Soyasaponin-I-modified invasive behavior of cancer by changing cell surface sialic acids

Hsu, Chi-Cheng; Lin, Ting‐Yi; Chang, Wei-Wei; Wu, Ching‐Yuan; Lo, Wen-Ling; Wang, Peng-Hui; Tsai, Ying-Chieh

doi:10.1016/j.ygyno.2004.10.010

Cited by 87 publications

(58 citation statements)

References 32 publications

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“…The spontaneous metastasis of murine cells was found to be associated with sialic acid content of the cell culture and the degree exposed on the tumor cell surface [31]. A recent study suggested that inhibition of cell surface sialic acid might provide an anti-metastatic therapeutic target [32].…”

Section: Discussionmentioning

confidence: 99%

Tissue and serum α2-3- and α2-6-linkage specific sialylation changes in oral carcinogenesis

et al. 2007

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Increased sialylation of cell surface glycoconjugates is among the key molecular changes associated with malignant transformation and cancer progression. We investigated significance of linkage-specific sialylation changes in oral carcinogenesis. Tissue and serum levels of total sialic acid (TSA), linkage-specific sialyltransferases (ST) and sialoproteins were analyzed from patients with oral precancerous conditions (OPC) and oral cancer as well as the post-treatment follow-up blood samples of oral cancer patients. TSA levels were measured using a spectrophotometric method. The linkage-specific lectins, Sambusus nigra (SNA) and Maackia amurensis (MAM) detects alpha 2-6- and alpha 2-3-linked sialic acid, respectively, were used to analyze ST activity and sialoproteins. Malignant tissues showed significantly higher levels of TSA, reactivity of SNA and MAM, and alpha 2,3-ST activity compared to the adjacent normal tissues. alpha 2,6-ST was also higher in malignant tissues. Similarly, the marker levels were higher in precancerous tissues than their adjacent normal tissues. Serum levels of TSA, TSA/ total proteins, alpha 2-6-sialoproteins and alpha 2,6-ST were markedly increased in untreated oral cancer patients compared to the controls and OPC as well as responder (CR) patients. Serum levels of the markers were higher or comparable between untreated oral cancer patients and non-responders (NR). Serum levels of alpha 2-3-sialylation were elevated in non-responders compared with the responders. Further, the observed sialylation changes in tissue and serum were found to be associated with various clinicopathological features and disease progression. Thus, the data suggest potential utility of sialylation markers in early detection, prognostication and treatment monitoring of oral cancer.

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Section: Discussionmentioning

confidence: 99%

Tissue and serum α2-3- and α2-6-linkage specific sialylation changes in oral carcinogenesis

et al. 2007

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“…expressions of α2,3-sialic acid residues of n-cadherin were altered in metastatic melanoma cell lines (10). this possibility is further supported in the experiment where soyasaponin Ⅰ significantly impaired metastatic ability of breast cancer by decreasing expression of cell surface α2,3-sialic acids (11). Inhibition expression of α2,3-sialic acid residues may decrease the migratory ability of highly metastatic B16F10 melanoma cells (12).…”

Section: Introductionmentioning

confidence: 86%

Differential expression of the α2,3-sialic acid residues in breast cancer is associated with metastatic potential

Cui

Lin²,

Yue³

et al. 2011

Oncol Rep

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Abstract. Aberrant sialylation is closely associated with the malignant phenotype of cancer cells and metastatic potential. However, the precise nature of the molecules in breast cancers has not been unveiled. In this study, we investigated the expression levels of α2,3-sialic acid residues of 50 primary tumor cases, 50 pair-matched lymph node metastasis tumor samples and in the MDA-MB-231, t-47D and McF-7 breast cancer cell lines with different metastatic potential. the expression of α2,3-sialic acid residues was analyzed by histochemistry, cytochemistry and flow cytometry with Maackia amurensis lectin (MAl). the invasion and migration abilities of cells were examined using cell adhesion and transwell in vitro assays. pair-matched lymph node metastasis tumor samples exhibited higher levels of expression of α2,3-sialic acid residues compared to that of primary tumors (p=0.0432). Furthermore, of 38 tumors cases in t1/t2 stages, 31 (81.58%) had weak staining for MAL, which specifically binds to α2,3-sialic acid residues, whereas of 12 tumor cases in t3/t4 stages, only 1 (8.33%) had weak reactions for MAl. the highly metastatic breast cancer cell line MDA-MB-231 exhibited the strongest binding to MAl and the highest expression levels of α2,3-sialic acid residues among the selected cell lines, depending on mrnA expression levels of α2,3-sialyltransferase gene. the adhesion, invasion and migration activities confirmed that MDA-MB-231 exhibited the greater cell adhesion to, migration toward and invasion to Matrigel. taken together, the high expression of α2,3-sialic acid residues in breast cancer was associated with metastatic potential. this property may be important for developing new therapeutic approaches for breast cancer.

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“…It effectively inhibits breast cancer cell MDA-MB-231 and murine melanoma cell, B16F10, from metastasis in vivo (21,22). However, soyasaponin I is difficult to be obtained in a large amount for cancer treatment.…”

Section: Introductionmentioning

confidence: 99%

A Novel Sialyltransferase Inhibitor Suppresses FAK/Paxillin Signaling and Cancer Angiogenesis and Metastasis Pathways

et al. 2011

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Increased sialyltransferase (ST) activity promotes cancer cell metastasis, and overexpression of cell surface sialic acid correlates with poor prognosis in cancer patients. To seek therapies targeting metastasis for cancer treatment, we developed a novel ST inhibitor, Lith-O-Asp, and investigated its antimetastatic and antiangiogenic effects and mechanisms. We found that cells treated with Lith-O-Asp showed a reduction of activity on various ST enzymes by in vitro and cell-based activity analyses. Lith-O-Asp inhibited migration and invasion abilities in various cancer cell lines and showed inhibitory effect on the angiogenic activity of human umbilical vein endothelial cells. Indeed, Lith-O-Asp treatment consequently delayed cancer cell metastasis in experimental and spontaneous metastasis assays in animal models. Importantly, Lith-O-Asp decreased the sialic acid modification of integrin-b1 and inhibited the expression of phospho-FAK, phospho-paxillin, and the matrix metalloprotease

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Soyasaponin-I-modified invasive behavior of cancer by changing cell surface sialic acids

Cited by 87 publications

References 32 publications

Tissue and serum α2-3- and α2-6-linkage specific sialylation changes in oral carcinogenesis

Tissue and serum α2-3- and α2-6-linkage specific sialylation changes in oral carcinogenesis

Differential expression of the α2,3-sialic acid residues in breast cancer is associated with metastatic potential

A Novel Sialyltransferase Inhibitor Suppresses FAK/Paxillin Signaling and Cancer Angiogenesis and Metastasis Pathways

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