Sources of Error in Glucose Determinations in Neonatal Blood by Glucose Oxidase Methods, Including Dextrostix

Fox, Rosemary E.; Redstone, D.

doi:10.1093/ajcp/66.4.658

Cited by 12 publications

(4 citation statements)

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“…EML 105 readings overestimated plasma glucose throughout the tested range, which is surprising, as one expects whole blood glucose to be on average 10-15% lower (16), with some experimental work quoting variations ranging between 4 and 47% (17,18). This is explained by the space occupied by blood solids (plasma proteins, haemoglobin and cell membranes), the lower water content of red blood cells compared to an equivalent volume of plasma (approximately 71% and 93%, respectively) and, nally, interference with glucose oxidase-peroxidase-based strip methods by bilirubin, uric acid, ascorbic acid and haemolysis (glutathione release) (18,19). In addition, as neither method of analysis involves red blood cell lysis, a small residue of glucose remains within the intracellular space at equilibrium.…”

Section: Discussionmentioning

confidence: 99%

Comparison of EML 105 and Advantage analysers measuring capillary versus venous whole blood glucose in neonates

McNamara

Sharief

2001

Acta Paediatrica

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McNamara PJ, Sharief N. Comparison of EML 105 and Advantage analysers measuring capillary versus venous whole blood glucose in neonates. Acta Paediatr 2001; 90: 1033-1041. Stockholm. ISSN 0803-5253Aim: Near-patient blood glucose monitoring is an essential component of neonatal intensive care but the analysers currently used are unreliable and inaccurate. The aim of this study was to compare a new glucose electrode-based analyser (EML 105) and a non-wipe re ectance photometry method (Advantage) as opposed to a recognized laboratory reference method (Hexokinase). We also investigated the effect of sample route and haematocrit on the accuracy of the glucose readings obtained by each method of analysis. Methods: Whole blood glucose concentrations ranging from 0 to 3.5 mmol/l were carefully prepared in a laboratory setting and blood samples from each respective solution were then measured by EML 105 and Advantage analysers. The results obtained were then compared with the corresponding plasma glucose reading obtained by the Hexokinase method, using linear regression analysis. An in vivo study was subsequently performed on 103 neonates, over a 1-y period, using capillary and venous whole blood samples. Whole blood glucose concentration was estimated from each sample using both analysers and compared with the corresponding plasma glucose concentration estimated by the Hexokinase method. Venous blood was centrifuged and haematocrit was estimated using standardized curves. The effect of haematocrit on the agreement between whole blood and plasma glucose was investigated, estimating the degree of correlation on a scatterplot of the results and linear regression analysis. Results: Both the EML 105 and Hexokinase methods were highly accurate, in vitro, with small proportiona l biases of 2% and 5%, respectively. However, in vivo, both study analysers overestimated neonatal plasma glucose, ranging from at best 0.45 mmol/l (EML 105 venous) to 0.69 mmol/l (EML capillary). There was no signi cant difference in the agreement of capillary (GD = 0.12, 95% CI. {¡0.32, 0.08}, p = 0.2) or venous samples (GD = 0.05, 95% CI. {0.09, 0.19}, p = 0.49) with plasma glucose when analysed by either study method (GD = glucose difference between study analyser and reference method) However, the venous samples analysed by EML 105 estimated plasma glucose signi cantly better than capillary samples using the same method of analysis (GD = 0.24, 95% CI. {0.09, 0.38}, p < 0.01). The relationship between haematocrit and the resultant glucose differences was non-linear with correlation coef cients of r = ¡0.057 (EML 105 capillary), r = 0.145 (EML 105 venous), r = ¡0.127 (Advantage capillary) and r = ¡0.275 (Advantage venous). There was no signi cant difference in the effect of haematocrit on the performance of EML 105 versus Advantage, regardless of the sample route.Conclusion: Both EML 105 and Advantage overestimated plasma glucose, with no signi cant difference in the performance of either analyser, regardless of the route of analysis. Agreement with p...

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Section: Discussionmentioning

confidence: 99%

Comparison of EML 105 and Advantage analysers measuring capillary versus venous whole blood glucose in neonates

McNamara

Sharief

2001

Acta Paediatrica

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“…However, techniques based on enzymes require many samples and reagents as well as calibration controls 8. In some cases, accurate measurement is impossible without deproteination and other complicated procedures 9. In addition, in the case of patients undergoing intravenous infusion, overestimated results may be obtained because of reducing sugars such as lactose and galactose that are included in the IV solution 10, 11.…”

Section: Application Of Microchip Electrophoresis To Biomarker Anamentioning

confidence: 99%

Application of microchip electrophoresis for clinical tests

Yatsushiro

Kataoka

2011

Electrical Engineering Japan

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SUMMARYMicrochip electrophoresis has recently attracted much attention in the field of nuclear acid analysis due to its high efficiency, ease of operation, low consumption of samples and reagents, and relatively low costs. In addition, the analysis has expanded to analytical fields, including the analysis not only of DNA but also of RNA, proteins, sugar chains, cellular functions, etc. In this paper we describe the creation of high-performance monitoring systems for human blood glucose levels and α-amylase activity in human plasma using microchip electrophoresis.

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“…These conventional enzymatic methods require frequent calibration controls and reagents, and the whole process is very costly [15]. A glucose oxidase method for plasma glucose without protein precipitation gives low results for neonatal blood [16]. Furthermore, in the presence of galactose, maltose, lactose, or mannose in the blood, particularly in subjects receiving intravenous drip injection containing these carbohydrates, blood glucose concentrations are overestimated compared to accurate concentrations determined using glucose dehydrogenase [17,18].…”

Section: Introductionmentioning

confidence: 99%

Determination of human blood glucose levels using microchip electrophoresis

et al. 2007

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Determination of human blood glucose levels using microchip electrophoresisA high-performance monitoring system for human blood glucose levels was developed using microchip electrophoresis with a plastic chip. The combination of reductive amination as glucose labeling with fluorescent 2-aminoacridone (AMAC) and glucose-borate complex formation realized the highly selective detection of glucose even in a complex matrix such as a blood sample. The migration time of a single peak, observed on an electropherogram of AMAC-labeled plasma, closely resembled that of glucose standard solution. The treatment of plasma with hexokinase or glucokinase for glucose phosphorylation resulted in a peak shift from approximately 145 to 70 s, corresponding to glucose and glucose-6-phosphate, respectively. A double-logarithm plot revealed a linear relationship between glucose concentration and fluorescence intensity in the range of 1-300 mM of glucose (r 2 = 0.9963; p ,0.01), and the detection limit was 0.92 mM. Furthermore, blood glucose concentrations estimated from the standard curves of three subjects were compared with results obtained by conventional colorimetric analysis using glucose dehydrogenase. Good correlation was observed between methods according to simple linear regression analysis (p ,0.05). The reproducibility of the assay was about 6.3-9.1% (RSD) and the within-days and between-days reproducibility were 1.6-8.4 and 5.2-7.2%, respectively. This system enables us to determine blood glucose with high sensitivity and accuracy, and will be applicable to clinical diagnosis.

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Sources of Error in Glucose Determinations in Neonatal Blood by Glucose Oxidase Methods, Including Dextrostix

Cited by 12 publications

References 0 publications

Comparison of EML 105 and Advantage analysers measuring capillary versus venous whole blood glucose in neonates

Comparison of EML 105 and Advantage analysers measuring capillary versus venous whole blood glucose in neonates

Application of microchip electrophoresis for clinical tests

Determination of human blood glucose levels using microchip electrophoresis

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