Sorting of sphingolipids in the endocytic pathway of HT29 cells.

Kok, Jan; Babià, T; Hoekstra, Dick

doi:10.1083/jcb.114.2.231

Cited by 81 publications

(50 citation statements)

References 14 publications

(19 reference statements)

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“…Similar behavior is observed for short chain lipid analogs such as C 6 -NBD-SM, which recycle efficiently upon internalization (33,34), whereas some lipid analogs containing long, saturated acyl chains (e.g. Rh-PE and some glycosphingolipids) (42,43) are targeted to late endocytic compartments.…”

Section: Altered Diic 16 Trafficking In Cholesterol-depleted or Cho-smentioning

confidence: 58%

Effects of Cholesterol Depletion and Increased Lipid Unsaturation on the Properties of Endocytic Membranes

Hao

Mukherjee

Sun

et al. 2004

Journal of Biological Chemistry

100

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Lipid analogs with dialkylindocarbocyanine (DiI) head groups and short or unsaturated hydrocarbon chains (e.g. DiIC 12 and FAST DiI) enter the endocytic recycling compartment efficiently, whereas lipid analogs with long, saturated tails (e.g. DiIC 16 and DiIC 18 ) are sorted out of this pathway and targeted to the late endosomes/lysosomes (Mukherjee, S., Soe, T. T., and Maxfield, F. R. (1999) J. Cell Biol. 144, 1271-1284). This differential trafficking of lipid analogs with the same polar head group was interpreted to result from differential partitioning to different types of domains with varying membrane order and/or curvature. Here we investigate the system further by monitoring the trafficking behavior of these lipid analogs under conditions that alter domain properties. There was a marked effect of cholesterol depletion on the cell-surface distribution and degree of internalization of the lipid probes. Furthermore, instead of going to the late endosomes/lysosomes as in control cells, long chain DiI analogs, such as DiIC 16 , were sorted to the recycling pathway in cholesterol-depleted cells. We confirmed that this difference was due to a change in overall membrane properties, and not cholesterol levels per se, by utilizing a Chinese hamster ovary cell line that overexpressed transfected stearoyl-CoA desaturase 1, a rate-limiting enzyme in the production of monounsaturated fatty acids. These cells have a decrease in membrane order because they contain a much larger fraction of unsaturated fatty acids. These cells showed alteration of DiI trafficking very similar to cholesterol-depleted cells. By using cold Triton X-100 extractability of different lipids as a criterion to determine the membrane properties of intracellular organelles, we found that the endocytic recycling compartment has abundant detergent-resistant membranes, in contrast to the late endosomes and lysosomes.Lipids and proteins associated with the cell surface vary in their lateral and transbilayer distribution, as well as the rate at which they are internalized from the plasma membrane. Once inside the cell, they can potentially be delivered to a large variety of organelles by selective partitioning in a series of sorting steps associated with vesicle or tubule formation (1). Although many specific peptide motifs and protein-protein interactions that determine the distribution and trafficking of transmembrane proteins have been characterized (1, 2), the principles underlying lipid sorting and trafficking remain relatively unclear. Although the intracellular destinations and sorting decisions for a variety of lipids and lipid analogs have been investigated in recent years (1, 3), a coherent general set of sorting rules for lipids has yet to emerge.Part of the difficulty in understanding lipid sorting and distribution in the cell arises from the fact that this is the result of a complex interplay between the specific chemistries of individual lipid molecules (e.g. their size, hydrophobicity, head group to acyl chain cross-sectional ratio, charge...

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Section: Altered Diic 16 Trafficking In Cholesterol-depleted or Cho-smentioning

confidence: 58%

Effects of Cholesterol Depletion and Increased Lipid Unsaturation on the Properties of Endocytic Membranes

Hao

Mukherjee

Sun

et al. 2004

Journal of Biological Chemistry

100

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“…While the Golgi system is thought to be involved in the activation of ricin, several authors suggested that, at least with some cellular strains, ricin may accumulate in endosomal structures from which the A chain or the intact toxin could escape directly into the cytoplasm [20,21]; some results indicate that activation is also possible in the cytoplasm, via disulfide bond reduction [ 1 l] or proteolytic processing [22]. We searched, but did not find evidence for a differential processing of the two subunits of ricin during translocation from the plasma membrane to the Golgi (see Fig.…”

Section: Discussionmentioning

confidence: 99%

Intracellular dynamics of ricin followed by fluorescence microscopy on living cells reveals a rapid accumulation of the dimeric toxin in the Golgi apparatus

et al. 1994

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The intracellular dynamics of fluorescent conjugates of the toxic lectin ricin was followed by video fluorescence microscopy on living CHO cells, demonstrating that the ricin heterodimer and its isolated B chain, after binding to the plasma membrane receptors, migrate to and accumulate in the Golgi apparatus following internalization. A ricin derivative labelled with fluorescein on the A chain and rhodamine on the B chain did not display significant splitting of the A-B heterodimer during translocation of the toxin to the Golgi; this novel finding provides support for the hypothesis that further processing of ricin takes place in this cellular compartment.

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“…Moreover, not all lipids follow the same processing path. Since Pagano's pioneering studies began, other researchers have used the same basic approach to study the sorting of lipids within differentiated or non-differentiated adenocarcinoma cells [34] or within polarized epithelial cells when the fluorescent lipids are introduced by spontaneous transfer to either the basolateral or apical cell surfaces [35][36][37].…”

Section: Ii-a Value As An Experimental Toolmentioning

confidence: 99%

Spontaneous lipid transfer between organized lipid assemblies

Brown

1992

Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes

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Sorting of sphingolipids in the endocytic pathway of HT29 cells.

Cited by 81 publications

References 14 publications

Effects of Cholesterol Depletion and Increased Lipid Unsaturation on the Properties of Endocytic Membranes

Effects of Cholesterol Depletion and Increased Lipid Unsaturation on the Properties of Endocytic Membranes

Intracellular dynamics of ricin followed by fluorescence microscopy on living cells reveals a rapid accumulation of the dimeric toxin in the Golgi apparatus

Spontaneous lipid transfer between organized lipid assemblies

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