Sorbitol dehydrogenase is a cytosolic protein required for sorbitol metabolism in Arabidopsis thaliana

Aguayo, M; Ampuero, Diego; Mandujano, Patricio; Parada, Roberto Pantoja; Muñoz, Rodrigo A.A.; Gallart, Marta; Altabella, Teresa; Cabrera, Ricardo; Stange, Claudia; Handford, Michael

doi:10.1016/j.plantsci.2013.01.012

Cited by 41 publications

(43 citation statements)

References 66 publications

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“…Although sorbitol dehydrogenase has been studied more intensively in other species such as pear, an NAD-dependent Arabidopsis enzyme has recently been characterized (Nosarzewski et al, 2012;Aguayo et al, 2013). Interestingly, this enzyme can oxidize other sugar alcohols as well as sorbitol, including ribitol, and the increase in ribose in cat2 (produced, perhaps, from its isomer, ribulose) could be partly linked to this enhanced activity of this enzyme.…”

Section: Sugars and Sugar Alcoholsmentioning

confidence: 99%

The metabolomics of oxidative stress

2015

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Section: Sugars and Sugar Alcoholsmentioning

confidence: 99%

The metabolomics of oxidative stress

2015

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“…), while Arabidopsis plants lacking SDH expression are much more tolerant to drought, with reduced ability to degrade sorbitol under short day conditions (8 h light/16 h dark photoperiod) (Aguayo et al . ). The regulatory mechanism of SDH in response to abiotic stress is complex and remains to be explored.…”

Section: Introductionmentioning

confidence: 97%

“…; Aguayo et al . , ). More than 90 SDH genes have been identified from 42 angiosperm species, such as apple, pear, peach, maize, tomato and Arabidopsis (Doehlert et al .…”

Section: Introductionmentioning

confidence: 99%

Overexpression of SDH confers tolerance to salt and osmotic stress, but decreases ABA sensitivity in Arabidopsis

Shi

Ren

et al. 2017

Plant Biol J

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Sorbitol dehydrogenase (SDH) catalyses the reversible oxidation of sorbitol, xylitol and ribitol to their corresponding ketoses. In this study, we investigated the expression and role of Arabidopsis SDH in salt and osmotic stress tolerance, and abscisic acid (ABA) response. The expression patterns of SDH were investigated using transgenic Arabidopsis plants expressing beta-glucuronidase (GUS) under control of the promoter with the first intron of SDH. qRT-PCR and histochemical assay of GUS activity were used to study SDH expression regulation by ABA, salt and osmotic stress. SDH-overexpression lines of Arabidopsis were used to investigate the role of SDH in salt and osmotic stress, and ABA response. Arabidopsis SDH was predominantly expressed in source organs such as green cotyledons, fully expanded leaves and sepals, especially in vascular tissues of theses organs. SDH expression was inhibited by NaCl and mannitol treatments. Seed germination and post-germination growth of SDH-overexpressing lines exhibited decreased sensitivity to salt and osmotic stress compared to WT plants. The transcript of SDH was induced by ABA. Overexpression of SDH decreased sensitivity to ABA during seed germination and post-germination growth. Expression of AAO3 increased but ABI5 and MYB2 decreased in SDH-overexpressing lines after ABA treatment. This study demonstrates that expression of SDH is regulated by ABA, salt and osmotic stress. SDH functions in plant tolerance to salt and osmotic stress, and ABA response via specific regulating gene expression of ABA synthesis and signalling in Arabidopsis.

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“…The root-specific rice catB promoter or the wheat endosperm-specific Glu-1Bx17 HMW GS promoter fused to the first intron of the rice actin gene (already tested in rice) may serve as alternatives [123,124]. SDH enzymes convert sorbitol to fructose and produce NADH [125][126][127][128]. The uptake of sorbitol to rice grain and root cortex may require the seed and root-specific expression of the apple MdSOT3 inward sorbitol transporter as well [129].…”

Section: Sugar Alcohol Metabolismmentioning

confidence: 99%

Current and possible approaches for improving photosynthetic efficiency

2019

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One of the most important tasks laying ahead today's biotechnology is to improve crop productivity with the aim of meeting increased food and energy demands of humankind. Plant productivity depends on many genetic factors, including life cycle, harvest index, stress tolerance and photosynthetic activity. Many approaches were already tested or suggested to improve either. Limitations of photosynthesis have also been uncovered and efforts been taken to increase its efficiency. Examples include decreasing photosynthetic antennae size, 2 increasing the photosynthetically available light spectrum, countering oxygenase activity of Rubisco by implementing C4 photosynthesis to C3 plants and altering source to sink transport of metabolites. A natural and effective photosynthetic adaptation, the sugar alcohol metabolism got however remarkably little attention in the last years, despite being comparably efficient as C4, and can be considered easier to introduce to new species. We also propose root to shoot carbon-dioxide transport as a means to improve photosynthetic performance and drought tolerance at the same time. Different suggestions and successful examples are covered here for improving plant photosynthesis as well as novel perspectives are presented for future research.

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Sorbitol dehydrogenase is a cytosolic protein required for sorbitol metabolism in Arabidopsis thaliana

Cited by 41 publications

References 66 publications

The metabolomics of oxidative stress

The metabolomics of oxidative stress

Overexpression of SDH confers tolerance to salt and osmotic stress, but decreases ABA sensitivity in Arabidopsis

Current and possible approaches for improving photosynthetic efficiency

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