Some Volatile Metabolites Produced by the Antifungal-Trichoderma Asperellum UZ-A4 Micromycete

Abstract

The fungus of the genus Trichoderma is characterized by high biological activity and the ability to synthesize many compounds of great scientific interest. Among them, producers of amino acids, nucleic and organic acids, vitamins, surface-active substances, numerous hydrolases, various antibiotics and other substances were revealed. In this work there were studied volatile organic compounds produced by the fungus Trichoderma asperellum Uz-A4, isolated from the soil of the cotton field of the Bukhara region of Uzbekistan infected with phytopathogens. The antagonistic activity of the fungus about phytopathogens Alternaria alternata, Aspergillus niger and Fusarium solani was evaluated. Trichoderma asperellum Uz-A4 micromyzet showed high activity on the 4 th day against Alternaria alternata and Fusarium solani about Aspergillus niger on the 9th day. From the culture fluid of the fungus Trichoderma asperellum Uz-A4, 11 main substances have been isolated and identified. Mass - spectrometric analysis has shown that these are the substances of Phenylethylcohol; 5-hydroxymethylfurfural; Dehydroa Ceticacid; 1-Dodecanol; 2,4-di-tert-Butylphenol; Diethyl Suberate; n-hexadecanoic acid; 1-hexadecanol, 2-methyl; Phthalic Acid, Ethyl Pentadecyl Ester; Mono (2-Thylhexyl) Phthalate; Octadecanoic Acid. A chemical formula, molecular weight and the absorption spectrum of these substances have been determined. The results indicated that these secondary metabolites could be useful for biological control applications of T. asperellum Uz-A4 strain against diverse plant pathogens.