Sorting biomolecules with microdevices

Chou, Chia-Fu; Austin, Robert H.; Bakajin, Olgica; Tegenfeldt, Jonas O.; Castelino, Judith; Chan, Shirley S.; Cox, Edward C.; Craighead, Harold G.; Darnton, N.; Duke, Thomas; Han, Jongyoon; Turner, Steve

doi:10.1002/(sici)1522-2683(20000101)21:1<81::aid-elps81>3.0.co;2-#

Cited by 71 publications

(61 citation statements)

References 24 publications

(18 reference statements)

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“…In 1980, Cannell and Rondelez 45 considered the diffusion of monodisperse polystyrene chains ͑molecular weights up to 600 000͒ through porous membranes. For 0.1Ͻr s /r p Ͻ0.5, they found their data followed the Renkin 2 , with Faxen's expression for the diffusion of a sphere along the centerline of a channel. The dotted curve in Fig.…”

Section: ͑29͒mentioning

confidence: 87%

“…[1][2][3][4][5][6][7] For instance, in certain implementations of exonucleolytic sequence analysis it is desirable to link an individual long DNA strand to a surface ͑e.g., a bead͒ without manual intervention. 6,7 The ability to engineer such tasks will be greatly improved by predictive computational tools for polymer chains in microfluidic geometries.…”

Section: Introductionmentioning

confidence: 99%

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Effect of confinement on DNA dynamics in microfluidic devices

Jendrejack

Schwartz

Graham

et al. 2003

The Journal of Chemical Physics

166

193

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The dynamics of dissolved long-chain macromolecules are different in highly confined environments than in bulk solution. A computational method is presented here for detailed prediction of these dynamics, and applied to the behavior of ϳ1-100 m DNA in micron-scale channels. The method is comprised of a self-consistent coarse-grained Langevin description of the polymer dynamics and a numerical solution of the flow generated by the motion of polymer segments. Diffusivity and longest relaxation time show a broad crossover from free-solution to confined behavior centered about the point HϷ10S b , where H is the channel width and S b is the free-solution chain radius of gyration. In large channels, the diffusivity is similar to that of a sphere diffusing along the centerline of a pore. For highly confined chains (H/S b Ӷ1), Rouse-type molecular weight scaling is observed for both translational diffusivity and longest relaxation time. In the highly confined region, the scaling of equilibrium length and relaxation time with H/S b are in good agreement with scaling theories. In agreement with the results of Harden and Doi ͓J. Phys. Chem. 96, 4046 ͑1992͔͒, we find that the diffusivity of highly confined chains does not follow the scaling relation predicted by Brochard and de Gennes ͓J. Chem. Phys. 67, 52 ͑1977͔͒; that relationship does not account for the interaction between chain and wall.

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Section: ͑29͒mentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Effect of confinement on DNA dynamics in microfluidic devices

Jendrejack

Schwartz

Graham

et al. 2003

The Journal of Chemical Physics

166

193

View full text Add to dashboard Cite

show abstract

“…One of the challenges encountered in describing the operation of real electrophoresis-based ratchets is the faithful representation of the field lines in microfabricated electrophoretic devices. A vivid example of this difficulty is given in a recent article by Austin et al [45] where they consider the curved field lines present in an asymmetric array of insulating deflectors; Austin's group [46] has successfully used this type of array to separate DNA molecules in a continuous mode. They show that if the obstacles are perfect insulators and if the particle density is symmetric, then there can be no deflection of particles on average, so no separation should be observed.…”

Section: Ratchetsmentioning

confidence: 99%

Theory of DNA electrophoresis (∼ 1999 –2002 ½)

et al. 2002

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Over the last two decades, the introduction of new methods such as pulsed-field gel electrophoresis and capillary array electrophoresis has made it possible to map and sequence entire genomes, including our own. The development of these experimental methods has been helped by the progress of theoretical and computational sciences, and the interactions between these three modi operandi of modern science are still pushing the limits of our technologies. We now see a clear trend towards proteomics and microfluidic (even nanofluidic!) devices. In this review, we take a look at the progress of the field over the last 3 years using the glasses of the theoretical scientist and focusing mostly on new ideas and concepts. About a dozen different subfields are discussed and reviewed. We conclude by giving a commented list of some of the best review articles published over the last 2-3 years.

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“…Such devices, in particular "obstacle course" microlithographic arrays [1][2][3][4][5], have received considerable attention in recent years in connection with their use as chromatographic separation media. These arrays are constructed by etching a spatially periodic sequence of raised obstacles onto a polymer mask, using microlithography techniques pioneered in the production of microelectronic devices.…”

Section: Introductionmentioning

confidence: 99%

“Vector Chromatography”: Modeling Micropatterned Separation Devices

Dorfman

Brenner

2001

Journal of Colloid and Interface Science

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A repetitive sequence of quiescent fluid layers of differing viscosities through which small spherical Brownian particles move is analyzed so as to illustrate in a simple context how the theory of macrotransport processes, a generalization of Taylor dispersion theory, may be employed to rigorously analyze spatially periodic micropatterned chromatographic separation devices for circumstances in which the solute species to be separated are animated by the action of species-specific external forces oriented asymmetrically relative to the body-fixed pattern. In the generic "vector" separation scheme, illustrated by our elementary example, the different species undergoing separation move, on average, in different directions relative to pattern-fixed axes, whence their chromatographic sorting is effected according to their different mean angular trajectories through the device. This scheme differs fundamentally from traditional "scalar" chromatographic separation schemes, wherein all species move on average parallel to the animating force (including circumstances in which they are passively entrained in a unidirectional solvent flow) and hence for which the sorting is effected by the relative speeds of the several species through the chromatographic column.Vector chromatography is quantified by two global "macrotransport coefficients," namely the solute mobility dyadic M * (representing the tensor proportionality coefficient between the mean solute velocity vector U * and the external force vector F acting upon the solute molecules) and the dispersivity dyadic D * (resulting from the deviation of the instantaneous position of the particle from its mean position based upon its mean velocity vector). In the present example these coefficients are studied parametrically as functions of: (i) the orientation of the external force relative to the symmetry axis of the fluid layers; (ii) the local viscosity distribution within a layer; (iii) the vector particle Peclet number (constructed from the vector force, the length of the viscosity period, and the Boltzmann factor kT ); and (iv) the thermodynamic interphase solute partition distribution coefficient between the two fluid layers comprising a unit cell.

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Sorting biomolecules with microdevices

Cited by 71 publications

References 24 publications

Effect of confinement on DNA dynamics in microfluidic devices

Effect of confinement on DNA dynamics in microfluidic devices

Theory of DNA electrophoresis (∼ 1999 –2002 ½)

“Vector Chromatography”: Modeling Micropatterned Separation Devices

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