Source tracking of antibiotic resistance genes in the environment — Challenges, progress, and prospects

Li, Li-Guan; Huang, Qi; Yin, Xiaole; Zhang, Tong

doi:10.1016/j.watres.2020.116127

Cited by 88 publications

(63 citation statements)

References 119 publications

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“…As a new aquaculture model, it is necessary to evaluate the safety and environmental impact of biofloc-based aquaculture models by analyzing the ARGs within them. The successful application of metagenomics in environmental pollution tracking paves the way for the establishment of a new accurate source tracking framework, which greatly simplifies the ARGs variant-based source tracking process (Li et al, 2020). The relative abundance of total ARGs in aquaculture systems based on biofloc was about 2.46-5.82 × 10 −4 ppm, which was similar to that of coastal industrial mariculture (0.27-4.55 × 10 −4 ppm) (Wang et al, 2018).…”

Section: Discussionmentioning

confidence: 82%

Metagenomic Analysis of Bacterial Communities and Antibiotic Resistance Genes in Penaeus monodon Biofloc-Based Aquaculture Environments

Chen

Zhao

et al. 2022

Front. Mar. Sci.

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Biofloc technology (BFT) is one of the most promising technologies in global aquaculture for the purpose of improving water quality, waste treatment, and disease prevention in intensive aquaculture systems. However, characterization of the microbial species and antibiotic resistance potentially present in biofloc-based aquaculture environments is needed. In this study, we used high-throughput sequencing technology to comprehensively compare the bacterial communities in mariculture ponds of Penaeus monodon (P. monodon), by testing of water, biofloc, and intestine of P. monodon. Operational taxonomic units (OTUs) cluster analysis showed that the nine samples tested divided into 45 phyla and 457 genera. Proteobacteria was the dominant bacteria in water, biofloc and prawn intestine. In biofloc and intestine, the Ruegeria (2.23–6.31%) genus represented the largest proportion of bacteria, with Marivita (14.01–20.94%) the largest group in water. Microbial functional annotation revealed that in all the samples, genes encoding metabolism were predominant. The antibiotic resistance gene annotation showed the highest absolute abundance of patB, adeF, OXA-243, and Brucella_suis_mprF from Proteobacteria. PatB (11.33–15.01%), adeF (15.79–18.16%), OXA-243 (35.65%), and Brucella_suis_mprF (10.03%) showed the highest absolute abundance of antibiotic resistance genes in water, biofloc, and intestines, respectively. These findings may greatly increase our understanding of the characteristics of the microbiota of shrimp biofloc-based aquaculture systems and the complex interactions among shrimp, ambient microflora, and environmental variables. It provides a reference basis for policy on breeding, environmental safety, and maintaining food safety in the production of P. monodon.

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Section: Discussionmentioning

confidence: 82%

Metagenomic Analysis of Bacterial Communities and Antibiotic Resistance Genes in Penaeus monodon Biofloc-Based Aquaculture Environments

Chen

Zhao

et al. 2022

Front. Mar. Sci.

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“…ARGs are contaminants of emerging concern, and they are not commonly monitored in the environment. However, they have strong potential in leading to negative ecological and human health effects [16,36]. Unfortunately, there are no legal regulations that define permitted levels of antibiotics or ARB and ARGs allowed to be released into the environment [2,16,17].…”

Section: Antimicrobial Resistance As a Global Health Crisismentioning

confidence: 99%

Application of TiO2-Based Photocatalysts to Antibiotics Degradation: Cases of Sulfamethoxazole, Trimethoprim and Ciprofloxacin

2021

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The extensive application of antibiotics in human and veterinary medicine has led to their widespread occurrence in a natural aquatic environment. Global health crisis is associated with the fast development of antimicrobial resistance, as more and more infectious diseases cannot be treated more than once. Sulfamethoxazole, trimethoprim and ciprofloxacin are the most commonly detected antibiotics in water systems worldwide. The persistent and toxic nature of these antibiotics makes their elimination by conventional treatment methods at wastewater treatment plants almost impossible. The application of advanced oxidation processes and heterogeneous photocatalysis over TiO2-based materials is a promising solution. This highly efficient technology has the potential to be sustainable, cost-efficient and energy-efficient. A comprehensive review on the application of various TiO2-based photocatalysts for the degradation of sulfamethoxazole, trimethoprim and ciprofloxacin is focused on highlighting their photocatalytic performance under various reaction conditions (different amounts of pollutant and photocatalyst, pH, light source, reaction media, presence of inorganic ions, natural organic matter, oxidants). Mineralization efficiency and ecotoxicity of final products have been also considered. Further research needs have been presented based on the literature findings. Among them, design and development of highly efficient under sunlight, stable, recyclable and cost-effective TiO2-based materials; usage of real wastewaters for photocatalytic tests; and compulsory assessment of products ecotoxicity are the most important research tasks in order to meet requirements for industrial application.

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“…As antibiotic resistance becomes more prevalent, the environmental factors contributing to the spread of antibiotic resistance genes are intensively investigated beyond the clinical realm (Li et al, 2020). The antibiotic resistance genes of spider gut microbiota were assessed based on a diamond search against the Resfams profile HMM database (Gibson et al, 2015).…”

Section: Analysis Of Genes Related To Carbohydrate and Antibiotic Resistancementioning

confidence: 99%

Diversity and Function of Wolf Spider Gut Microbiota Revealed by Shotgun Metagenomics

Wang

Xie

et al. 2021

Front. Microbiol.

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Wolf spiders (Lycosidae) are crucial component of integrated pest management programs and the characteristics of their gut microbiota are known to play important roles in improving fitness and survival of the host. However, there are only few studies of the gut microbiota among closely related species of wolf spider. Whether wolf spiders gut microbiota vary with habitats remains unknown. Here, we used shotgun metagenomic sequencing to compare the gut microbiota of two wolf spider species, Pardosa agraria and P. laura from farmland and woodland ecosystems, respectively. The results show that the gut microbiota of Pardosa spiders is similar in richness and abundance. Approximately 27.3% of the gut microbiota of P. agraria comprises Proteobacteria, and approximately 34.5% of the gut microbiota of P. laura comprises Firmicutes. We assembled microbial genomes and found that the gut microbiota of P. laura are enriched in genes for carbohydrate metabolism. In contrast, those of P. agraria showed a higher proportion of genes encoding acetyltransferase, an enzyme involved in resistance to antibiotics. We reconstructed three high-quality and species-level microbial genomes: Vulcaniibacterium thermophilum, Anoxybacillus flavithermus and an unknown bacterium belonging to the family Simkaniaceae. Our results contribute to an understanding of the diversity and function of gut microbiota in closely related spiders.

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Source tracking of antibiotic resistance genes in the environment — Challenges, progress, and prospects

Cited by 88 publications

References 119 publications

Metagenomic Analysis of Bacterial Communities and Antibiotic Resistance Genes in Penaeus monodon Biofloc-Based Aquaculture Environments

Metagenomic Analysis of Bacterial Communities and Antibiotic Resistance Genes in Penaeus monodon Biofloc-Based Aquaculture Environments

Application of TiO2-Based Photocatalysts to Antibiotics Degradation: Cases of Sulfamethoxazole, Trimethoprim and Ciprofloxacin

Diversity and Function of Wolf Spider Gut Microbiota Revealed by Shotgun Metagenomics

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