Source and Portal of Entry of Bacteria Found in Bruised Poultry Tissue1

Abstract

HAMDY, M. K. (University of Georgia, Athens), N. D. BAR-TON, AND W. E. BROWN. Source and portal of entry of bacteria found in bruised poultry tissue. Appl. Microbiol. 12:464-469. 1964.-Bacteriological studies revealed that normal tissue, air sacs, feathers, skin of birds, poultry feed, gut, and chicken droppings were sources of the predominant organisms, including staphylococci, found in bruised poultry tissue. Further investigation of normal tissue revealed that after the intramuscular injection of Staphylococcus aureus, marker strain (MS), the organism was eliminated from these tissues within 7 days. However, when these tissues were traumatized 3 days after injection, the number of the test organism increased, and the organism was present on the 7th day after inoculation. Poultry feed and fecal material contained a large number of staphylococci identical to those isolated from bruised tissue (McCarthy, Brown, and Hamdy, 1963), thereby implicating the gut as a possible portal of entry. When a pathogenic marker strain of S. aureus was established in the intestinal tract of chickens by administering an active culture of this organism either in their drinking water or by gavage, it was recovered from the traumatized tissue. The incidence of positive culture of S. aureus MS in these tissues correlated with age of bruise, reaching 22 to 33% immediately after contusion and at the early stages of healing (1 to 3 days post bruise) and decreasing thereafter from 11 to 0% on the 4th through 6th days after bruise infliction. The air sac was also found to be a site by which bacteria may enter the traumatized tissues, but to a limited extent.

Fate of Staphylococcus aureus in Bruised Tissue12

“…McCarthy et al (1963) also isolated S. aureus identical to those found in bruised tissue from poultry feed and fecal material of chickens. Hamdy, Barton, and Brown (1964) found that both the gut and air sacs were possible portals of entry of microorganisms to the traumatized areas. These authors also showed that when a virulent marker strain of S. aureus was established in the gut of the chickens by administering an active culture of this organism either in their drinking water or by gavage, it was recovered from the bruised tissues.…”