Something old, something new, something borrowed, something red: the origin of ecologically relevant novelties in Hemiptera

Jockusch, Elizabeth L.; Fisher, Cera R

doi:10.1016/j.gde.2021.04.003

Cited by 8 publications

(8 citation statements)

References 64 publications

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“…Interestingly, v 3 ; Of-xdh1 pRNAi offspring had bright yellow bodies (Fig. 5B), lighter than the v 3 ; gfp pRNAi controls (Fig. 5A), and even lighter than the yellow-orange pigmentation observed after xdh1 pRNAi knockdown in a wild type background (compare to Fig.…”

Section: Xdh1 Knockdown In V 3 Mutant Produces Bright Yellow Body Colormentioning

confidence: 81%

“…From the original five founder crosses, three homozygous Of-v mutant lines were established: v 1 , v 3 , and v 4 . Sequencing of Of-v exon 2 from each line showed all three alleles result in premature stop codons within exon 2 (Fig.…”

Section: Of-vermilion X-linkage and Establishment Of Homozygous Linesmentioning

confidence: 99%

“…The expected genotypes and phenotypes are shown in their expected proportions. (C) Alignment between the wild-type Of-v allele and the three mutant alleles for which homozygous lines were successfully established: v 1 , has a single nucleotide insertion at the predicted double-stranded break site (i), v 3 , which has a 10 bp deletion (ii), and v 4 , which has a 6 bp insertion. Insertions or deletions, blue.…”

Section: Second Of-v Crispr Experimentsmentioning

confidence: 99%

“…Most studies of insects to date have focused on the holometabolous insects, including beetles, butterflies and flies, with Drosophila melanogaster among the most powerful genetic model systems. As a close outgroup to holometabolous insects, the order Hemiptera comprises >80,000 described species 1 that share a piercing-sucking mode of feeding but which are otherwise enormously diverse (reviewed in 2,3 ). This order includes agricultural pests such as aphids and harlequin bugs, nuisance pests of humans such as bed bugs and stink bugs, and vectors of serious human disease, such as kissing bugs, which transmit Chagas disease 4,5 .…”

Section: Introductionmentioning

confidence: 99%

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Genome editing of thevermilionlocus generates a visible eye color marker forOncopeltus fasciatus

Reding

Lê

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2022

Preprint

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Insects display a vast array of eye and body colors. Genes encoding products involved in biosynthesis and deposition of pigments are ideal genetic markers, contributing, for example, to the power of Drosophila genetics. Oncopeltus fasciatus is an emerging model for hemimetabolous insects, a member of the piercing-sucking feeding order Hemiptera, that includes pests and disease vectors. To identify candidate visible markers for O. fasciatus, we used parental and nymphal RNAi to identify genes that altered eye or body color while having no deleterious effects on viability. We selected Of-vermilion for CRISPR/Cas9 genome editing, generating three independent loss-of-function mutant lines. These studies mapped Of-vermilion to the X-chromosome, the first assignment of a gene to a chromosome in this species. Of-vermilion homozygotes have bright red, rather than black, eyes and are fully viable and fertile. We used these mutants to verify a role for Of-xdh1, ortholog of Drosophila rosy, in contributing to red pigmentation after RNAi. Rather than wild-type-like red bodies, bugs lacking both vermilion and xdh1 have bright yellow bodies, suggesting that ommochromes and pteridines contribute to O. fasciatus body color. Our studies generated the first gene-based visible marker for O. fasciatus and expanded the genetic toolkit for this model system.

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Section: Xdh1 Knockdown In V 3 Mutant Produces Bright Yellow Body Colormentioning

confidence: 81%

Section: Of-vermilion X-linkage and Establishment Of Homozygous Linesmentioning

confidence: 99%

Section: Second Of-v Crispr Experimentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome editing of thevermilionlocus generates a visible eye color marker forOncopeltus fasciatus

Reding

Lê

Pick

2022

Preprint

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show abstract

“…Most studies of insects to date have focused on the holometabolous insects, including beetles, butterflies and flies, with Drosophila melanogaster among the most powerful genetic model systems. As a close outgroup to holometabolous insects, the order Hemiptera comprises > 80,000 described species 1 that share a piercing-sucking mode of feeding but which are otherwise enormously diverse (reviewed in 2,3 ). This order includes agricultural pests such as aphids and harlequin bugs, nuisance pests of humans such as bed bugs and brown marmorated stink bugs, and vectors of serious human disease, such as kissing bugs, which transmit Chagas disease 4,5 .…”

mentioning

confidence: 99%

Genome editing of the vermilion locus generates a visible eye color marker for Oncopeltus fasciatus

Reding

Lê

Pick

2023

Sci Rep

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Insects display a vast array of eye and body colors. Genes encoding products involved in biosynthesis and deposition of pigments are ideal genetic markers, contributing, for example, to the power of Drosophila genetics. Oncopeltusfasciatus is an emerging model for hemimetabolous insects, a member of the piercing-sucking feeding order Hemiptera, that includes pests and disease vectors. To identify candidate visible markers for O.fasciatus, we used parental and nymphal RNAi to identify genes that altered eye or body color while having no deleterious effects on viability. We selected Of-vermilion for CRISPR/Cas9 genome editing, generating three independent loss-of-function mutant lines. These studies mapped Of-vermilion to the X-chromosome, the first assignment of a gene to a chromosome in this species. Of-vermilion homozygotes have bright red, rather than black, eyes and are fully viable and fertile. We used these mutants to verify a role for Of-xdh1, ortholog of Drosophilarosy, in contributing to red pigmentation using RNAi. Rather than wild-type-like red bodies, bugs lacking both vermilion and xdh1 have bright yellow bodies, suggesting that ommochromes and pteridines contribute to O.fasciatus body color. Our studies generated the first gene-based visible marker for O.fasciatus and expanded the genetic toolkit for this model system.

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Disruption of transmission of plant pathogens in the insect order Hemiptera using recent advances in RNA interference biotechnology

Niebres,

Alviar

2023

Arch Insect Biochem Physiol

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The review discusses current RNA interference (RNAi) biotechnological innovations for crop protection. Special attention is given to the management of insect pests in the order Hemiptera. This insect order has the most members of insects which transmit pathogens on economically important crops. It first briefly summarizes the characteristics of the insects in this order and the type of transmission mechanisms for viral and bacterial plant pathogens. RNAi products developed for other insects are also analyzed. Emphasis was made on the need for innovative management approaches to offset the threat of resistance by both the insect vector to insecticides and the pathogens to microbicides. Subsequently, the RNAi technology is described, which is particularly an ingenious method currently utilized in itself or in combination with other modern biotechnological innovations for managing important vector insects that could provide an additional powerful tool for use in integrated pest control programs.The requirements and recent advances for performing RNAi assays are detailed and an overview is given on how to produce cheaper double-stranded RNA as the main component of RNAi-based biopesticide. Examples of agricultural companies that use RNAi biotechnology in their product development were also discussed.

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Something old, something new, something borrowed, something red: the origin of ecologically relevant novelties in Hemiptera

Cited by 8 publications

References 64 publications

Genome editing of thevermilionlocus generates a visible eye color marker forOncopeltus fasciatus

Genome editing of thevermilionlocus generates a visible eye color marker forOncopeltus fasciatus

Genome editing of the vermilion locus generates a visible eye color marker for Oncopeltus fasciatus

Disruption of transmission of plant pathogens in the insect order Hemiptera using recent advances in RNA interference biotechnology

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