Sonication-assisted Agrobacterium-mediated transformation of the ACC gene to interfere the production of ethylene in spring Dendrobium cv. ‘Sanya’

Zheng, Qi; Zheng, Y. G.; Wang, G. D.; Guo, Wenshan; Fan, Wei; Wang, C.

doi:10.1134/s1021443712010190

Cited by 7 publications

(2 citation statements)

References 17 publications

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“…This indicated that the pericarp can be resistant to Agrobacterium infection into the endosperm and embryo of husked seeds because the pericarp has a hard structure consisting of high cellulose, hemicellulose and lignin contents [15]. Moreover, this result may indicate that Agrobacterium can infect the dehusked seeds which causes microwounds within and on the tissue [16], inducing phenolic-compound synthesis [17] and vacuum infiltration might allow better access and infection of plant cells by Agrobacterium [8].…”

Section: Effect Of Husked and Dehusked Mature Seeds Of Rice On Gene Tmentioning

confidence: 99%

Application of sonication- and vacuum infiltration-assisted Agrobacterium-mediated transformation of rice embryo (Oryza sativa L.)

Jongjitvimol¹,

Sujipuli²,

Urtgam³

2020

ScienceAsia

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Section: Effect Of Husked and Dehusked Mature Seeds Of Rice On Gene Tmentioning

confidence: 99%

Application of sonication- and vacuum infiltration-assisted Agrobacterium-mediated transformation of rice embryo (Oryza sativa L.)

Jongjitvimol¹,

Sujipuli²,

Urtgam³

2020

ScienceAsia

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“…Based on these studies, virus resistant plants were successfully produced by introducing viral coat proteins in the genome of Dendrobium (Chang et al 2005). The inhibition of ethylene production in order to delay the senescence and increase the flower longevity in Dendrobium has also been reported recently (Zheng et al 2012).…”

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confidence: 99%

<i>Agrobacterium</i>-mediated transformation of protocorm-like bodies in <i>Dendrobium</i> Formidible ‘Ugusu’

Phlaetita

Chin

Tokuhara

et al. 2015

Plant Biotechnology

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Agrobacterium-mediated genetic transformation system was established in Dendrobium Formidible 'Ugusu' by inoculating PLBs with A. tumefaciens strain EHA101 (pIG121Hm) harboring hygromycin phosphotranferase (hpt) and neomycin phosphotranferase II (nptII) genes as selectable marker gene and β-glucuronidase (gus) gene as a reporter gene. For obtaining the optimum conditions for the transformation, several factors such as the stage of PLBs after subculture, bacterial concentrations, kind of inoculation medium, inoculation time and inoculation condition (with or without rotary shaking), were examined. After inoculation, PLBs were cocultivated for 3 days and then transferred for selection onto 2.5 g l −1 gellan gum-solidified ND medium containing 10 g l −1 maltose, 20 mg l −1 hygromycin and 20 mg l −1 meropenem. Hygromycin-resistant plantlets were regenerated from secondary PLBs after 4 months of selection. Transformation of these plants was confirmed by GUS histochemical assay, PCR and Southern blot analyses. The highest transformation efficiency of 18.5% was obtained when PLBs 3 weeks after subculture were inoculated with 1 : 10 diluted bacteria (OD 600 ≈0.1) with liquid medium containing only 10 g l −1 maltose and 100 µM acetosyringone with shaking for 30 min.

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Methods for genetic transformation in Dendrobium

Silva

Dobránszki

Cardoso³

et al. 2016

Plant Cell Rep

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The genetic transformation of Dendrobium orchids will allow for the introduction of novel colours, altered architecture and valuable traits such as abiotic and biotic stress tolerance. The orchid genus Dendrobium contains species that have both ornamental value and medicinal importance. There is thus interest in producing cultivars that have increased resistance to pests, novel horticultural characteristics such as novel flower colours, improved productivity, longer flower spikes, or longer post-harvest shelf-life. Tissue culture is used to establish clonal plants while in vitro flowering allows for the production of flowers or floral parts within a sterile environment, expanding the selection of explants that can be used for tissue culture or genetic transformation. The latter is potentially the most effective, rapid and practical way to introduce new agronomic traits into Dendrobium. Most (69.4 %) Dendrobium genetic transformation studies have used particle bombardment (biolistics) while 64 % have employed some form of Agrobacterium-mediated transformation. A singe study has explored ovary injection, but no studies exist on floral dip transformation. While most of these studies have involved the use of selector or reporter genes, there are now a handful of studies that have introduced genes for horticulturally important traits.

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Sonication-assisted Agrobacterium-mediated transformation of the ACC gene to interfere the production of ethylene in spring Dendrobium cv. ‘Sanya’

Cited by 7 publications

References 17 publications

Application of sonication- and vacuum infiltration-assisted Agrobacterium-mediated transformation of rice embryo (Oryza sativa L.)

Application of sonication- and vacuum infiltration-assisted Agrobacterium-mediated transformation of rice embryo (Oryza sativa L.)

<i>Agrobacterium</i>-mediated transformation of protocorm-like bodies in <i>Dendrobium</i> Formidible ‘Ugusu’

Methods for genetic transformation in Dendrobium

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