Sonoporation: Applications for Cancer Therapy

Qin, Jiale; Wang, Tzu-Yin; Willmann, Jürgen K.

doi:10.1007/978-3-319-22536-4_15

Cited by 53 publications

(44 citation statements)

References 114 publications

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“…Lastly, a completely separate and additional advantage of such LCM/ND lipid nanoemulsion(s), as a component of this combination therapeutic, stems from the characteristic lipid-coated microbubble subpopulation (D'Arrigo 2011) existing in this nanoemulsion type. Specifically, such preformed (lipidstabilized) microbubbles are well known to substantially reduce the acoustic power levels needed for accomplishing temporary noninvasive (transcranial) ultrasound treatment Alonso et al 2010;Alonso et al 2011;Aslund et al 2017;Bing et al 2014;D'Arrigo 2015;Delalande et al 2013;Goliaei et al 2015;Kotopoulis et al 2013;Kotopoulis et al 2014;Lammers et al 2015;Marquet et al 2011 O' Reilly et al 2017;Paefgen et al 2015;Qin et al 2016;Sennoga et al 2016), if additionally desired for the Alzheimer's patient.…”

Section: Resultsmentioning

confidence: 99%

Nanotherapy for Alzheimer's disease and vascular dementia: Targeting senile endothelium

D'Arrigo

2018

Advances in Colloid and Interface Science

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Due to the complexity of Alzheimer's disease, multiple cellular types need to be targeted simultaneously in order for a given therapy to demonstrate any major effectiveness. Ultrasound-sensitive coated microbubbles (in a targeted lipid nanoemulsion) are available. Versatile small molecule drug(s) targeting multiple pathways of Alzheimer's disease pathogenesis are known. By incorporating such drug(s) into the targeted LCM/ND lipid nanoemulsion type, one obtains a multitasking combination therapeutic for translational medicine. This multitasking therapeutic targets cell-surface scavenger receptors (mainly SR-BI), making possible for various Alzheimer's-related cell types to be simultaneously searched out for localized drug treatment in vivo. Besides targeting cell-surface SR-BI, the proposed LCM/ND-nanoemulsion combination therapeutic(s) include a characteristic lipid-coated microbubble [LCM] subpopulation (i.e., a stable LCM suspension); such filmstabilized microbubbles are well known to substantially reduce the acoustic power levels needed for accomplishing temporary noninvasive (transcranial) ultrasound treatment, or sonoporation, if additionally desired for the Alzheimer's patient.

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Section: Resultsmentioning

confidence: 99%

Nanotherapy for Alzheimer's disease and vascular dementia: Targeting senile endothelium

D'Arrigo

2018

Advances in Colloid and Interface Science

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“…Lastly, a completely separate and additional advantage of such LCM/ND lipid nanoemulsion(s), as a component of this combination therapeutic, stems from the characteristic lipid-coated microbubble subpopulation (D'Arrigo 2011) existing in this nanoemulsion type. Specifically, such preformed (lipid-stabilized) microbubbles are well known to substantially reduce the acoustic power levels needed for accomplishing temporary noninvasive (transcranial) ultrasound treatment (Adhikari et al 2015;Alonso et al 2010;Alonso et al 2011;Aslund et al 2017;Bing et al 2014;D'Arrigo 2015;Delalande et al 2013;Goliaei et al 2015;Kotopoulis et al 2013;Kotopoulis et al 2014;Lammers et al 2015;Marquet et al 2011;Meairs 2015;Meng et al 2017;Miller and O'Callaghan 2017), or sonoporation (Aubry et al 2016;Bouakaz et al 2016;Burgess and Hynynen 2016;Castle and Feinstein 2016;Delalande et al 2015;Horodyckid et al 2016;O'Reilly et al 2017;Paefgen et al 2015;Qin et al 2016;Sennoga et al 2016), if additionally desired for the Alzheimer's patient.…”

Section: Resultsmentioning

confidence: 99%

Nanotherapy for Early Dementia: Targeting Senile Endothelium

D'Arrigo

2017

Preprint

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Due to the complexity of Alzheimer's disease, multiple cellular types need to be targeted simultaneously in order for a given therapy to demonstrate any major effectiveness. Ultrasound-sensitive coated microbubbles (in a targeted lipid nanoemulsion) are available. Versatile small molecule drug(s) targeting multiple pathways of Alzheimer's disease pathogenesis are known. By incorporating such drug(s) into the targeted LCM/ND lipid nanoemulsion type, one obtains a multitasking combination therapeutic for translational medicine. This multitasking therapeutic targets cell-surface scavenger receptors (mainly SR-BI), making possible for various Alzheimer's-related cell types to be simultaneously searched out for localized drug treatment in vivo. Besides targeting cell-surface SR-BI, the proposed LCM/ND-nanoemulsion combination therapeutic(s) include a characteristic lipid-coated microbubble [LCM] subpopulation (i.e., a stable LCM suspension); such film-stabilized microbubbles are well known to substantially reduce the acoustic power levels needed for accomplishing temporary noninvasive (transcranial) ultrasound treatment, or sonoporation, if additionally desired for the Alzheimer's patient.

