Sorbitol and Gluconate Production in a Hollow Fibre Membrane Reactor by Immobilized <i>Zymomonas Mobilis</i>

Paterson, Sam; Fane, Anthony G.; Fell, C.J.D.; Chun, Uck-Han; Rogers, Peter L.

doi:10.3109/10242428809014848

Cited by 33 publications

(8 citation statements)

References 7 publications

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“…In contrast, the Km of CTAB-treated cells is significantly lower than a Km of 250 g. 1-1 reported for toluene-permeabilized cells (Paterson et al 1988). For the purified glucose-fructose oxidoreductase a Km of 72 g. 1-1 for fructose and a Kn~ of 1.8 g" 1-1 for glucose were calculated, indicating that fructose makes the main contribution to the high gm obtained with the permeabilized cells (Hardmann and Scopes 1988;Paterson et al 1988). Due to the high K,~ of the glucose-fructose oxidoreductase for fructose a high conversion of glucose/fructose mixtures ( > 98% conversion) in a continuous process can only be obtained at long residence times or in a multi-stage reactor system.…”

Section: Continuous Production Of Sorbitol and Oluconate In Fluidizedmentioning

confidence: 52%

“…g -l . h -i (Barrow et al 1984;Paterson et al 1988). In contrast, the Km of CTAB-treated cells is significantly lower than a Km of 250 g. 1-1 reported for toluene-permeabilized cells (Paterson et al 1988).…”

Section: Continuous Production Of Sorbitol and Oluconate In Fluidizedmentioning

confidence: 99%

“…h -i (Barrow et al 1984;Paterson et al 1988). In contrast, the Km of CTAB-treated cells is significantly lower than a Km of 250 g. 1-1 reported for toluene-permeabilized cells (Paterson et al 1988). For the purified glucose-fructose oxidoreductase a Km of 72 g. 1-1 for fructose and a Kn~ of 1.8 g" 1-1 for glucose were calculated, indicating that fructose makes the main contribution to the high gm obtained with the permeabilized cells (Hardmann and Scopes 1988;Paterson et al 1988).…”

Section: Continuous Production Of Sorbitol and Oluconate In Fluidizedmentioning

confidence: 99%

“…No significant gluconate production was observed, since in contrast to sorbitol the gluconic acid produced was phosphorylated by the gluconatekinase and further metabolized in the Entner-Doudoroff pathway to ethanol (Barrow et al 1984;Zachariou and Scopes 1985;Strohdeicher et al 1988). Studies with toluene-treated, permeabilized cells showed that glucose-fructose mixtures up to 60% (w/v) can be converted to 283 g/1 of gluconic acid and 290 g. 1-1 of sorbitol, respectively Chun and Rogers 1988;Paterson et al 1988). The permeability of the cell membrane was increased by the toluene treatment and soluble cofactors necessary for the fermentation of the sugars and the gluconic acid to ethanol were removed ).…”

Section: Introductionmentioning

confidence: 99%

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Production of sorbitol and gluconic acid by permeabilized cells of Zymomonas mobilis

Rehr

Wilhelm

Sahm

1991

Appl Microbiol Biotechnol

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Section: Continuous Production Of Sorbitol and Oluconate In Fluidizedmentioning

confidence: 52%

Section: Continuous Production Of Sorbitol and Oluconate In Fluidizedmentioning

confidence: 99%

Section: Continuous Production Of Sorbitol and Oluconate In Fluidizedmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Production of sorbitol and gluconic acid by permeabilized cells of Zymomonas mobilis

Rehr

Wilhelm

Sahm

1991

Appl Microbiol Biotechnol

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“…[4 -6] On one hand, production of gluconic acid has utilized chemical, electrochemical, and bioelectrochemical approaches. [6 -12] On the other hand, biochemical strategies to oxidize glucose into gluconic acid relied on enzymes such as Aspergillus niger, [8] Penicillium sp., [9] Zymomonas mobilis, [10] and Gluconobacter oxydans, or cells. [11 -12] The use of microwave (MW) technology has been reported to cause a dramatic decrease in reaction time and possibly enhance the regio-and stereoselectivities in organic reactions.…”

Section: Introductionmentioning

confidence: 99%

MAOS ofD‐Gluconic Acid,D‐Glucono‐1,4‐ and 1,5‐Lactones, Esters, Hydrazides, and Benzimidazoles Thereof

Ashry

Awad

Hamid

et al. 2007

Journal of Carbohydrate Chemistry

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Microwave-assisted organic synthesis (MAOS) of D-gluconic acid can be efficiently done by oxidation of D-glucose with bromine water, upon irradiation with microwave (MW). It was also used for the conversion of D-gluconic acid to ethyl D-gluconate, D-glucono-1,4-and 1,5-lactones, gluconyl hydrazide, and gluconyl phenylhydrazide in yields comparable to those obtained by conventional methods, but in much shorter times. A convenient microwave-mediated condensation of D-gluconic acid with o-phenylenediamines provided the respective acyclonucleoside benzimidazole in short time and good yield.

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Improved operational stability of cell-free glucose-fructose oxidoreductase fromZymomonas mobilis for the efficient synthesis of sorbitol and gluconic acid in a continuous ultrafiltration membrane reactor

Nidetzky

Fürlinger

Gollhofer

et al. 1997

Biotechnol. Bioeng.

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For the continuous, enzymatic synthesis of sorbitol and gluconic acid by cell‐free glucose‐fructose oxidoreductase (GFOR) from Zymomonas mobilis, the principal determinants of productivity have been identified. Most important, the rapid inactivation of the soluble enzyme during substrate conversion can be avoided almost completely when weak bases such as tris(hydroxymethyl)aminomethan or imidazol are used for the titration of the produced gluconic acid and when 5–10 mM dithiothreitol are added to prevent thiol oxidations. With regard to a long‐term operational stability of the enzyme for continuous syntheses, thermal deactivation becomes significant at reaction temperatures above 30°C. Without any additional purification being required, the crude cell extract of Z. mobilis can be employed in a continuous ultrafiltration membrane reactor over a time period of more than 250 h without significant decrease in substrate conversion or enzyme activity. The use of soluble GFOR thus appears to be an interesting alternative to employing permeabilized cells of Zymomonas for the production of sorbitol and gluconic acid and may be superior with regard to reactor productivities, at comparable operational stabilities. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 53: 623–629, 1997.

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Sorbitol and Gluconate Production in a Hollow Fibre Membrane Reactor by Immobilized Zymomonas Mobilis

Cited by 33 publications

References 7 publications

Production of sorbitol and gluconic acid by permeabilized cells of Zymomonas mobilis

Production of sorbitol and gluconic acid by permeabilized cells of Zymomonas mobilis

MAOS ofD‐Gluconic Acid,D‐Glucono‐1,4‐ and 1,5‐Lactones, Esters, Hydrazides, and Benzimidazoles Thereof

Improved operational stability of cell-free glucose-fructose oxidoreductase fromZymomonas mobilis for the efficient synthesis of sorbitol and gluconic acid in a continuous ultrafiltration membrane reactor

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