Sorting Nexin 17 Interacts Directly with Kinesin Superfamily KIF1Bβ Protein

Abstract

KIF1Bβ is a member of the Kinesin superfamily proteins (KIFs), which are microtubule-dependent molecular motors that are involved in various intracellular organellar transport processes. KIF1Bβ is not restricted to neuronal systems, however, is widely expressed in other tissues, even though the function of KIF1Bβ is still unclear. To elucidate the KIF1Bβ-binding proteins in non-neuronal cells, we used the yeast two-hybrid system, and found a specific interaction of KIF1Bβ and the sorting nexin (SNX) 17. The C-terminal region of SNX17 is required for the binding with KIF1Bβ. SNX17 protein bound to the specific region of KIF1Bβ (813-916. aa), but not to other kinesin family members. In addition, this specific interaction was also observed in the Glutathione S-transferase pull-down assay. An antibody to SNX17 specifically co-immunoprecipitated KIF1Bβ associated with SNX17 from mouse brain extracts. These results suggest that SNX17 might be involved in the KIF1Bβ-mediated transport as a KIF1Bβ adaptor protein.

Myosin VI contributes to malignant proliferation of human glioma cells

“…Myosins are actin-based motor proteins, coupling ATP hydrolysis to mechanical motion along actin filaments. Many myosin family have been implicated as molecular motors, which play important roles in the transport of intracellular organelles [ 7 ]. They are known to contribute to essential cellular functions, including secretion, cell division, differentiation, and migration [ 8 ].…”

Human Papillomavirus L2 Facilitates Viral Escape from Late Endosomes via Sorting Nexin 17

“…SNX17 has a PX domain followed by a protein 4.1, ezrin, radixin and moesin (FERM)‐like domain ; it is localized to early endosomes and recycling tubules and is primarily involved in endosomal recycling. SNX 17 interacts with the members of low‐density lipoprotein receptor family , P‐selectin , amyloid precursor protein , cytosolic factors Krit1 and Kif1B , and the scavenger receptor FEEL‐1/stabilin‐1 , modulating their internalization or their recycling from endosomes to the cell surface or both.…”

Structural Determinants for Binding of Sorting Nexin 17 (SNX17) to the Cytoplasmic Adaptor Protein Krev Interaction Trapped 1 (KRIT1)

“…Although the binding partners of SNX17 are mostly transmembrane proteins, SNX17 has also been shown to bind several cytoplasmic proteins including Ras GTPases (12), the kinesin-like protein Kif1B (20), and SH3 domain-containing proteins including PLC␥ (21). Another reported cytoplasmic binding partner for SNX17 is Krev interaction trapped 1 (KRIT1) (22).…”