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“…Various studies have proved that UTMD suppresses the progression of carcinoma, and sonoporation-induced biological barrier permeability has emerged as a pivotal mechanism of UTMD in tumor progression repression 9,14,25 . To explore whether there is any other mechanism of UTMD-inhibited tumor progression, we investigated the role of UTMD in inhibiting breast cancer EMT and tried to reveal its mechanism.…”

Section: Discussionmentioning

confidence: 99%

UTMD inhibit EMT of breast cancer through the ROS/miR-200c/ZEB1 axis

Shi

Guo

Sun

et al. 2020

Sci Rep

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As a potential drug/gene delivery system, the ultrasound-targeted microbubble destruction (UtMD) system can be used as a vehicle as well as increasing the permeability of biological barriers to enhance the effect of tumor treatment. However, the effect of UTMD in the tumor EMT process is unknown. In this study, we aimed to investigate the potential and mechanism of UtMD induced oxidative stress in inhibiting EMT of breast cancer. Human breast MDA231 cells were treated with microbubble (MB), ultrasound (US) and UTMD, respectively. The generation of oxidative stress, the levels of miR-200c, ZEB1 and vimentin, and the numbers of migratory cells were evaluated quantitatively and qualitatively by the measurement of intracellular reactive oxygen species (ROS), qRT-PCR, western blot assay, and transwell assay. Then, to evaluate the role of UTMD-induced oxidative stress and miR-200c in the epithelial-mesenchymal transition (eMt) inhibition, the RoS scavenger n-acetyl-L-cysteine (nAc) and miR-200c inhibitor were used before UTMD treatment. We found that UTMD induced oxidative stress, upregulated the expression of miR-200c, downregulated the expression of ZEB1 and vimentin and suppressed the MDA231 cell migration. The addition of NAC and miR-200c inhibitor had an opposite impact on the expression of miR-200c and ZEB1, thus hindered the effects of UTMD on MDA231 cells EMT. In conclusion, UTMD can inhibit the EMT characteristics of MDA231 cells. The mechanism may be related to the regulation of the miR-200c/ZEB1 axis through the generation of ROS induced by UTMD, which may provide a new strategy to prevent the tumor cells EMT under UTMD treatment. The metastasis of cancer is a complicated process, and is the main cause of the patients' death. Epithelial-mesenchymal transition (EMT) is a key regulator of aggressive invasion and metastasis in tumorigenesis 1,2. During the EMT process, the epithelial cells lose the apical-basal polarity and cell-cell adhesion and get mesenchymal characteristics 3. The major EMT markers comprise epithelial markers, such as E-cadherin, and mesenchymal markers, such as vimentin, N-cadherin, and fibronectin. Zinc finger E-box binding homeobox transcription factor 1 (ZEB1) is a critical EMT-related transcription factor (EMT-TF). ZEB1 knockdown represses vimentin expression, upregulated E-cadherin expression, and inhibited cell proliferation and metastasis 4. Another central regulator of EMT is the microRNA-200 (miR-200) family, which consists of 5 members (miR-200a,-200b,-200c,-141 and-429) 5. Mounting evidence suggests that ZEB1 and miR-200c reciprocally control their expression through a negative regulatory loop, and miR-200c repression or ZEB1 expression has been associated with a worse prognosis in several carcinomas, such as breast and ovarian cancer 5-7. Ultrasound (US) imaging is a real-time imaging technique which allows visualization of organ structure. Microbubbles (MBs) are used as ultrasonic contrast agents which increase the image contrast due to the acoustic mismatch between the gas an...

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Sonoporation: Applications for Cancer Therapy

Cited by 53 publications

References 114 publications

Nanotherapy for Alzheimer's disease and vascular dementia: Targeting senile endothelium

Nanotherapy for Alzheimer's disease and vascular dementia: Targeting senile endothelium

Nanotherapy for Early Dementia: Targeting Senile Endothelium

UTMD inhibit EMT of breast cancer through the ROS/miR-200c/ZEB1 axis

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