Sorption of penicillium‐cellulase on cellulose and lignin

Abstract

SnniiiiurySorption of Penicillium cellulase onto cotton linters samples differing in physical structure and onto KusoN-lignin from spruce has been investigated by determining cellobiase, CMCL-and "linters-activity" as well as protein content of a cellulase culture filtrate before and after contact with the sorbent a t different temperature for different time intervals. CMCL-and cellobiase-activity were found to be reduced much less by the sorption process onto the cellulose samples than "tinters-activity" and protein content. Sorption increased in the order: untreated linters < mercerized linters < wet fibrillated ("colloidal milled") linters. With the KLAsON-lignin also a considerable sorption of the cellulase system was observed. Results are discussed with respect to preferential sorption of enzyme components.

Effect of enzyme supplementation at moderate cellulase loadings on initial glucose and xylose release from corn stover solids pretreated by leading technologies

“…This is consistent with reports that the effectiveness of b-glucosidase drops with increasing chain lengths of soluble celloligomers (Wilson et al, 1994). Furthermore, b-glucosidase is well known to lose activity by exposure to heat and agitation (Aguado et al, 1995;Gunjikar et al, 2001;Mukataka et al, 1983), nonproductive binding with lignin (Shawky et al, 1984;Sutcliffe and Saddler, 1986;Tatsumoto et al, 1988;Xu et al, 2008;, and the effect of lignin degradation compounds (Garcia-Aparicio et al, 2006;Kaya et al, 1999;Vohra et al, 1980). In addition, the effectiveness of a given b-glucosidase loading may vary with the type of substrate, pretreatment method, substrate loading, and time of hydrolysis (Breuil et al, 1992;Gruno et al, 2004;Stockton et al, 1991;Tengborg et al, 2001).…”

Conversion of Rice Straw to Fermentable Sugars and Bioethanol by Mfex Pretreatment and Sequential Fermentation

“…New balanced enzymatic complexes containing optimal combinations to effectively modify the complex structure of lignocellulosic materials are to be developed (Shawky, Kasulke, Philipp, Schulz &Hirte 1984, andBahaa, Manal, Eman, Mohsen &Ghada 2011). The main hydrolysis product of cellulose is glucose, whereas the hemicellulose gives rise to several pentoses and hexoses (Taherzadeh & Niklasson, 2004).…”