Sox9+ cells are required for salivary gland regeneration after radiation damage via the Wnt/β-catenin pathway

Xu, Xiuyun; Xiong, Guanglei; Zhang, Ming; Xie, Jiaxiang; Chen, Shuang; Li, Kang; Li, Jingting; Bao, Yongzhan; Wang, Cheng; Chen, Demeng

doi:10.1016/j.jgg.2021.09.008

Cited by 12 publications

(8 citation statements)

References 28 publications

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“…The Tomato+ organoids were observed to be distributed in both the acinar and ductal compartments of the SMGs. Furthermore, the salivary flow rate in mice that received organoid transplants was higher than nontreated mice, suggesting the potential of bioengineered SG organoids in replacing or regenerating functionally relevant SG structures . Collectively, these studies provide reassurance regarding the potential of PSC- and ASC-derived SG microtissues, with supporting mesenchyme, to facilitate SG regeneration after injury.…”

Section: Translating the Bioengineered Salivary Glandscurrent Applic...mentioning

confidence: 85%

“…Furthermore, the salivary flow rate in mice that received organoid transplants was higher than nontreated mice, suggesting the potential of bioengineered SG organoids in replacing or regenerating functionally relevant SG structures. 126 Collectively, these studies provide reassurance regarding the potential of PSC-and ASC-derived SG microtissues, with supporting mesenchyme, to facilitate SG regeneration after injury. Still, thus far, only a handful of studies have successfully shown SG organoid or spheroid transplantation in the in vivo models.…”

Section: Translating the Bioengineered Salivary Glands�current Applic...mentioning

confidence: 87%

“…125 Leveraging the self-renewal capacity and lineage specificity of SG tissueresident stem/progenitor cells, Xu et al employed organoids derived from Sox9+ cells to rescue radiation-injured SGs. 126 Around 200 organoids sourced from Tomato+ mice SMGs were injected into the irradiated SMGs of wild-type mice. The Tomato+ organoids were observed to be distributed in both the acinar and ductal compartments of the SMGs.…”

Section: Translating the Bioengineered Salivary Glands�current Applic...mentioning

confidence: 99%

“…The study reported that BMMSCs could be utilized as an alternative and ample source to generate bioengineered SGs for use in transplantation . Leveraging the self-renewal capacity and lineage specificity of SG tissue-resident stem/progenitor cells, Xu et al employed organoids derived from Sox9+ cells to rescue radiation-injured SGs . Around 200 organoids sourced from Tomato+ mice SMGs were injected into the irradiated SMGs of wild-type mice.…”

Section: Translating the Bioengineered Salivary Glandscurrent Applic...mentioning

confidence: 99%

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Bioengineered Salivary Gland Microtissues─A Review of 3D Cellular Models and their Applications

Pillai,

Munguia-Lopez,

Tran

2024

ACS Appl. Bio Mater.

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Salivary glands (SGs) play a vital role in maintaining oral health through the production and release of saliva. Injury to SGs can lead to gland hypofunction and a decrease in saliva secretion manifesting as xerostomia. While symptomatic treatments for xerostomia exist, effective permanent solutions are still lacking, emphasizing the need for innovative approaches. Significant progress has been made in the field of three-dimensional (3D) SG bioengineering for applications in gland regeneration. This has been achieved through a major focus on cell culture techniques, including soluble cues and biomaterial components of the 3D niche. Cells derived from both adult and embryonic SGs have highlighted key in vitro characteristics of SG 3D models. While still in its first decade of exploration, SG spheroids and organoids have so far served as crucial tools to study SG pathophysiology. This review, based on a literature search over the past decade, covers the importance of SG cell types in the realm of their isolation, sourcing, and culture conditions that modulate the 3D microenvironment. We discuss different biomaterials employed for SG culture and the current advances made in bioengineering SG models using them. The success of these 3D cellular models are further evaluated in the context of their applications in organ transplantation and in vitro disease modeling.

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Section: Translating the Bioengineered Salivary Glandscurrent Applic...mentioning

confidence: 85%

Section: Translating the Bioengineered Salivary Glands�current Applic...mentioning

confidence: 87%

Section: Translating the Bioengineered Salivary Glands�current Applic...mentioning

confidence: 99%

Section: Translating the Bioengineered Salivary Glandscurrent Applic...mentioning

confidence: 99%

See 2 more Smart Citations

Bioengineered Salivary Gland Microtissues─A Review of 3D Cellular Models and their Applications

Pillai,

Munguia-Lopez,

Tran

2024

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

show abstract

“… 46 Moreover, SOX9 was previously shown to regulate stem cell properties in the SG and play an essential regulatory role in SG development and regeneration. 27 , 47 In this study, we validated the role of SOX9 by exploring the publicly available RNA-seq data from a breast cancer epithelial cell line with SOX9 knockdown. Previous cross-tissue studies using single-cell gene expression data provided evidence that various epithelial cells exhibit common features including both salivary and mammary glands.…”

Section: Discussionmentioning

confidence: 99%