Sox2 and Pou2f1 interact to control lens and olfactory placode development

Donner, Amy; Episkopou, Vasso; Maas, Richard L.

doi:10.1016/j.ydbio.2006.10.047

Cited by 107 publications

(85 citation statements)

References 64 publications

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“…At E10.5, Foxg1 is expressed throughout the invaginating olfactory placode in a pattern similar to a putative stem cell marker, Sox2 (Fig. 1 A) (Donner et al, 2007). We next localized Foxg1 protein by immunohistochemistry, which revealed two important findings.…”

Section: Foxg1 Is Expressed In Early Precursors Of the Olfactory Lineagementioning

confidence: 89%

Foxg1Is Required for Development of the Vertebrate Olfactory System

Duggan

DeMaria²,

Baudhuin³

et al. 2008

J. Neurosci.

107

102

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Illuminating the molecular identity and regulation of early progenitor cells in the olfactory sensory epithelium represents an important challenge in the field of neural development. We show in both mouse and zebrafish that the winged helix transcription factor Foxg1 is expressed in an early progenitor population of the olfactory placode. In the mouse, Foxg1 is first expressed throughout the olfactory placode but later becomes restricted to the ventrolateral olfactory epithelium. The essential role of Foxg1 in olfactory development is demonstrated by the strikingly severe phenotype of Foxg1 knock-out mice: older embryos have no recognizable olfactory structures, including epithelium, bulb, or vomeronasal organs. Initially, a small number of olfactory progenitors are specified but show defects in both proliferation and differentiation. Similarly, antisense RNA knockdown of Foxg1 expression in the zebrafish shows a reduction in the number of neurons and mitotic cells in olfactory rosettes, mirroring the phenotype seen in the mouse Foxg1 null mutant. Using mosaic analysis in the zebrafish, we show that Foxg1 is required cell-autonomously for the production of mature olfactory receptor neurons. Therefore, we identified an evolutionarily conserved requirement for Foxg1 in the development of the vertebrate olfactory system.

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Section: Foxg1 Is Expressed In Early Precursors Of the Olfactory Lineagementioning

confidence: 89%

Foxg1Is Required for Development of the Vertebrate Olfactory System

Duggan

DeMaria²,

Baudhuin³

et al. 2008

J. Neurosci.

107

102

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“…There are examples that these factors interact to coregulate genes outside ES cells. POU2F1 and SOX2 have been shown to function synergistically in the regulation of the PAX6 and nestin genes later in neural development (Donner et al 2007;Jin et al 2009). SOX2 is widely expressed and therefore present with POU2F1 in many more tissues than POU5F1, including cell types and cell lines (e.g., HeLa) where POU2F1 is the only POU factor detectable (M Gemberling and L Ferraris, unpubl.).…”

Section: Discussionmentioning

confidence: 99%

Combinatorial binding of transcription factors in the pluripotency control regions of the genome

Ferraris¹,

Stewart²,

Kang³

et al. 2011

Genome Res.

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The pluripotency control regions (PluCRs) are defined as genomic regions that are bound by POU5F1, SOX2, and NANOG in vivo. We utilized a high-throughput binding assay to record more than 270,000 different DNA/protein binding measurements along incrementally tiled windows of DNA within these PluCRs. This high-resolution binding map is then used to systematically define the context of POU factor binding, and reveals patterns of cooperativity and competition in the pluripotency network. The most prominent pattern is a pervasive binding competition between POU5F1 and the forkhead transcription factors. Like many transcription factors, POU5F1 is co-expressed with a paralog, POU2F1, that shares an apparently identical binding specificity. By analyzing thousands of binding measurements, we discover context effects that discriminate POU2F1 from POU5F1 binding. Proximal NANOG binding promotes POU5F1 binding, whereas nearby SOX2 binding favors POU2F1. We demonstrate by cross-species comparison and by chromatin immunoprecipitation (ChIP) that the contextual sequence determinants learned in vitro are sufficient to predict POU2F1 binding in vivo.

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“…The ectodermal enhancer (EE) was shown to direct Pax6 expression in the surface ectoderm, lens and cornea (Kammandel et al, 1999;Williams et al, 1998). The EE contains binding sites for the Meis (Zhang et al, 2002), Sox (Aota et al, 2003;Donner et al, 2006a), Six3 and Oct family of proteins (Donner et al, 2006a), as well as a single Pax6-binding site (Aota et al, 2003). Transgenic expression supported by this enhancer is initiated in the surface ectoderm at E8.75 in mouse, i.e.…”

Section: Regulation Of Pax6 Gene Expression In Lens-thementioning

confidence: 99%

Genetic and epigenetic mechanisms of gene regulation during lens development

Cvekl

Duncan

2007

Progress in Retinal and Eye Research

139

124

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Recent studies demonstrated a number of links between chromatin structure, gene expression, extracellular signaling and cellular differentiation during lens development. Lens progenitor cells originate from a pool of common progenitor cells, the pre-placodal region (PPR) which is formed due to a complex exchange of extracellular signals between the neural plate, naïve ectoderm and mesendoderm. A specific commitment to the lens program over alternate choices such as the formation of olfactory epithelium or the anterior pituitary is manifested by the formation of a thickened surface ectoderm, the lens placode. Mouse lens progenitor cells are characterized by the expression of a complement of lens lineage-specific transcription factors including Pax6, Six3 and Sox2, controlled by FGF and BMP signaling, followed later by c-Maf, Mab21like1, Prox1 and FoxE3. Proliferation of lens progenitors together with their morphogenetic movements results in the formation of the lens vesicle. This transient structure, comprised of lens precursor cells, is polarized with its anterior cells retaining their epithelial morphology and proliferative capacity, whereas the posterior lens precursor cells initiate terminal differentiation forming the primary lens fibers. Lens differentiation is marked by expression and accumulation of crystallins and other structural proteins. The transcriptional control of crystallin genes is characterized by the reiterative use of transcription factors required for the establishment of lens precursors in combination with more ubiquitously expressed factors (e.g. AP-1, AP-2α, CREB and USF) and recruitment of histone acetyltransferases (HATs) CBP and p300, and chromatin remodeling complexes SWI/SNF and ISWI. These studies have poised the study of lens development at the forefront of efforts to understand the connections between development, cell signaling, gene transcription and chromatin remodeling.

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Sox2 and Pou2f1 interact to control lens and olfactory placode development

Cited by 107 publications

References 64 publications

Foxg1Is Required for Development of the Vertebrate Olfactory System

Foxg1Is Required for Development of the Vertebrate Olfactory System

Combinatorial binding of transcription factors in the pluripotency control regions of the genome

Genetic and epigenetic mechanisms of gene regulation during lens development

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