Source of Methyl Groups in Brain and Nerve Tissue in the Rat

Long, Mary Louisa; Weir, Donald G.; Scott, John M.

doi:10.1111/j.1471-4159.1989.tb09132.x

Cited by 7 publications

(3 citation statements)

References 13 publications

(9 reference statements)

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“…Possible suggestions for the further development of therapeutic potentials have been based on the understanding of these processes. The usual maintenance of folate concentration also depends on the activity of enzymes catalysing conjugation, methylation, oxidation and other reactions involved in folate-related metabolic pathways (3)(4)(5)(9)(10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

Folate Supplementation in Rats: Does it Cause Behavioural and Electrophysiological Changes?

et al. 2002

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Folate supplementation is becoming increasingly popular not only to decrease homocystein, a long with the finding that moderate hyperhomocysteinaemia is a cardiovascular risk factor but also diminish some other risks related to folate depletion. The aim of the study was to investigate whether folic acid supplementation leads to behavioural and electrophysiological changes in rats. Therefore, 30 male Wistar rats were divided to three groups. Each of animal groups was consisted of 10 animals. Folic acid was applied to rats by gavage for 8 weeks. Group I as a control group was taking folic acid-free water by gavage throughout the duration of the experiment. Groups II and III were treated with 2.0 mg/kg/day and 20.0 mg/kg/day of folic acid, respectively. Behavioural effects were evaluated by open field motor activity and by acoustic startle response. Electrophysiological examination was done by recording spontaneous activity and sensory evoked potentials from the visual, somatosensory, as weil as auditory cortex. No significant effects on motor activity or acoustic startle response were observed. Both doses of folic acid shortened the latency of the visual cortical evoked potential (p<0.05) and caused a slightly but nonsignificant change in the relative refractory period of a peripheral nerve. There was no effect on the electrocorticograms or on the other evoked potentials. Among the investigated organs, significant differences were observed in relative weight of kidneys and adrenal glands among the folic acid treatment at both the doses and the control groups (p<0.05).

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Section: Introductionmentioning

confidence: 99%

Folate Supplementation in Rats: Does it Cause Behavioural and Electrophysiological Changes?

et al. 2002

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“…Issues of blood–brain barrier permeability create nuances for the brain's utilization of this one‐carbon cycle (Long et al . ). Failure to maintain proper methylation patterns also seems to be a predictable and efficacious consequence of aging (Horvath ; Keleshian et al .…”

Section: Gene Transcriptionmentioning

confidence: 97%

“…This is one of the risks associated with alcohol abuse, which often gives rise to B12 deficiencies (Kruman and Fowler 2014). Issues of blood-brain barrier permeability create nuances for the brain's utilization of this one-carbon cycle (Long et al 1989). Failure to maintain proper methylation patterns also seems to be a predictable and efficacious consequence of aging (Horvath 2013;Keleshian et al 2013), whether through stochastic loss of fidelity in replication or through some other impact of the aging process on the relevant enzymes.…”

Section: Dna Methylationmentioning

confidence: 99%

Gene regulation and genetics in neurochemistry, past to future

Barger

2016

Journal of Neurochemistry

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Ask any neuroscientist to name the most profound discoveries in the field in the past 60 years, and at or near the top of the list will be a phenomenon or technique related to genes and their expression. Indeed, our understanding of genetics and gene regulation has ushered in whole new systems of knowledge and new empirical approaches, many of which could not have even been imagined prior to the molecular biology boon of recent decades. Neurochemistry, in the classic sense, intersects with these concepts in the manifestation of neuropeptides, obviously dependent upon the central dogma (the established rules by which DNA sequence is eventually converted into protein primary structure) not only for their conformation but also for their levels and locales of expression. But, expanding these considerations to non-peptide neurotransmitters illustrates how gene regulatory events impact neurochemistry in a much broader sense, extending beyond the neurochemicals that translate electrical signals

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Accumulation of galactosylsphingosine (psychosine) does not interfere with phosphorylation and methylation of myelin basic protein in the twitcher mouse

et al. 1990

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In attempts to elucidate mechanisms of demyelination in the twitcher mouse (Twi), phosphorylation and methylation of myelin basic protein (MBP) were examined in the brainstem and spinal cord of this species. Phosphorylation of MBP in isolated myelin by an endogenous kinase and an exogenous [32P]ATP was not impaired and protein kinase C activity in the brain cytosol was not reduced. When the methylation of an arginine residue of MBP was examined in slices of the brainstem and spinal cord, using [3H]methionine as a donor of the methyl groups, no difference was found between Twi and the controls. Radioactivity of the [3H] methionine residue of MBP of Twi was also similar to that of the controls. Thus, accumulation of psychosine in Twi does not interfere with the activity of endogenous kinase, methylation of MBP, and the synthesis and transport of MBP into myelin membrane.

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Source of Methyl Groups in Brain and Nerve Tissue in the Rat

Cited by 7 publications

References 13 publications

Folate Supplementation in Rats: Does it Cause Behavioural and Electrophysiological Changes?

Folate Supplementation in Rats: Does it Cause Behavioural and Electrophysiological Changes?

Gene regulation and genetics in neurochemistry, past to future

Accumulation of galactosylsphingosine (psychosine) does not interfere with phosphorylation and methylation of myelin basic protein in the twitcher mouse

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