Soybean Callus—A Potential Source of Tocopherols

Abstract

In vitro cultures have been used as an effective means to achieve a high level of secondary metabolites in various plants, including soy. In this study, the contents of α-, γ-, and δ- tocopherol were quantified in soybean callus, and their amounts were compared to those of soybeans cultivated using the conventional tillage system with three weed controls (respectively without herbicide and with two variants of herbicide). Soybean callus was produced using Murashige and Skoog 1962 (MS) medium supplemented with 0.1 mg/L 6-Benzylaminopurine (BAP) and 0. 1 mg/L Thidiazuron (TDZ). The highest amount of fresh callus was obtained from soybeans from the conventional tillage system with second weed control (S-metolachlor 960 g/L, imazamox 40 g/L, and propaquizafop 100 g/L) respectively 13,652.4 ± 1177.62 mg. The analyzed tocopherols were in much higher content in soy dry callus than the soybean seeds (5.63 µg/g compared with the 0.35 α-toco in soybean, 47.57 µg/g compared with 18.71 µg/g γ-toco or, 5.56 µg/g compared with 1.74 µg/g β-toco). The highest content of the three analyzed tocopherols was γ -tocopherol, both in callus and soybeans. Furthermore, the data showed that herbicides used in soybean culture significantly influenced both the in vitro callus production and the tocopherol callus content (p ˂ 0.05). Altogether, soybean callus can be an important source of tocopherols, and herbicides significantly influence in vitro callus production and the tocopherol callus content.

Antioxidant Green Factories: Toward Sustainable Production of Vitamin E in PlantIn VitroCultures

“…The potential of using in vitro callus generated from soybeans has also recently been showcased. 148 Though authors of the study obtained a higher percentage of tocopherols in the callus than the seed, the callus line was predominated by γ-tocopherol. An analysis of callus generated from tocopherol-rich leaf regions showed a better percentage concentration of α-tocopherol.…”

“…Callus/suspension cultures induced from safflower are also commonly studied for the same application. The potential of using in vitro callus generated from soybeans has also recently been showcased . Though authors of the study obtained a higher percentage of tocopherols in the callus than the seed, the callus line was predominated by γ-tocopherol.…”

Utilization of antioxidants and anti-inflammatory compounds in managing SARS-CoV-2 infection to achieve healthy community: A review

“…Studies suggested that naturally occurring αtocopherol has a notable effect on human health compared with synthetic ones. Due to the mentioned facts, scientists and researchers around the globe are studying plants that exhibit effective vitamin E content [17][18][19].…”

“…A study suggested that adults take 15 mg of naturally occurring alpha-tocopherol (RRR α-tocopherol) daily. The anti-inflammatory potential of tocopherol has been studied for several areas of human health, including the immune system, cancer, the central nervous system, and the cardiovascular system [15,18,19].…”