Soybean CCA1-like MYB transcription factor GmMYB133 modulates isoflavonoid biosynthesis

Bian, Shaomin; Li, Ruihua; Xia, Siqi; Liu, Yajing; Jin, Dong; Xie, Xin; Dhaubhadel, Sangeeta; Zhai, Limin; Wang, Jingying; Li, Xuyan

doi:10.1016/j.bbrc.2018.11.033

Cited by 31 publications

(37 citation statements)

References 26 publications

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“…The transcription factors of the MYB family play crucial roles in the regulation of isoflavone biosynthesis. GmMYB29, GmMYB102, GmMYB133, GmMYB176, GmMYB280, and GmMYB502 are positive regulators of isoflavone biosynthetic genes (Anguraj Vadivel et al, 2019;Bian et al, 2018;Chu et al, 2017;Sarkar et al, 2019;Yi et al, 2010), while GmMYB29B, GmMYB39, and GmMYB100 negatively regulate those genes (Jahan et al, 2020;Liu et al, 2013;Yan et al, 2015). Among these MYBs, GmMYB176 showed the highest expression level in the roots (Figure 7, Table S8).…”

Section: Diurnal Variation Of Biosynthetic Gene Expression For Isofmentioning

confidence: 98%

“…For example, RVE8 positively regulates anthocyanin biosynthesis (Pérez-García et al, 2015), and PRR5/7/9 negatively regulates carotenoid and abscisic acid biosynthesis in Arabidopsis (Fukushima et al, 2009). In soybeans, CCA1-like MYB transcription factor GmMYB133 (Glyma.07G066100) stimulates isoflavone biosynthesis by inducing chalcone synthase 8 (CHS8) and isoflavone synthase 2 (IFS2) (Bian et al, 2018), suggesting the diurnal regulation of isoflavone biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

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Diurnal metabolic regulation of isoflavones and soyasaponins in soybean roots

et al. 2020

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Section: Diurnal Variation Of Biosynthetic Gene Expression For Isofmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Diurnal metabolic regulation of isoflavones and soyasaponins in soybean roots

et al. 2020

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“…Mutant collections are being screened in order to develop new commercial soybean varieties [ 144 ]. Some positive [ 143 , 145 , 146 , 147 , 148 , 149 ] or negative [ 150 , 151 ] regulators of isoflavonoid biosynthesis in soybean have already been found; however, there are still many aspects and actors of the regulation of this pathway that have to be discovered. We expect that the advancement produced with the model legume L. japonicus will help to make progress in the understanding of isoflavonoid biosynthesis regulation in soybean.…”

Section: The Importance Of Isoflavonoid Biosynthesis In Soybean: Umentioning

confidence: 99%

Flavonoids and Isoflavonoids Biosynthesis in the Model Legume Lotus japonicus; Connections to Nitrogen Metabolism and Photorespiration

García‐Calderón

Pérez-Delgado

Paľove-Balang

et al. 2020

Plants

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Phenylpropanoid metabolism represents an important metabolic pathway from which originates a wide number of secondary metabolites derived from phenylalanine or tyrosine, such as flavonoids and isoflavonoids, crucial molecules in plants implicated in a large number of biological processes. Therefore, various types of interconnection exist between different aspects of nitrogen metabolism and the biosynthesis of these compounds. For legumes, flavonoids and isoflavonoids are postulated to play pivotal roles in adaptation to their biological environments, both as defensive compounds (phytoalexins) and as chemical signals in symbiotic nitrogen fixation with rhizobia. In this paper, we summarize the recent progress made in the characterization of flavonoid and isoflavonoid biosynthetic pathways in the model legume Lotus japonicus (Regel) Larsen under different abiotic stress situations, such as drought, the impairment of photorespiration and UV-B irradiation. Emphasis is placed on results obtained using photorespiratory mutants deficient in glutamine synthetase. The results provide different types of evidence showing that an enhancement of isoflavonoid compared to standard flavonol metabolism frequently occurs in Lotus under abiotic stress conditions. The advance produced in the analysis of isoflavonoid regulatory proteins by the use of co-expression networks, particularly MYB transcription factors, is also described. The results obtained in Lotus japonicus plants can be also extrapolated to other cultivated legume species, such as soybean, of extraordinary agronomic importance with a high impact in feeding, oil production and human health.

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“…In soybean, several MYB transcription factors have been reported to regulate the key isoflavonoid biosynthetic genes and affect isoflavonoid accumulation. Overexpression of GmMYB29 (Chu et al, 2017), GmMYB133 (Bian et al, 2018), GmMYB12B2 (Li et al, 2013), GmMYB58 and GmMYB205 (Han et al, 2017) play a positive role in the regulation of isoflavonoid biosynthesis while GmMYB100 (Yan et al, 2015) and GmMYB39 (Liu et al, 2013) inhibit isoflavonoid accumulation by suppressing the expression of multiple isoflavonoid genes. Previously we showed that down regulation of GmCHS8 expression reduced isoflavonoid levels in soybean hairy roots (Yi et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

GmMYB176 Regulates Multiple Steps in Isoflavonoid Biosynthesis in Soybean

et al. 2019

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Isoflavonoids are a group of plant natural compounds synthesized almost exclusively by legumes, and are abundant in soybean seeds and roots. They play important roles in plant-microbial interactions and the induction of nod gene expression in Rhizobia that form nitrogen-fixing nodules on soybean roots. Isoflavonoids also contribute to the positive health effects associated with soybean consumption by humans and animals. An R1 MYB transcription factor GmMYB176 regulates isoflavonoid biosynthesis by activating chalcone synthase ( CHS ) 8 gene expression in soybean. Using a combination of transcriptomic and metabolomic analyses of GmMYB176-RNAi silenced (GmMYB176-Si), GmMYB176-overexpressed (GmMYB176-OE), and control soybean hairy roots, we identified a total of 33 differentially expressed genes (DEGs) and 995 differentially produced metabolite features (DPMF) in GmMYB176-Si hairy roots, and 5727 DEGs and 149 DPMFs in GmMYB176-OE hairy roots. By a targeted approach, 25 isoflavonoid biosynthetic genes and 6 metabolites were identified as differentially regulated in GmMYB176-OE and GmMYB176-Si soybean hairy roots. Taken together, our results demonstrate the complexity of isoflavonoid biosynthesis in soybean roots and suggest that a coordinated expression of pathway genes, substrate flux and product threshold level may contribute to the dynamic of the pathway regulation.

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Soybean CCA1-like MYB transcription factor GmMYB133 modulates isoflavonoid biosynthesis

Cited by 31 publications

References 26 publications

Diurnal metabolic regulation of isoflavones and soyasaponins in soybean roots

Diurnal metabolic regulation of isoflavones and soyasaponins in soybean roots

Flavonoids and Isoflavonoids Biosynthesis in the Model Legume Lotus japonicus; Connections to Nitrogen Metabolism and Photorespiration

GmMYB176 Regulates Multiple Steps in Isoflavonoid Biosynthesis in Soybean

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