ 16 , 17 ], yet exhibit neuroprotective activities in mammalian cells [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

RNA-Seq Dissects Incomplete Activation of Phytoalexin Biosynthesis by the Soybean Transcription Factors GmMYB29A2 and GmNAC42-1

Lin

Monsalvo

et al. 2023

Plants

View full text Add to dashboard Cite

Glyceollins, isoflavonoid-derived antimicrobial metabolites, are the major phytoalexins in soybean (Glycine max). They play essential roles in providing resistance to the soil-borne pathogen Phytophthora sojae and have unconventional anticancer and neuroprotective activities that render them desirable for pharmaceutical development. Our previous studies revealed that the transcription factors GmMYB29A2 and GmNAC42-1 have essential roles in activating glyceollin biosynthesis, yet each cannot activate the transcription of all biosynthesis genes in the absence of a pathogen elicitor treatment. Here, we report that co-overexpressing both transcription factors is also insufficient to activate glyceollin biosynthesis. To understand this insufficiency, we compared the transcriptome profiles of hairy roots overexpressing each transcription factor with glyceollin-synthesizing roots treated with wall glucan elicitor (WGE) from P. sojae. GmMYB29A2 upregulated most of the WGE-regulated genes that encode enzymatic steps spanning from primary metabolism to the last step of glyceollin biosynthesis. By contrast, GmNAC42-1 upregulated glyceollin biosynthesis genes only when overexpressed in the presence of WGE treatment. This is consistent with our recent discovery that, in the absence of WGE, GmNAC42-1 is bound by GmJAZ1 proteins that inhibit its transactivation activity. WGE, and not GmMYB29A2 or GmNAC42-1, upregulated the heat shock family gene GmHSF6-1, the homolog of Arabidopsis HSFB2a that directly activated the transcription of several glyceollin biosynthesis genes. Our results provide important insights into what biosynthesis genes will need to be upregulated to activate the entire glyceollin biosynthetic pathway.

show abstract