Sophocarpine can enhance the inhibiting effect of oxaliplatin on colon cancer liver metastasis—in vitro and in vivo

Yang, Yu-Shen; Wen, Dan; Zhao, Xue-Feng

doi:10.1007/s00210-020-02032-8

Cited by 6 publications

(5 citation statements)

References 40 publications

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“… Liu et al (2017) found that in head and neck cancer, sophocarpine upregulated E-cadherin expression (epithelial marker) and decreased vimentin expression (mesenchymal marker). Yang, Wen & Zhao (2021) demonstrated that in colon cancer cells, sophocarpine reversed the EMT process by upregulating the expression of E-cadherin and downregulating the expression of N-cadherin and vimentin. All these results suggested that sophocarpine might inhibit cell migration and invasion by inhibiting EMT.…”

Section: Discussionmentioning

confidence: 99%

Sophocarpine inhibits tumor progression by antagonizing the PI3K/AKT/mTOR signaling pathway in castration-resistant prostate cancer

Wang

Shi

Han

et al. 2022

PeerJ

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Objective The objective of this study was to investigate the inhibitory effect of sophocarpine on the progression of castration-resistant prostate cancer (CRPC) and the underlying molecular mechanism. Methods DU145 and PC3 cells (two CRPC cell lines), incubated with different concentrations of sophocarpine, were used. Cell Counting Kit-8 assay, real-time cellular analysis, and colony formation assay were conducted to evaluate the proliferation of CRPC cells. Cytometry flow analysis was performed to evaluate the apoptosis rate of CRPC cells. Wound healing and Transwell invasion assays were performed and the levels of the epithelial-mesenchymal transition (EMT)-related proteins were determined to analyze cell migration and invasion abilities. A xenografted tumor model of nude mice was used to examine the anti-cancer effect of sophocarpine on CRPC. Western blotting was performed to evaluate the activities of the PI3K/AKT/mTOR signaling pathway both in cells and tumor tissues. Results In vitro tests showed that sophocarpine suppressed the proliferation of CRPC cells, reduced the migration and invasion abilities, and increased the apoptosis rate. In vivo, sophocarpine decreased the weight and volume of tumor tissues. Mechanically, sophocarpine exerted its anti-cancer effects by inactivating PI3K/AKT/mTOR signaling. Conclusion Sophocarpine inhibited the progression of CRPC by downregulating the PI3K/AKT/mTOR signaling pathway and showed a potential to be an anti-cancer agent against CRPC.

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Section: Discussionmentioning

confidence: 99%

Sophocarpine inhibits tumor progression by antagonizing the PI3K/AKT/mTOR signaling pathway in castration-resistant prostate cancer

Wang

Shi

Han

et al. 2022

PeerJ

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“…A biologically active chemical alkaloid, sophocarpine, is separated from Compound kushen injection (Zhang et al, 2018). As reported before, sophocarpine might be a potential broad-spectrum anticancer agent (Zhang et al, 2016;Huang et al, 2019;Wang et al, 2019;Yang et al, 2021). What's more, sophocarpine also inhibited CYP3A4 noncompetitively and CYP2C9 competitively in human liver microsomes (Zhang et al, 2019).…”

Section: Introductionmentioning

confidence: 87%

Effect of Sophocarpine on the Pharmacokinetics of Umbralisib in Rat Plasma Using a Novel UPLC-MS/MS Method

et al. 2022

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Umbralisib is a dual inhibitor of phosphatidylinositol 3-kinase delta (PI3Kδ) and casein kinase 1 epsilon (CK1ε) for treating marginal zone lymphoma (MZL) and follicular lymphoma (FL). This study aimed to develop a fast and stable ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for quantitative analysis of umbralisib in rat plasma and its application for evaluating the effect of sophocarpine on the pharmacokinetics of umbralisib. A direct protein preparation with acetonitrile was used to deal with rat plasma. Umbralisib and duvelisib (internal standard, IS) were isolated on a Waters Acquity UPLC BEH C18 column with mobile phase consisted of acetonitrile and 0.1% formic acid in water. The linear range was from 0.5 to 1,000 ng/ml. Both of the precision (RSD%) and accuracy (RE%) were less than 15% in a permissible range. The mean recovery and matrix effect of umbralisib were 86.3–96.2% and 97.8–112.0%, respectively. When umbralisib was combined with sophocarpine, AUC0→∞ of umbralisib was significantly reduced to 2462.799 ± 535.736 ng/ml•h from 5416.665 ± 1,451.846 ng/ml•h, and Cmax also was markedly diminished. Moreover, CLz/F was increased more than two times. This developed, optimized and technical UPLC-MS/MS method was extremely suitable for detecting the concentrations of umbralisib in rat plasma after an oral administration, and sophocarpine significantly changed the pharmacokinetics of umbralisib in rats. This obvious pharmacokinetic changes indicates that there seems to exist herb-drug interaction between sophocarpine and umbralisib.

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“…Through the CDX model, our group found that the traditional Chinese medicine sophocarpine can enhance the inhibitory effect of oxaliplatin on metastatic CRC 73 . Additionally, in the KRAS-mutated metastatic CRC xenografted model, metformin was reported to inhibit tumour growth and cell viability 95 .…”

Section: Drug Testingmentioning

confidence: 99%

“…After intrasplenic injection, tumour cells directly enter the bloodstream and then reach the liver, where they proliferate towards secondary lesions, which prevents them from undergoing the initial stage of metastasis. These characteristics make the intrasplenic transplantation model irreplaceable in the study of human tumour liver metastasis 73 .…”

Section: Intrasplenic Transplantationmentioning

confidence: 99%

Recent advances in the development of transplanted colorectal cancer mouse models

Yang

Liu

Wen

et al. 2022

Translational Research

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Sophocarpine can enhance the inhibiting effect of oxaliplatin on colon cancer liver metastasis—in vitro and in vivo

Cited by 6 publications

References 40 publications

Sophocarpine inhibits tumor progression by antagonizing the PI3K/AKT/mTOR signaling pathway in castration-resistant prostate cancer

Sophocarpine inhibits tumor progression by antagonizing the PI3K/AKT/mTOR signaling pathway in castration-resistant prostate cancer

Effect of Sophocarpine on the Pharmacokinetics of Umbralisib in Rat Plasma Using a Novel UPLC-MS/MS Method

Recent advances in the development of transplanted colorectal cancer mouse models

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