Soyasaponin I decreases the expression of α2,3-linked sialic acid on the cell surface and suppresses the metastatic potential of B16F10 melanoma cells

Chang, Wei-Wei; Yu, Chia-Yu; Lin, Ting‐Yi; Wang, Peng-Hui; Tsai, Ying-Chieh

doi:10.1016/j.bbrc.2005.12.216

Cited by 88 publications

(76 citation statements)

References 21 publications

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“…this possibility is further supported in the experiment where soyasaponin Ⅰ significantly impaired metastatic ability of breast cancer by decreasing expression of cell surface α2,3-sialic acids (11). Inhibition expression of α2,3-sialic acid residues may decrease the migratory ability of highly metastatic B16F10 melanoma cells (12).…”

Section: Introductionmentioning

confidence: 85%

Differential expression of the α2,3-sialic acid residues in breast cancer is associated with metastatic potential

Cui

Lin²,

Yue³

et al. 2011

Oncol Rep

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Abstract. Aberrant sialylation is closely associated with the malignant phenotype of cancer cells and metastatic potential. However, the precise nature of the molecules in breast cancers has not been unveiled. In this study, we investigated the expression levels of α2,3-sialic acid residues of 50 primary tumor cases, 50 pair-matched lymph node metastasis tumor samples and in the MDA-MB-231, t-47D and McF-7 breast cancer cell lines with different metastatic potential. the expression of α2,3-sialic acid residues was analyzed by histochemistry, cytochemistry and flow cytometry with Maackia amurensis lectin (MAl). the invasion and migration abilities of cells were examined using cell adhesion and transwell in vitro assays. pair-matched lymph node metastasis tumor samples exhibited higher levels of expression of α2,3-sialic acid residues compared to that of primary tumors (p=0.0432). Furthermore, of 38 tumors cases in t1/t2 stages, 31 (81.58%) had weak staining for MAL, which specifically binds to α2,3-sialic acid residues, whereas of 12 tumor cases in t3/t4 stages, only 1 (8.33%) had weak reactions for MAl. the highly metastatic breast cancer cell line MDA-MB-231 exhibited the strongest binding to MAl and the highest expression levels of α2,3-sialic acid residues among the selected cell lines, depending on mrnA expression levels of α2,3-sialyltransferase gene. the adhesion, invasion and migration activities confirmed that MDA-MB-231 exhibited the greater cell adhesion to, migration toward and invasion to Matrigel. taken together, the high expression of α2,3-sialic acid residues in breast cancer was associated with metastatic potential. this property may be important for developing new therapeutic approaches for breast cancer.

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Section: Introductionmentioning

confidence: 85%

Differential expression of the α2,3-sialic acid residues in breast cancer is associated with metastatic potential

Cui

Lin²,

Yue³

et al. 2011

Oncol Rep

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“…Most virions and siglecs (sialic acid binding proteins) have specificity in the sialic linkage (α2-3 or α2-6) that they recognize [43,44]. During cancer metastasis, sialylation of cell receptors is also orientation specific [45][46][47]. Thus, it would not be surprising if Aβ was specific for sialic acid linkage, possibly an α2-3 linkage.…”

Section: Discussionmentioning

confidence: 99%

Development of photocrosslinked sialic acid containing polymers for use in Aβ toxicity attenuation

2008

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Abstractβ-Amyloid peptide (Aβ), the primary protein component in senile plaques associated with Alzheimer's disease (AD), has been implicated in neurotoxicity associated with AD. Previous studies have shown that the Aβ-neuronal membrane interaction plays a crucial role in Aβ toxicity. More specifically, it is thought that Aβ interacts with ganglioside rich and sialic acid rich regions of cell surfaces. In light of such evidence, we have hypothesized that the Aβ-membrane sialic acid interaction could be inhibited through use of a biomimic multivalent sialic acid compound that would compete with the cell surface for Aβ binding. To explore this hypothesis, we synthesized a series of photocrosslinked sialic acid containing oligosaccharides and tested their ability to bind Aβ and attenuate Aβ toxicity in cell culture assays. We show that a polymer prepared via the photocrosslinking of disialyllacto-N-tetraose (DSLNT) was able to attenuate Aβ toxicity at low micromolar concentrations without adversely affecting the cell viability. Polymers prepared from mono-sialyl-oligosaccharides were less effective at Aβ toxicity attenuation. These results demonstrate the feasibility of using photocrosslinked sialyl-oligosaccharides for prevention of Aβ toxicity in vitro and may provide insight into the design of new materials for use in attenuation of Aβ toxicity associated with AD.

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“…Soyasaponins are reported to be hepatoprotective (Kinjo et al 2003) and have also shown a preventive effect on the growth of human colon cancer cells (Tsai et al 2010). The chemical structure significantly influences the saponins bioactivity; thus, individual soyasaponin I exhibits lipid peroxidation inhibitory effects in vitro, antiinflammatory properties in mice with TNBS-induced colitis (Lee et al 2010), and potentially reduces metastatic ability of cancer cells (Chang et al 2006). Moreover, soyasaponins I and III are reported to induce apoptosis in cells involved in hepatocarcinoma (Zhang and Popovich 2010).…”

Section: Saponinsmentioning

confidence: 99%

Soy and Soy-Based Foods: Role in Health and Nutrition

Villares¹,

García–Lafuente²,

Palacios³

et al. 2011

Soybean and Nutrition

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Soyasaponin I decreases the expression of α2,3-linked sialic acid on the cell surface and suppresses the metastatic potential of B16F10 melanoma cells

Cited by 88 publications

References 21 publications

Differential expression of the α2,3-sialic acid residues in breast cancer is associated with metastatic potential

Differential expression of the α2,3-sialic acid residues in breast cancer is associated with metastatic potential

Development of photocrosslinked sialic acid containing polymers for use in Aβ toxicity attenuation

Soy and Soy-Based Foods: Role in Health and Nutrition

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