Sonochemical synthesis of polyoxometalate-stabilized gold nanoparticles for point-of-care determination of acetaminophen levels: preclinical study in an animal model

Abstract

The aim of this study is the accurate detection of acetaminophen (AP) for point-of-care (POC) clinical diagnosis. The concentration of acetaminophen was measured in over-the-counter pharmaceutical tablets and in serum samples taken from mice.

AuNPs@PMo12nanozyme: highly oxidase mimetic activity for sensitive and specific colorimetric detection of acetaminophen

“…According to previous research, AuNPs@PMo 12 nanohybrid is aggregated in the presence of Au(III) and AP. 32 As seen in Fig. 4, by adding AP into AuNPs@PMo 12 solution some islands of AuNPs aggregates were seen.…”

“…The synthesis pathway was according to the previous study. 32 Briey, the HAuCl 4 solution (1 mM, 16.5 mL) and PMo 12 (1 mM, 33 mL) were mixed and stirred at room temperature. Aer that, the resulting solution was transferred into 100 mL Dewar cell, and 1.2 mL of propanol was added into this solution.…”

“…As mentioned in the previous study, Au(III) ions interacted with the -OH group of AP (C 8 H 9 NO 2 ) molecules via donoracceptor electron complex. 32 Besides, AP molecules with a functional group (-NH) form a hydrogen bonding and donoracceptor electron complexes into the AuNPs@PMo 12 surface. Therefore, AP molecules in the presence of Au(III) can induce the AuNPs@PMo 12 aggregation.…”

“…Therefore, AP molecules in the presence of Au(III) can induce the AuNPs@PMo 12 aggregation. 32 Thus, fewer amounts of active sites for the oxidation of TMB were accessible and the complete oxidation of TMB was prevented. Fig.…”

“…In the present research, a nanozyme, polyoxometalatestabilized gold nanoparticles (AuNPs@PMo 12 ) was prepared under ultrasonic irradiation according to the previous literature. 32 Sono-synthesized samples were kept at room temperature and remained stable for more than three months. The asprepared nanosensor showed remarkable oxidase-like activity of colourless TMB into the coloured product in the free H 2 O 2 medium.…”