Source of Increased Serum Beta-Hexosaminidase in Rat Liver
Cirrhosis

Hultberg, Björn; Hägerstrand, Inga; Isaksson, Anders; Joelsson, B.; Melén, K; Elsafi, Mohamed Elhassan

doi:10.1159/000469136

Cited by 14 publications

(21 citation statements)

References 13 publications

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“…Similarly, in this study äs in our earlier reports (3,6), the autophagocytotic process in degenerating cells is combined with an inerease of ß-hexosaminidase-positive granules, probably lysosomes. Confer & Stenger (20) and Kanashima et al (21) also found that degenerating hepatocytes initially respond by increasing the nümber of their lysosomes.…”

Section: Discussionsupporting

confidence: 90%

“…From earlier o f high molecular mass. Via specific (mannose-6-phosstudies in humans and rats we küow that the lysosomal phate) receptors, most of these precursors are transenzyme, ß-hexosaminidase, 1 ) is increased in serum äs ported to the lysosomes, where they are subsequently well äs in liver and spieen tissue in cholestasis and cir-dephosphorylated and proteolytically processed to genrhosis (2)(3)(4)(5). In long Standing experimental cholestasis, erate lysosomally localized mature forms (7,8).…”

Section: Introductionmentioning

confidence: 94%

“…The biochemical determination of ß-hexosaminidase in serum and liver tissue has been described (3,14). The fraction of the isoenzyme ß-hexosaminidase B was determined using enzyme immunoassay methods äs earlier described (14).…”

Section: Biochemical Analysismentioning

confidence: 99%

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Lysosomes and Human Liver Disease: A Biochemical and Immunohistochemical Study of -Hexosaminidase

Elsafi

Hultberg²,

Isaksson³

et al. 1994

CCLM

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Summary:Liver biopsies from 88 patients with different liver diseases were studied for ß-hexosaminidase activity. Liver specimens with normal light microscopical morphology showed no immunohistochemical reactivity for ß-hexosaminidase. Increased reactions were noted, mainly in hepatocytes, in biopsies from the patients with different liver diseases. A very large interindividual Variation of biochemical liver ß-hexosamindase activity occurred even within the same diagnostic group, and no group of patients showed any significant increase of liver ß-hexosaminidase activity compared with the patients with normal liver histology. Livers with positive immunohistochemistry showed increased biochemical values for ß-hexosaminidase. In patients with cholestasis due to alcohol abuse, the immunohistochemical reaetion was intense and the biochemical ß-hexosaminidase activity was significantly increased compared with non-alcöholic cholestatic cases. Furthermore, blood samples were taken from 50 patients at the same time äs the liver biopsies. These patients showed significantly increased serum ß-hexosaminidase activity compared with normal coiitrols, but no correlation was found between ß-hexosaminidase activities in the liver and serum of these patients.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 94%

See 1 more Smart Citation

Lysosomes and Human Liver Disease: A Biochemical and Immunohistochemical Study of -Hexosaminidase

Elsafi

Hultberg²,

Isaksson³

et al. 1994

CCLM

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“…In fact, Melén et al [5] found that, in intragastric CCl4-induced rat liver cirrhosis [16], the increase in serum Hex levels was greater in animals with more deteriorated liver function tests. Hultberg et al [8] re cently showed that most of the increased serum Hex activity in rat liver cirrhosis is derived from parenchymal liver cells, mainly from necrotic or atrophic hepatocytes. In man, Isaksson et al [4] found serum Hex activity much more increased in patients with alcoholic liver cirrhosis than in chronic alcoholics, who probably only had liver stea tosis.…”

Section: Discussionmentioning

confidence: 99%

“…Determination of Hex activity in the sera and liver tissue was performed according to Hultberg et al [8] as follows. 50 pi 7.5 mmol/1 nitrophenyl-2-acetamidodeoxy-B-D-glucopyranoside (from Sigma Chemical Co., St. Louis, Mo., USA), and 50 pi 1 mol/1 citrate buffer (pH 4.5) and 100 pi plasma (or liver homogenate) were incubated at 37 °C for 30 min.…”

Section: Biochemical Determinationsmentioning

confidence: 99%

β-Hexosaminidase Activity in Alcoholic Fatty Liver and in CCL-Induced Liver Fibrosis of the Rat

Antoniello¹,

Auletta²,

Vatiero³

et al. 1989

Enzyme

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β-Hexosaminidase (Hex) activity was previously found to be increased in the sera of patients with liver cirrhosis, cholestasis and acute alcohol intoxication, as well as in rats with CCL(4)-induced liver cirrhosis. We studied this enzymatic activity in the sera and liver tissue of rats with alcoholic fatty liver due to prolonged alcohol intake and CCl(4-) induced liver fibrosis in association with moderate alterations in liver function tests. Serum and liver Hex activity did not show any significant change in both experimental models. These data suggest that Hex is not an alcohol-induced enzyme, and that severe, but not moderate, liver damage can determine the increase in this lysosomal enzymatic activity.

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Hepatic accumulation of lysosomes and defective transcytotic vesicular pathways in cirrhotic rat liver

1992

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To investigate the potential role of lysosomes in cirrhosis, we analyzed the activity of lysosomal enzymes in rats exposed long-term to phenobarbital and carbon tetrachloride. The activity of lysosomal enzymes was markedly increased in the homogenate of cirrhotic livers (e.g., arylsulfatase 9 +/- S.D.2 vs. 16 +/- 6 nmoles.min-1.mg-1 in control rats and cirrhotic rats, respectively; p less than 0.001). The corresponding plasma levels were also increased (7 +/- 1 vs. 12 +/- 3 nmoles.min-1.mg-1; p less than 0.01), whereas biliary excretion was diminished (16 +/- 7 vs. 7 +/- 2 pmol.min-1.gm liver-1; p less than 0.05) in cirrhotic rats. Stereological quantification of lysosomes visualized cytochemically revealed an increase of pericanalicular lysosomes averaging 1.5 +/- 0.4 around a canaliculus in controls and 3.7 +/- 1.0 in cirrhotic rats (p less than 0.01). Because this suggested a defect in the transcellular vesicular pathway, we investigated the biliary excretion of horseradish peroxidase and epidermal growth factor in perfused livers. Bile flow and total horseradish peroxidase excretion were similar in control rats and cirrhotic rats. However, the early peak of biliary horseradish peroxidase excretion--usually taken as evidence of paracellular transport--was increased in cirrhotic rats (13 +/- 7 vs. 57 +/- 22%; p less than 0.01), whereas the second peak--reflecting the transcellular vesicular pathway(s)--was markedly reduced (87 +/- 7 vs. 43 +/- 22%; p less than 0.001). A similar reduction in the biliary excretion of intact epidermal growth factor and of its degradation products was found. These results demonstrate an increased number of lysosomes in hepatocytes of cirrhotic livers; this appears to be the result of accumulation rather than proliferation, in view of the reduced transcellular vesicular movement of different markers into bile.

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Source of Increased Serum Beta-Hexosaminidase in Rat Liver Cirrhosis

Cited by 14 publications

References 13 publications

Lysosomes and Human Liver Disease: A Biochemical and Immunohistochemical Study of -Hexosaminidase

Lysosomes and Human Liver Disease: A Biochemical and Immunohistochemical Study of -Hexosaminidase

β-Hexosaminidase Activity in Alcoholic Fatty Liver and in CCL-Induced Liver Fibrosis of the Rat

Hepatic accumulation of lysosomes and defective transcytotic vesicular pathways in cirrhotic rat liver

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