Soy Protein Extract (SPE) Exhibits Differential In Vitro Cell Proliferation Effects in Oral Cancer and Normal Cell Lines

Kingsley, Karl; Truong, Khanh; Löw, E C Von; Hill, Charles; Chokshi, Shruti B; Phipps, Don; West, Matthew; Keiserman, Mark A.; Bergman, Christine J.

doi:10.3109/19390211.2011.571656

Cited by 13 publications

(11 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Soy isoflavones have been reported to exert a cytotoxic effect on cancer cells 187 and can be used as dietary modulators for the treatment of cancer and other diseases. Increased DNA-repair enzyme activity has been observed in lung cancer cells, and complementary treatment with soy isoflavones rendered these cells more sensitive to radiation therapy.…”

Section: Importance Of Phytochemicals In Modulating the Functions Of mentioning

confidence: 99%

See 1 more Smart Citation

APE1/Ref-1 as an emerging therapeutic target for various human diseases: phytochemical modulation of its functions

et al. 2014

View full text Add to dashboard Cite

Apurinic/apyrimidinic endonuclease 1 (APE1) is a multifunctional enzyme involved in the base excision repair (BER) pathway, which repairs oxidative base damage caused by endogenous and exogenous agents. APE1 acts as a reductive activator of many transcription factors (TFs) and has also been named redox effector factor 1, Ref-1. For example, APE1 activates activator protein-1, nuclear factor kappa B, hypoxia-inducible factor 1α, paired box gene 8, signal transducer activator of transcription 3 and p53, which are involved in apoptosis, inflammation, angiogenesis and survival pathways. APE1/Ref-1 maintains cellular homeostasis (redox) via the activation of TFs that regulate various physiological processes and that crosstalk with redox balancing agents (for example, thioredoxin, catalase and superoxide dismutase) by controlling levels of reactive oxygen and nitrogen species. The efficiency of APE1/Ref-1's function(s) depends on pairwise interaction with participant protein(s), the functions regulated by APE1/Ref-1 include the BER pathway, TFs, energy metabolism, cytoskeletal elements and stress-dependent responses. Thus, APE1/Ref-1 acts as a ‘hub-protein' that controls pathways that are important for cell survival. In this review, we will discuss APE1/Ref-1's versatile nature in various human etiologies, including neurodegeneration, cancer, cardiovascular and other diseases that have been linked with alterations in the expression, subcellular localization and activities of APE/Ref-1. APE1/Ref-1 can be targeted for therapeutic intervention using natural plant products that modulate the expression and functions of APE1/Ref-1. In addition, studies focusing on translational applications based on APE1/Ref-1-mediated therapeutic interventions are discussed.

show abstract

Section: Importance Of Phytochemicals In Modulating the Functions Of mentioning

confidence: 99%

“…In addition, APE1/Ref-1 downregulation by soy extracts rendered pancreatic and oral cancer cells more responsive toward radiotherapy. 134, 187 …”

Section: Importance Of Phytochemicals In Modulating the Functions Of mentioning

confidence: 99%

APE1/Ref-1 as an emerging therapeutic target for various human diseases: phytochemical modulation of its functions

et al. 2014

View full text Add to dashboard Cite

show abstract

“…1,2,3,4,5 In recent years, much research has been conducted on genistein, an isoflavone that has been proposed to have anticancer activity. 6,7,8 The potential chemopreventive effect of genistein has prompted researchers to investigate soybean further for anticancer agents.…”

Section: Introductionmentioning

confidence: 99%

Screening and Identification of Glyceollins and Their Metabolites by Electrospray Ionization Tandem Mass Spectrometry with Precursor Ion Scanning

et al. 2013

View full text Add to dashboard Cite

A method has been developed for screening glyceollins and their metabolites based upon precursor ion scanning. Under higher-energy collision conditions employing a triple quadrupole mass spectrometer in the negative ion mode, deprotonated glyceollin precursors yield a diagnostic radical product ion at m/z 148. We propose this resonance-stabilized radical anion, formed in violation of the even-electron rule, to be diagnostic of glyceollins and glyceollin metabolites. Liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) established that scanning for precursors of m/z 148 can identify glyceollins and their metabolites from plasma samples originating from rats dosed with glyceollins. Precursor peaks of interest were found at m/z 337, 353, 355, 417, and 433. The peak at m/z 337 corresponds to deprotonated glyceollins, whereas the others represent metabolites of glyceollins. Accurate mass measurement confirmed m/z 417 to be a sulfated metabolite of glyceollins. The peak at m/z 433 is also sulfated, but it contains an additional oxygen, as confirmed by accurate mass measurement. The latter metabolite differs from the former likely by the replacement of a hydrogen with a hydroxyl moiety. The peaks at m/z 353 and 355 are proposed to correspond to hydroxylated metabolites of glyceollins wherein the latter additionally undergoes a double bond reduction.

show abstract

“…Kingsley et al have shown that the adhesion of cells of OSCC (CAL 27 and SCC25 cell lines) was clearly decreased when cells were treated with soy protein extract 45 belonging to different isoflavones, including genistein. 46 …”

Section: Discussionmentioning

confidence: 99%

In vitro study on anti-cancer properties of genistein in tongue cancer

Ardito¹,

Pellegrino²,

Perrone³

et al. 2017

OTT

View full text Add to dashboard Cite

PurposeTongue cancer is an extremely aggressive disease and is characterized by a poor prognosis. It is a complex disease to treat and current therapies have produced mediocre results with many side effects. Some facts suggest that natural essences can support traditional cancer therapy by carrying out a synergistic function with chemotherapy. Therefore, we evaluated the antitumor effects of genistein on tongue carcinoma cells.MethodsGenistein 20, 50 and 100 µM were used for 24, 48 and 72 hours on 3 tongue carcinoma cell lines. xCELLigence system was used to evaluate the effects on cell adhesion, proliferation and to calculate IC50 values. Both MTT assay and Trypan blue assay were used to evaluate alterations in cell viability, scratch assay for cell migration and Western blot analysis for expression of some proteins.ResultsCell adhesion was inhibited especially between 20 and 50 µM of genistein treatment. Proliferation was reduced by 50% for treatments with 20 µM at 24 hours, with 20 or 50 µM at 48 and 50 µM at 72 hours (P<0.0001). Viability tests confirmed a proportional reduction in concentration of genistein and duration of treatments. Even cell migration was reduced significantly (P<0.001). Genistein down-regulates vitronectin, OCT4 and survivin.ConclusionThis in vitro study clarifies the anti-tumor effect of genistein on tongue carcinoma. In vivo studies are needed to confirm these data and develop a suitable delivery system that is capable of acting directly on tumor.

show abstract

Soy Protein Extract (SPE) Exhibits Differential In Vitro Cell Proliferation Effects in Oral Cancer and Normal Cell Lines

Cited by 13 publications

References 58 publications

APE1/Ref-1 as an emerging therapeutic target for various human diseases: phytochemical modulation of its functions

APE1/Ref-1 as an emerging therapeutic target for various human diseases: phytochemical modulation of its functions

Screening and Identification of Glyceollins and Their Metabolites by Electrospray Ionization Tandem Mass Spectrometry with Precursor Ion Scanning

In vitro study on anti-cancer properties of genistein in tongue cancer

Contact Info

Product

Resources

About