Sound induced expression of c-Fos in GABA positive neurones of the gerbil cochlear nucleus

Gleich, Otto; Bielenberg, Katrin; Strutz, Jürgen

doi:10.1097/00001756-199512290-00006

Cited by 6 publications

(4 citation statements)

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“…Adams (1995) reported that sound-induced Fos expression in the core of the VCN was largely from the smaller stellate neurons and not from the larger bushy neurons. At least some of these may have represented inhibitory neurons (Gleich et al, 1995). In the present study, we found abundant c-fos expression throughout the magnocellular areas of the VCN including those areas that are populated almost exclusively by spherical bushy cells, globular bushy cells, stellate cells, or octopus cells (e.g., Harrison and Feldman, 1970).…”

Section: Comparison With Sound-induced Expression Of the Fos Proteinsupporting

confidence: 61%

Effects of stimulus frequency and intensity on c-fos mRNA expression in the adult rat auditory brainstem

Marie¹,

Luo²,

Ryan³

1999

J. Comp. Neurol.

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Induction of the cellular fos gene (c‐fos) is one of the earliest transcriptional changes observed following neuronal excitation. Although not an activity marker in the strict electrophysiological sense, many neurons in the central nervous system increase their c‐fos expression after periods of sustained stimulation at physiological levels of intensity. In the present study, induction of c‐fos mRNA expression was examined in the auditory brainstem after 1 hour of continuous free‐field acoustic stimulation. Sprague‐Dawley rats were exposed to pure tones of 2, 8, 16, or 32 kHz or half‐octave noise bands centered on 2, 8, or 32 kHz at 80–120 dB SPL. Stimulation‐induced c‐fos mRNA expression was evident at all levels of the auditory brainstem, and this expression was intensity dependent. In some brain areas, induced expression manifested a clear tonotopic organization, i.e., in dorsal, posteroventral, and anteroventral cochlear nuclei, and in the medial nucleus of the trapezoid body. The inferior colliculus exhibited multiple tonotopic representations. The dorsal nucleus of the lateral lemniscus had a crude tonotopy. Although expression was present, tonotopy was not evident in periolivary nuclei or in the ventral or intermediate nuclei of the lateral lemniscus. Free‐field diotic stimulation did not induce c‐fos mRNA expression in the medial or lateral superior olivary nuclei. Expression was induced in the lateral superior olive by dichotic stimulation (after a unilateral cochlear ablation), and that expression was tonotopically organized. The results suggest that stimulation‐induced c‐fos mRNA expression can be an effective way of mapping neuronal activity in the central auditory system under both normal and pathological conditions. J. Comp. Neurol. 404:258–270, 1999. © 1999 Wiley‐Liss, Inc.

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Section: Comparison With Sound-induced Expression Of the Fos Proteinsupporting

confidence: 61%

Effects of stimulus frequency and intensity on c-fos mRNA expression in the adult rat auditory brainstem

Marie¹,

Luo²,

Ryan³

1999

J. Comp. Neurol.

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“…We have previously observed a similar secondary peak of delayed c-fos expression in the ventral cochlear nucleus 24 hours after intense noise exposure (our unpublished data). These observations may reflect long-term changes in neural processing pathways, such as changes in inhibitory interneurons [83,84]. The RC is involved in spatial learning and navigation.…”

