Sorted B cell transcriptomes point towards actively regulated B cell responses during ongoing chronic hepatitis B infections

Hees, Stijn Van; Cuypers, Bart; Bourgeois, Stefan; Groothuismink, Zwier M. A.; Meysman, Pieter; Vlies, Pieter van der; Knegt, Rob de; Vonghia, Luisa; Michielsen, Peter; Francque, Sven; Laukens, Kris; Boonstra, André; Vanwolleghem, Thomas

doi:10.1016/j.cellimm.2021.104283

Cited by 9 publications

(15 citation statements)

References 34 publications

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“…The global B-cell transcriptome of CHB showed an activated status in the immune active and inactive carrier phase, with upregulated CD83, CD300c, CXCR4, CD69 levels and various innate stimulating genes. 76 An activating signature was also found by Salimzadeh et al in sorted MBC subsets from patients with CHB, e.g. upregulated CD83 levels.…”

Section: Phenotype and Function Of B Cells In Chbmentioning

confidence: 64%

“…We recently corroborated this by RNA sequencing on sorted intrahepatic and peripheral global CD19+ B cells. The global B cell transcriptome of CHB showed an activated status in the IA and inactive carrier (IC) phase, with upregulated CD83, CD300c, CXCR4, CD69 levels and various innate stimulating genes [76]. An activating signature was also found by Salimzadeh et al in sorted MBC subsets from CHB patients, with e.g.…”

Section: Global B Cells In Chb: Activated Vs Exhausted?mentioning

confidence: 64%

“…In addition, we found gene expression profiles of intrahepatic B cells from CHB patients to be very different from their paired peripheral counterparts. The former upregulated the BCR and several immune signaling pathways compared to peripheral blood B lymphocytes, suggesting a process of extrafollicular MBC formation [53,76]. Differently, Burton et al found intrahepatic B cells to be enriched in AtMBC [7].…”

Section: Global B Cells In Chb: Activated Vs Exhausted?mentioning

confidence: 98%

“…The former upregulated the BCR and several immune signalling pathways compared to peripheral blood B lymphocytes, suggesting a process of extrafollicular MBC formation. 53 , 76 Conversely, Burton et al found intrahepatic B cells to be enriched in AtMBCs. 7 …”

Section: Phenotype and Function Of B Cells In Chbmentioning

confidence: 99%

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Humoral immunity in hepatitis B virus infection: Rehabilitating the B in HBV

et al. 2022

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Summary Insights into the immunopathogenesis of chronic HBV infections are fundamental in the quest for novel treatment approaches aimed at a functional cure. While much is known about the ineffective HBV-specific T-cell responses that characterise persistent HBV replication, B cells have been left largely understudied. However, an important role for humoral immunity during the natural history of HBV infections, as well as after functional cure, has been inadvertently revealed by the occurrence of HBV flares following B cell-depleting treatments. Herein, we review our current understanding of the role of the humoral immune response in chronic HBV, both at the level of HBV-specific antibody production and at the phenotypic and broader functional level of B cells. The recent development of fluorescently labelled HBV proteins has given us unprecedented insights into the phenotype and function of HBsAg- and HBcAg-specific B cells. This should fuel novel research into the mechanisms behind dysfunctional HBsAg-specific and fluctuating, possibly pathogenic, HBcAg-specific B-cell responses in chronic HBV. Finally, novel immunomodulatory treatments that partly target B cells are currently in clinical development, but a detailed assessment of their impact on HBV-specific B-cell responses is lacking. We plead for a rehabilitation of B-cell studies related to both the natural history of HBV and treatment development programmes.

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Section: Phenotype and Function Of B Cells In Chbmentioning

confidence: 64%

Section: Global B Cells In Chb: Activated Vs Exhausted?mentioning

confidence: 64%

Section: Global B Cells In Chb: Activated Vs Exhausted?mentioning

confidence: 98%

Section: Phenotype and Function Of B Cells In Chbmentioning

confidence: 99%

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Humoral immunity in hepatitis B virus infection: Rehabilitating the B in HBV

et al. 2022

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“…For example, treatment with the anti-CD20 monoclonal antibody (rituximab) is known to reactivate HBV replication, which can lead to hepatic relapses even in patients with cleared infection (4). In this issue, van Hees et al investigated the extent to which B-cell activity is related to the different phase of hepatitis B (CHB) infection (5). They profiled the transcriptome of peripheral and intrahepatic B cells between the four clinical phases of CHB infection and healthy controls.…”

Section: Editorial On the Research Topicmentioning

confidence: 99%

Editorial: The B-Side of B Cells

et al. 2021

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Microfluidic systems in clinical diagnosis

Lapizco‐Encinas

Zhang

2022

Electrophoresis

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The use of microfluidic devices is highly attractive in the field of biomedical and clinical assessments, as their portability and fast response time have become crucial in providing opportune therapeutic treatments to patients. The applications of microfluidics in clinical diagnosis and point‐of‐care devices are continuously growing. The present review article discusses three main fields where miniaturized devices are successfully employed in clinical applications. The quantification of ions, sugars, and small metabolites is examined considering the analysis of bodily fluids samples and the quantification of this type of analytes employing real‐time wearable devices. The discussion covers the level of maturity that the devices have reached as well as cost‐effectiveness. The analysis of proteins with clinical relevance is presented and organized by the function of the proteins. The last section covers devices that can perform single‐cell metabolomic and proteomic assessments. Each section discusses several strategically selected recent reports on microfluidic devices successfully employed for clinical assessments, to provide the reader with a wide overview of the plethora of novel systems and microdevices developed in the last 5 years. In each section, the novel aspects and main contributions of each reviewed report are highlighted. Finally, the conclusions and future outlook section present a summary and speculate on the future direction of the field of miniaturized devices for clinical applications.

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Sorted B cell transcriptomes point towards actively regulated B cell responses during ongoing chronic hepatitis B infections

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Cited by 9 publications

References 34 publications

Humoral immunity in hepatitis B virus infection: Rehabilitating the B in HBV

Humoral immunity in hepatitis B virus infection: Rehabilitating the B in HBV

Editorial: The B-Side of B Cells

Microfluidic systems in clinical diagnosis

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