Soybean Generations Under Gamma Rays and Effects on Seed Quality

Abstract

Mutation induction is used to improve existing or generate new varieties. The objective of this study was to evaluate the effect of gamma radiation doses under successive generations of cultivation on seed quality of two soybean lines (VX04-6828 and VX04-5692). Different gamma-ray doses (60Co) were evaluated (0; 50; 150 and 250 Gray) in the M0 (first sowing) generation, and analyzed for water content, germination, vigor, abnormal seedlings, dead seeds, and hilum color. Then, sowing (M0) was carried out to obtain plants and seeds of the first production cycle (M1 generation), and then those of the subsequent cycle (M2 generation). The same analyses of M0 were performed for M2 and M3 generations, with the addition of germination rate and emergence speed index in the field. The data were submitted to analysis of variance in a factorial arrangement. The generations were compared using Tukey’s test, while doses were evaluated by regression analysis. Seeds of the two soybean lines showed significant interaction for the different generations and gamma-ray doses in all the variables analyzed, except for water content. The application of gamma rays in soybean seeds showed effects on successive generations of cultivation, with better physiological quality up to the dose of 150 Gy (Gray) and greater sensitivity to gamma radiation for the VX04-5692 line.

Gamma irradiation of eggplant seeds influences plant growth, yield and nutritional profile in M1 generation

“…The preservation, decrease, or increase in agromorphological traits, mineral and nutrient compositions of plants grown from irradiated eggplant seeds compared to the control plants made it a problematic task to clearly highlight the behaviour of eggplants in response to gamma ray irradiation. Consistent with our study, studies in different plants show that variations in growth and yield traits in response to gamma ray irradiation is dependent on the crop variety as well as the radiation dose (Rozman, 2014;Majeed et al, 2018;Aparecida Costa Nobre et al, 2022;Puripunyavanich et al, 2022;Saibari et al, 2023) and as such it is difficult to establish a standard dose for mutation breeding in eggplants.…”

Morphological Characteristics of Ornamental Chilli Mutants Irradiated by Gamma Ray

“…(Parlaongan et al, 2022). In several studies, high doses have resulted in growth abnormalities, germination delays, and lethality in susceptible plants, so Lethal Dose 50 is a parameter for the occurrence of 50% mortality in genetic material irradiated with gamma rays, and it is hoped that a lot of diversity will form at this dose (Nobre et al, 2022). A higher LD 50 can result in a higher level of plant lethality.…”

“…Gamma irradiation is one of the most commonly used physical mutagens. Gamma-ray irradiation has been widely used in various plants for various purposes, increasing genetic diversity and tolerance of abiotic and biotic stresses and increasing the quantity and quality of yields, such as seedless citrus breeding (Goldenberg et al, 2014), tomato (Wiendl et al, 2013), soybean (Nobre et al, 2022), rice (Kato et al, 2020), buckwheat (Tabosa et al, 2007), dan wheat (Kiani et al, 2022).…”