Sox Gene Family Revealed Genetic Variations in Autotetraploid Carassius auratus

Huang, Xu; Wu, Chang; Gong, Kaijun; Chen, Qian; Gu, Qianhong; Qin, Huan; Zhang, Chun; Yu, Tingting; Li, Yang; Fu, Wen; Wang, Yude; Qin, Qinbo; Liu, Shaojun

doi:10.3389/fgene.2020.00804

Cited by 5 publications

(1 citation statement)

References 57 publications

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“…Genes can undergo an increase in copy number after polyploidy [23]. Several studies analyzed the Sox gene family of natural and arti cial polyploid sh and found that most Sox genes increased copy number after polyploidy events [24]. After the number of gene copies increases, some may undergo pseudogenization, neofunctionalization, or subfunctionalization due to the pressures of natural evolution and selection [25].…”

Section: Discussionmentioning

confidence: 99%

Expression and localization of HPG axis-related genes in Carassius auratus with different ploidy

Yang

Deng

et al. 2023

Preprint

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Background: In the Dongting water system, the Carassius auratus (Crucian carp) complex is characterized by the coexistence of diploid forms (2n=100, 2nCC) and polyploid forms. The diploid (2nCC) and triploid C. auratus (3n=150, 3nCC) had the same fertility levels, reaching sexual maturity at one year. Results: The nucleotide sequence, gene expression, methylation, and immunofluorescence of the gonadotropin releasing hormone 2(Gnrh2), Gonadotropin hormone beta(Gthβ),and Gonadotropin-releasing hormone receptor(Gthr) genes pivotal genes of the hypothalamic-pituitary-gonadal (HPG) axis were analyzed.The analysis results indicated that Gnrh2, follicle stimulating hormone receptor(Fshr), and Lethal hybrid rescue(Lhr) genes increased the copy number and distinct structural differentiation in 3nCC compared to that in 2nCC. The expression levels of HPG axis genes in 3nCC were higher than 2nCC (P<0.05), which can promote the production and secretion of sex steroid hormones conducive to the gonadal development of 3nCC. Meanwhile, the DNA methylation levels in the promoter regions of the HPG axis genes were lower in 3nCC than in 2nCC. These results suggested that methylation of the promoter region had a potential regulatory effect on gene structure and expression after triploidization. Immunofluorescence showed that the localization of the Fshβ, Lhβ, and Fshr genes between 3nCC and 2nCC remained unchanged, ensuring the normal expression of these genes at the corresponding sites after triploidization. Conclusions: Relevant research results provide cell and molecular biology evidence for normal reproductive activities such as gonad development and gamete maturation in triploid C. auratus, and contribute to further understanding of the genetic basis for fertility restoration in triploid C. auratus

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Section: Discussionmentioning

confidence: 99%

Expression and localization of HPG axis-related genes in Carassius auratus with different ploidy

Yang

Deng

et al. 2023

Preprint

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Molecular Characterization, Phylogeny, and Expression Profiles of SoxE Subfamily in Scophthalmus maximus

Ji,

Guo,

Shi

et al. 2024

Journal of Applied Ichthyology

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SOXE transcription factors, including SOX8, SOX9, and SOX10, regulate several developmental events, such as sex determination, chondrogenesis, and neurogenesis. This study systematically identified six SoxE subfamily genes from the turbot (Scophthalmus maximus) genome and transcriptome, including SmSox8a, SmSox8b, SmSox9a, SmSox9b, SmSox10a, and SmSox10b. The duplicates of three SOXE members revealed that SoxE subfamily genes in the turbot underwent significant expansion. Relatively conserved exon-intron structures and intron insertions were detected using genomic structural analysis. Besides, phylogenetic analysis supported the previous classification of the SoxE subfamily. Results of the expression profile revealed that turbot SoxE subfamily genes may be involved in different activities, such as neurogenesis and gonad development. These findings would assist in the understanding of fish SoxE gene subfamily activities and evolution.

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Expression and localization of HPG axis-related genes in Carassius auratus with different ploidy

Xu,

Yang,

Deng

et al. 2024

Front. Endocrinol.

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IntroductionIn the Dongting water system, the Carassius auratus (Crucian carp) complex is characterized by the coexistence of diploid forms (2n=100, 2nCC) and polyploidy forms. The diploid (2nCC) and triploid C.auratus (3n=150, 3nCC) had the same fertility levels, reaching sexual maturity at one year. MethodsThe nucleotide sequence, gene expression, methylation, and immunofluorescence of the gonadotropin releasing hormone 2(Gnrh2), Gonadotropin hormone beta(Gthβ), and Gonadotropin-releasing hormone receptor(Gthr) genes pivotal genes of the hypothalamic-pituitary-gonadal (HPG) axis were analyzed. ResultsThe analysis results indicated that Gnrh2, follicle-stimulating hormone receptor(Fshr), and Lethal hybrid rescue(Lhr) genes increased the copy number and distinct structural differentiation in 3nCC compared to that in 2nCC. The transcript levels of HPG axis genes in 3nCC were higher than 2nCC (P<0.05), which could promote the production and secretion of sex steroid hormones conducive to the gonadal development of 3nCC. Meanwhile, the DNA methylation levels in the promoter regions of the HPG axis genes were lower in 3nCC than in 2nCC. These results suggested that methylation of the promoter region had a potential regulatory effect on gene expression after triploidization. Immunofluorescence showed that the localization of the Fshβ, Lhβ, and Fshr genes between 3nCC and 2nCC remained unchanged, ensuring the normal expression of these genes at the corresponding sites after triploidization. DiscussionRelevant research results provide cell and molecular biology evidence for normal reproductive activities such as gonad development and gamete maturation in triploid C. auratus, and contribute to further understanding of the genetic basis for fertility restoration in triploid C. auratus.

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Sox Gene Family Revealed Genetic Variations in Autotetraploid Carassius auratus

Cited by 5 publications

References 57 publications

Expression and localization of HPG axis-related genes in Carassius auratus with different ploidy

Expression and localization of HPG axis-related genes in Carassius auratus with different ploidy

Molecular Characterization, Phylogeny, and Expression Profiles of SoxE Subfamily in Scophthalmus maximus

Expression and localization of HPG axis-related genes in Carassius auratus with different ploidy

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