Sorghum mutant RGdisplays antithetic leaf shoot lignin accumulation resulting in improved stem saccharification properties

Petti, Carloalberto; Harman‐Ware, Anne E.; Tateno, Mizuki; Kushwaha, Rekha; Shearer, Andrew; Downie, A. Bruce; Crocker, Mark; DeBolt, Seth

doi:10.1186/1754-6834-6-146

Cited by 24 publications

(29 citation statements)

References 72 publications

(70 reference statements)

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“…3 F), one is associated with lignin (c), while all others correlate with structural carbohydrates (a, g and j). As has been reported for sorghum ( Petti et al ., 2013 ), the prominence of band c in our data suggests higher amounts of S lignin in stem when compared with leaf tissues. However, given that the remaining three major PC1 loadings coincide with carbohydrate bands, it is likely that overall compositional shifts between leaf and stem cell-wall samples are more significant in their polysaccharide fractions.…”

Section: Discussionsupporting

confidence: 83%

Genotype, development and tissue-derived variation of cell-wall properties in the lignocellulosic energy crop Miscanthus

et al. 2014

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Background and AimsSpecies and hybrids of the genus Miscanthus contain attributes that make them front-runners among current selections of dedicated bioenergy crops. A key trait for plant biomass conversion to biofuels and biomaterials is cell-wall quality; however, knowledge of cell-wall composition and biology in Miscanthus species is limited. This study presents data on cell-wall compositional changes as a function of development and tissue type across selected genotypes, and considers implications for the development of miscanthus as a sustainable and renewable bioenergy feedstock.MethodsCell-wall biomass was analysed for 25 genotypes, considering different developmental stages and stem vs. leaf compositional variability, by Fourier transform mid-infrared spectroscopy and lignin determination. In addition, a Clostridium phytofermentans bioassay was used to assess cell-wall digestibility and conversion to ethanol.Key ResultsImportant cell-wall compositional differences between miscanthus stem and leaf samples were found to be predominantly associated with structural carbohydrates. Lignin content increased as plants matured and was higher in stem tissues. Although stem lignin concentration correlated inversely with ethanol production, no such correlation was observed for leaves. Leaf tissue contributed significantly to total above-ground biomass at all stages, although the extent of this contribution was genotype-dependent.ConclusionsIt is hypothesized that divergent carbohydrate compositions and modifications in stem and leaf tissues are major determinants for observed differences in cell-wall quality. The findings indicate that improvement of lignocellulosic feedstocks should encompass tissue-dependent variation as it affects amenability to biological conversion. For gene–trait associations relating to cell-wall quality, the data support the separate examination of leaf and stem composition, as tissue-specific traits may be masked by considering only total above-ground biomass samples, and sample variability could be mostly due to varying tissue contributions to total biomass.

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Section: Discussionsupporting

confidence: 83%

Genotype, development and tissue-derived variation of cell-wall properties in the lignocellulosic energy crop Miscanthus

et al. 2014

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“…In addition to the bmr phenotype, additional phenotypic mutants related to the composition of the SCW have been identified. Petti et al (2013Petti et al ( , 2015 identified that REDforGREEN and dwarf1.1 mutants affected lignin and cellulose abundance in leaves and stems, in addition to their respective red coloration and shortened internodes.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional Regulation of Sorghum Stem Composition: Key Players Identified Through Co-expression Gene Network and Comparative Genomics Analyses

et al. 2020

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“…Sorghum leaves constitute sucrose known to be translocated and transformed into starch during the development of grain (Smith and Frederiksen 2000). A mutant, named RED for GREEN (RG) displayed increased accumulation of lignin and reduced saccharification efficiency in leaves with concomitant depletion in the stems suggesting that the red leaf coloration of the RG mutant represents a potential marker for improved conversion of stem cellulose to fermentable sugars in the C 4 grass sorghum (Petti et al 2013).…”

Section: Bioconversion Efficiencymentioning

confidence: 99%

Sweet sorghum ideotypes: genetic improvement of the biofuel syndrome

Anami

Zhang

Xia

et al. 2015

Food and Energy Security

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Compared to other potential feedstocks such as sugarcane, sugar beet, maize, and watermelon, sweet sorghum possesses higher levels of directly fermentable reducing sugars within the culm and the ability to accumulate high biomass under low-input production systems. In addition, it is tolerant to drought and has more efficient utilization of solar radiation and nitrogen-based fertilizers than maize and sugar cane on marginal lands that are not optimal for food production. These collectively make sweet sorghum to be considered with huge potential as a biofuel crop. Novel phenotypes generated during plant domestication and continued crop improvements via artificial selection constitute the domestication syndrome (Am. J. Bot., 101, 2014, 1711). Here, we draw an analogy and introduce the term the biofuel syndrome to refer to a suite of sweet sorghum traits, such as plant architecture (root, leave, and stem), flowering time and maturity as well as biomass bioconversion efficiency, that are associated with biofuel production and distinguish it from grain and forage sorghum traits. We discuss the biofuel syndrome amenable for targeted genetic modulation and what is currently known about the genetics and genomics of these traits as a potential route to optimize sweet sorghum for biofuel production. Continuous availability of sweet sorghum, transport and storing much mass and minimizing the postharvest loss of fermentable sugars are fundamental to exploiting sweet sorghum as a bioenergy crop. Due to the relatively short history of sweet sorghum breeding, we consider the development of ideotypes adapting to various phenological requirements to maximize the rapid deployment of sweet sorghum for biofuel production.

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Sorghum mutant RGdisplays antithetic leaf shoot lignin accumulation resulting in improved stem saccharification properties

Cited by 24 publications

References 72 publications

Genotype, development and tissue-derived variation of cell-wall properties in the lignocellulosic energy crop Miscanthus

Genotype, development and tissue-derived variation of cell-wall properties in the lignocellulosic energy crop Miscanthus

Transcriptional Regulation of Sorghum Stem Composition: Key Players Identified Through Co-expression Gene Network and Comparative Genomics Analyses

Sweet sorghum ideotypes: genetic improvement of the biofuel syndrome

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