Soybean Germplasm Resistant to Races 1 and 2 of Heterodera glycines

Abstract

Heterodera glycines Ichinohe is a serious pest of soybean [Glycine max (L.) Merr.] in the USA. Several Races of H. glycines occur in the soybean growing areas. Soybean accessions with resistance to Races 3, 5, and 14 have been identified. Data on reaction of these accessions for Races 1 and 2 are not available. Our objective was to bioassay these accessions for resistance to H. glycines Races 1 and 2. Eighty‐six accessions, host differentials, and susceptible control were bioassayed during 1995 to 1996 for each of the two Races in thermoregulated water baths in the greenhouse. The results indicated that 52 accessions had resistance to H. glycines Race 1, and 24 accessions had resistance to Race 2, respectively. Soybean PIs 89772, 90763, 404166, 404198A, 437654, 437690, 438489B, 567491A, and 567516C were either resistant or moderately resistant to both Races. These are potential resistance sources to H. glycines Races 1, and 2 for developing resistant soybean cultivars. These PI lines are being fingerprinted by means of molecular markers to identify the unique lines to allow broadening the diversity of resistance gene utilization.

Stacking Resistance Alleles from Wild and Domestic Soybean Sources Improves Soybean Cyst Nematode Resistance

“…Because of the importance of SCN, there has been focused research on host resistance to this pest. This research has led to the identification of at least 118 SCN resistant soybean plant introductions from the USDA-ARS soybean germplasm collection (Arelli et al, 2000). Some of these resistance sources have been used in breeding programs and resistant cultivars have been available in the United States since 1965 (Brim and Ross, 1966).…”

Investigation of Soybean Cyst Nematode Heterodera Glycines Type and Evaluation of Resistance on Soybean Varieties and Germplasms in Korea

“…1. Soybean cyst nematodes were increased from the five different soil samples to conduct further SCN test in the greenhouse (A), and Heterodera glycines type test were performed on SCN check varieties as described by Arelli et al (2000), Niblack et al (2002) and Kim et al (2011) in the thermo-regulated water bath system (B).…”

“…Soybean cyst nematode populations were first reproduced to get the high density SCN populations from originally collected soil samples, and were cultured by a susceptible host soybean variety Lee 74 in one liter plastic beaker maintained with 27℃ temperature in a thermo-regulated water bath system on May 2012 in the greenhouse of the Gyeongsang National University as described by Arelli et al (2000) and Niblack et al (2002). The PVC tubes (16.5 cm length and 2.95 cm inside diameter) were filled with autoclaved sandy soil (soil: sand ratio 2:1) and packed into plastic crocks that were suspended over a water bath maintained at constant 27°C temperature condition.…”

Nematode Resistance in Soybean

“…With over 19,000 plant introductions (PIs), the USDA Soybean Germplasm Collection (http://www.ars-grin.gov) has been a valuable resource in finding sources of SCN resistance. Efforts have been made to evaluate this collection for new sources of resistance to either single-or multi-race SCN populations (Anand and Gallo 1984;Anand et al 1988;Arelli et al 2000;Diers et al 1997;Epps and Hartwig 1972;Rao-Arelli et al 1997;Ross and Brim, 1957;Young 1990). In a field study conducted soon after SCN was reported in the United States, Ross and Brim (1957) reported PI 88788 and Peking (PI 548402) as new sources of SCN resistance.…”