Sonic hedgehog signaling in the development of the mouse hypothalamus

Blaess, Sandra; Szabó, Nora; Haddad-Tóvolli, Roberta; Zhou, Xunlei; Álvarez-Bolado, Gonzalo

doi:10.3389/fnana.2014.00156

Cited by 40 publications

(43 citation statements)

References 53 publications

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“…Generation of JBST neurons from iPSCs. We attempted to generate ARH-like neurons from the JBST iPSCs but were unable to generate NKX2.1-positive neuronal progenitors from which POMC and NPY neurons derive (38,39), further suggesting the importance of RPGRIP1L in the development of ARH neurons. Subsequently, we utilized a dual-SMAD inhibition protocol (40) to direct differentiation of the JBST iPSCs into neurons positive for neuromarkers Nestin, neuron-specific class III β-tubulin (TUJ1), and Neurofilament (NF) (Supplemental Figure 3C).…”

Section: Diminished Ciliation Of Ipsc-derived Neurons Of Patients Witmentioning

confidence: 99%

Ciliary gene RPGRIP1L is required for hypothalamic arcuate neuron development

et al. 2019

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Section: Diminished Ciliation Of Ipsc-derived Neurons Of Patients Witmentioning

confidence: 99%

Ciliary gene RPGRIP1L is required for hypothalamic arcuate neuron development

et al. 2019

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“…Shh, which is secreted by prechordal mesoderm at E7.5 and by hypothalamic neuroepithelium at E8.5, plays a critical role in the development of the ventral portion of the neural tube (10). Shh signaling increases Nkx2-1 expression (11), which, as mentioned above, plays a critical role in the development of the neural tube ventral midline (9), and deletion of Shh in mice with the Nkx2-1-Cre driver (12) affects the development of POMC neurons.…”

Section: Pomc Progenitor/lineage and Adult Neurogenesismentioning

confidence: 99%

POMC Neurons: From Birth to Death

Toda

Santoro

Kim

et al. 2017

Annu. Rev. Physiol.

128

121

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The hypothalamus is an evolutionarily conserved brain structure that regulates an organism’s basic functions, such as homeostasis and reproduction. Several hypothalamic nuclei and neuronal circuits have been the focus of many studies to understand their role in regulating these basic functions. Within the hypothalamic neuronal populations, the arcuate melanocortin system plays a major role in controlling homeostatic functions. The arcuate pro-opiomelanocortin (POMC) neurons in particular have been shown to be critical regulators of metabolism and reproduction because of their projections to several brain areas both in and outside of the hypothalamus, such as autonomic regions of the brain stem and spinal cord. Here, we review and discuss the current understanding of POMC neurons from their development and intracellular regulators to their physiological functions and pathological dysregulation.

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“…Transcription factors expressed in the ventral forebrain, like Nkx2.1 and Six3, are then necessary to establish the hypothalamic character of the region 3,4 . Shh produced by the ventral forebrain is also necessary to stimulate proliferation and neuronal differentiation in the future hypothalamus 5,6,7,8 .…”

Section: Introductionmentioning

confidence: 99%

Essential function of the transcription factor Rax in the early patterning of the mammalian hypothalamus

Orquera

Nasif

Low

et al. 2016

Developmental Biology

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The hypothalamus is a region of the anterior forebrain that controls basic aspects of vertebrate physiology, but the genes involved in its development are still poorly understood. Here, we investigate the function of the homeobox gene Rax/Rx in early hypothalamic development using a conditional targeted inactivation strategy in the mouse. We found that lack of Rax expression prior to embryonic day 8.5 (E8.5) caused a general underdevelopment of the hypothalamic neuroepithelium, while inactivation at later timepoints had little effect. The early absence of Rax impaired neurogenesis and prevented the expression of molecular markers of the dorsomedial hypothalamus, including neuropeptides Proopiomelanocortin and Somatostatin. Interestingly, the expression domains of genes expressed in the ventromedial hypothalamus and infundibulum invaded dorsal hypothalamic territory, showing that Rax is needed for the proper dorsoventral patterning of the developing medial hypothalamus. The phenotypes caused by the early loss of Rax are similar to those of eliminating the expression of the morphogen Sonic hedgehog (Shh) specifically from the hypothalamus. Consistent with this similarity in phenotypes, we observed that Shh and Rax are coexpressed in the rostral forebrain at late head fold stages and that loss of Rax caused a downregulation of Shh expression in the dorsomedial portion of the hypothalamus.

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Sonic hedgehog signaling in the development of the mouse hypothalamus

Cited by 40 publications

References 53 publications

Ciliary gene RPGRIP1L is required for hypothalamic arcuate neuron development

Ciliary gene RPGRIP1L is required for hypothalamic arcuate neuron development

POMC Neurons: From Birth to Death

Essential function of the transcription factor Rax in the early patterning of the mammalian hypothalamus

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