Soyasaponin Ag inhibits α-MSH-induced melanogenesis in B16F10 melanoma cells via the downregulation of TRP-2

Yang, Seung Hwan; Tsatsakis, Aristidis; Tzanakakis, George N.; Kim, Hong‐Suk; Sifaki, Maria; Spandidos, Demetrios Α.; Tsukamoto, Chigen; Golokhvast, Kirill S.; Izotov, Boris N.; Chung, Gyuhwa

doi:10.3892/ijmm.2017.3061

Cited by 19 publications

(16 citation statements)

References 41 publications

(44 reference statements)

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“…The intracellular melanin content was determined according to a procedure described previously [25] with minor modifications. Briefly, B16F10 cells were seeded in a 24-well plate at 1 × 10 4 cells/well and incubated for 24 h in DMEM containing 10% FBS.…”

Section: Methodsmentioning

confidence: 99%

Phlorotannins with Potential Anti-Tyrosinase and Antioxidant Activity Isolated from the Marine Seaweed Ecklonia stolonifera

et al. 2019

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Compounds were isolated from Ecklonia stolonifera Okamura, a marine brown alga widely consumed as food. Among the isolated compounds, 974-A was demonstrated for the first time to be a potent competitive inhibitor of mushroom tyrosinase activity towards l-tyrosine and l-DOPA (IC50 values = 1.57 ± 0.08 and 3.56 ± 0.22 µM, respectively). Molecular docking simulations clarified that the hydroxyl residues of the isolated compounds formed hydrogen bonds with residues at the catalytic and allosteric sites of tyrosinase, while other residues participated in hydrophobic interactions. Moreover, 974-A, phlorofucofuroeckol-A and eckol reduced the cellular melanin content and tyrosinase activity, and downregulated the expression of melanogenesis enzymes including tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2 in B16F10 melanoma cells. These compounds also effectively scavenged radicals at the cellular level. Thus, our results revealed that compounds isolated from E. stolonifera are potent tyrosinase inhibitors with potential applications in the cosmetic industry for treatment of hyperpigmentation and for the anti-browning effect in the agricultural field.

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Section: Methodsmentioning

confidence: 99%

Phlorotannins with Potential Anti-Tyrosinase and Antioxidant Activity Isolated from the Marine Seaweed Ecklonia stolonifera

et al. 2019

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“…3 B). TRP-2, one of the enzymes involved in melanin synthesis, catalyzes the reaction from DOPA chrome to 5,6-dihydroxyindole-2-carboxylic acid (DHICA) [ 22 ]. Therefore, like tyrosinase, a decrease in the protein expression level of TRP-2 is considered a cause of melanin production suppression [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

α-Mangostin suppressed melanogenesis in B16F10 murine melanoma cells through GSK3β and ERK signaling pathway

Zhou

Yotsumoto

Tian

et al. 2021

Biochemistry and Biophysics Reports

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Mangosteen (Garcinia mangostana L) fruit contains many xanthones in its pericarp, such as α-mangostin. Here, we aimed to elucidate the physiological effect of α-mangostin and the mechanism on melanogenesis in mouse B16F10 cells. The melanin production in B16F10 cells was decreased by α-mangostin treatment. α-Mangostin also suppressed the enzymatic activity of tyrosinase, the critical enzyme for melanin synthesis. Furthermore, Western blot analysis revealed that α-mangostin down-regulated the protein quantity of tyrosinase, tyrosinase relative protein (TRP)-2, and microphthalmia-associated transcription factor (MITF). We also used inhibitors of the extracellular signal-regulated kinase (ERK), and glycogen synthase kinase 3 (GSK-3β) to identify the upstream signaling cascade of MITF. Results showed us GSK3β plays a more important role in α-mangostin regulated melanogenesis. Further, the de-pigmentation effect on normal human epidermal melanocytes (NHEMs) of α-mangostin was also confirmed. These results suggested that α-mangostin is a reagent for depigmentation and it has the potential to be applied as a component of cosmetics or pharmaceuticals for the therapy of spots, chloasma, or melanosis.

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“…The intracellular melanin content was determined using a slightly modified procedure described elsewhere [ 38 ]. Briefly, B16F10 cells were seeded in a 24-well plate at 2 × 10 4 cells/well and incubated for 24 h in DMEM containing 10% FBS.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant Compounds, Kirenol and Methyl ent-16α, 17-dihydroxy-kauran-19-oate Bioactivity-Guided Isolated from Siegesbeckia glabrescens Attenuates MITF-Mediated Melanogenesis via Inhibition of Intracellular ROS Production

Shim

Lee

2021

Molecules

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Siegesbeckia glabrescens (Compositae), an annual herb indigenous to Korean mountainous regions and has been eaten as a food in Korea. This study investigated ABTS, DPPH and nitric oxide (NO) radical-scavenging activities, and melanin production and TYR inhibitory effects-guided fractionation to identify therapeutic phytochemicals from S. glabrescens that can attenuate oxidation and melanogenesis in murine melanoma B16F10 cells. Nine compounds with inhibitory effects on melanin production, and TYR activity, and ABTS, DPPH, and NO radical scavenging activity were isolated from the 100% ethanol fraction from S. glabrescens. Among the nine compounds, kirenol (K), methyl ent-16α, 17-dihydroxy-kauran-19-oate (MDK) had strong inhibitory effects on melanin production and TYR activity with antioxidant effects. Western blot analysis revealed that K and MDK suppressed tyrosinase-related protein (TYRP)-1, TYRP-2 and microphthalmia-associated transcription factor (MITF) expression. Moreover, these two compounds inhibited intracellular reactive oxygen species (ROS) level in tert-butyl hydroperoxide (t-BHP)-treated B16F10 cells. Our results suggest that S. glabrescens containing active compounds such as K and MDK, which has antioxidant and antimelanogenesis effects, is the potent therapeutic and functional material for the prevention of oxidation-induced hyperpigmentation.

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Soyasaponin Ag inhibits α-MSH-induced melanogenesis in B16F10 melanoma cells via the downregulation of TRP-2

Cited by 19 publications

References 41 publications

Phlorotannins with Potential Anti-Tyrosinase and Antioxidant Activity Isolated from the Marine Seaweed Ecklonia stolonifera

Phlorotannins with Potential Anti-Tyrosinase and Antioxidant Activity Isolated from the Marine Seaweed Ecklonia stolonifera

α-Mangostin suppressed melanogenesis in B16F10 murine melanoma cells through GSK3β and ERK signaling pathway

Antioxidant Compounds, Kirenol and Methyl ent-16α, 17-dihydroxy-kauran-19-oate Bioactivity-Guided Isolated from Siegesbeckia glabrescens Attenuates MITF-Mediated Melanogenesis via Inhibition of Intracellular ROS Production

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