Section: Discussionmentioning

confidence: 99%

Effects of Antioxidant Treatment on Blast-Induced Brain Injury

Ewert

Cheng

et al. 2013

PLoS ONE

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Blast-induced traumatic brain injury has dramatically increased in combat troops in today’s military operations. We previously reported that antioxidant treatment can provide protection to the peripheral auditory end organ, the cochlea. In the present study, we examined biomarker expression in the brains of rats at different time points (3 hours to 21 days) after three successive 14 psi blast overpressure exposures to evaluate antioxidant treatment effects on blast-induced brain injury. Rats in the treatment groups received a combination of antioxidants (2,4-disulfonyl α-phenyl tertiary butyl nitrone and N-acetylcysteine) one hour after blast exposure and then twice a day for the following two days. The biomarkers examined included an oxidative stress marker (4-hydroxy-2-nonenal, 4-HNE), an immediate early gene (c-fos), a neural injury marker (glial fibrillary acidic protein, GFAP) and two axonal injury markers [amyloid beta (A4) precursor protein, APP, and 68 kDa neurofilament, NF-68]. The results demonstrate that blast exposure induced or up-regulated the following: 4-HNE production in the dorsal hippocampus commissure and the forceps major corpus callosum near the lateral ventricle; c-fos and GFAP expression in most regions of the brain, including the retrosplenial cortex, the hippocampus, the cochlear nucleus, and the inferior colliculus; and NF-68 and APP expression in the hippocampus, the auditory cortex, and the medial geniculate nucleus (MGN). Antioxidant treatment reduced the following: 4-HNE in the hippocampus and the forceps major corpus callosum, c-fos expression in the retrosplenial cortex, GFAP expression in the dorsal cochlear nucleus (DCN), and APP and NF-68 expression in the hippocampus, auditory cortex, and MGN. This preliminary study indicates that antioxidant treatment may provide therapeutic protection to the central auditory pathway (the DCN and MGN) and the non-auditory central nervous system (hippocampus and retrosplenial cortex), suggesting that these compounds have the potential to simultaneously treat blast-induced injuries in the brain and auditory system.

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“…For the induction of c-fos, all gerbils (except for I control and 1 animal deprived at P84) w'ere exposed for 90 min under free-ficld conditions in a sound-attenuating chamber to loud (94 dB SPL) pulsed (100 ms on, 5/s) broad-band noise [33], Imme diately after the sound exposure, the animals received a lethal dose of anesthetic. A transcardial perfusion w'ith 0.9% NaCl over 1-2 min for the removal of blood was followed for 30 min by 200-300 ml of a fresh solu tion of 4% paraformaldehyde in 0 .1 M phosphate buff er pH 7.4.…”

Section: Methodsmentioning

confidence: 99%

“…The significance of c-fos expression in the CN is not well understood, although there is evidence that inhibitory GABAergic neurons express c-fos after acoustic stimulation [33]. The expression of GABA continues to mature in the gerbil CN during the weeks after the onset of hearing, and appears mature at the light microscopical level by the age of 4 weeks [69 and Gleich and Vater, in preparation].…”

Section: Effects O F a Conductive Hearing Loss On C-fos Expressionmentioning

confidence: 99%

Age-Dependent Effects of the Onset of a Conductive Hearing Loss on the Volume of the Cochlear Nucleus Subdivisions and the Expression of c- fos in the Mongolian Gerbil (Meriones unguiculatus)

Gleich

Strutz²

1997

Audiol Neurotol

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A monaural conductive hearing loss was induced by interrupting the chain of the middle ear ossicles on the right side in gerbils of four different age groups (Pl 2–14, P20–21, P42 and P84). The volumes of the cochlear nucleus subdivisions and the number of cells that expressed immunoreactivity for c-fos after noise stimulation were determined on the left and right side in the deprived animals, and in undeprived control animals when they reached the age of 6 months. The anteroventral cochlear nucleus on the deprived side was reduced in volume when the deprivation started before the age of 3 months. The other cochlear nucleus subdivisions showed no systematic age-dependent reductions. The expression of c-fos in the dorsal cochlear nucleus appeared more resistant to a hearing loss, with deprivation being more effective in younger animals. c-fos expression was also dramatically reduced in the ventral cochlear nucleus, regardless of age at the onset of hearing loss.

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Sound induced expression of c-Fos in GABA positive neurones of the gerbil cochlear nucleus

Cited by 6 publications

References 0 publications

Effects of stimulus frequency and intensity on c-fos mRNA expression in the adult rat auditory brainstem

Effects of stimulus frequency and intensity on c-fos mRNA expression in the adult rat auditory brainstem

Effects of Antioxidant Treatment on Blast-Induced Brain Injury

Age-Dependent Effects of the Onset of a Conductive Hearing Loss on the Volume of the Cochlear Nucleus Subdivisions and the Expression of c- fos in the Mongolian Gerbil (Meriones unguiculatus)

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