Some universal media for the isolation, growth and purity checking of a broad spectrum of microorganisms

Ka, Malik

doi:10.1007/bf01198765

Cited by 7 publications

(3 citation statements)

References 5 publications

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“…AYM agar medium, comprising yeast extract, mannitol, agar‐agar, and essential nutrients, were prepared and autoclaved for sterilization. The nodule tissues were inoculated onto AYM agar plates, and following an incubation period, individual bacterial colonies were developed (Malik, 1992 ), using Congo red , whereby only rhizobia were morphologically identified from other microorganisms.…”

Section: Methodsmentioning

confidence: 99%

Determination of abundance and symbiotic effectiveness of native rhizobia nodulating soybean and other legumes in Rwanda

Nzeyimana,

Onwonga,

Ayuke

et al. 2024

Plant Enviro Interactions

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Rhizobia diversity in the rhizosphere is one of the key promoters of biological nitrogen fixation between host legumes and microsymbionts, although related complex interaction may depend on various factors. This research was intended to assess the abundance of indigenous rhizobia isolates under various soil conditions, as well as their effectiveness to nodulate legumes such as soybeans. Factors such as soil properties and legume species influence the volume and symbiotic effectiveness of native rhizobia to nodulate crop legumes. To investigate the abundance of rhizobia isolates, legume crops were uprooted to obtain nodules for most probable number (MPN) determination of rhizobia isolates, and soybean (Glycine max.) was used to verify the presence of suitable and efficient rhizobia strains for nitrogen fixation. Soil samples were obtained from the holes out of which nodules were collected, and the laboratory analysis included pH, Mg, K, available P, organic C, Ca, and N to establish the correlation between the soil status and number of rhizobia isolates' cells. Significant variations (p‐value <.05) were observed in the cell counts of Rhizobia isolates from Glycine max, Phaseolus vulgaris, Pisum sativum, and Vigna unguiculata, particularly when compared to Arachis hypogaea isolates under acidic conditions. Notably, Pisum sativum and Vigna unguiculata showed consistent performance across all pH conditions. The number of rhizobia isolates was found to be significantly linked to total N and P deficiencies (p < .05). It was also established that total N was dependent on the number of rhizobia cells and that there is a strong correlation between organic carbon and N content. This study highlights the crucial role of understanding and optimizing conditions for rhizobia nodulation in diverse soil environments, emphasizing its potential impact on enhancing biological nitrogen fixation in legumes.

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Section: Methodsmentioning

confidence: 99%

Determination of abundance and symbiotic effectiveness of native rhizobia nodulating soybean and other legumes in Rwanda

Nzeyimana,

Onwonga,

Ayuke

et al. 2024

Plant Enviro Interactions

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“…Cance, 1968). Strains were conserved by regular subcultures under sterile mineral oil and refrigeration (5°C-10°C), as described by Malik (1992).…”

Section: Methodsmentioning

confidence: 99%

<i>BACILLUS</i> SPECIES IN THE OCEANIC WATERS ADJACENT TO CUBA: ASSOCIATION BETWEEN THEIR DISTRIBUTION AND METABOLIC ACTIVITY

Lugioyo¹,

Coto

Álvarez

et al. 2010

Rev. Mar. Cost.

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The aim of the present work was to identify spore-forming Gram-positive Bacillus strains isolated from oceanic waters adjacent to Cuba and to establish a possible relationship between their distribution and the metabolic activity of the isolates. Total protein patterns, derived from SDS-PAGE, were used to build a non-rooted dendrogram where the strains appeared clustered in three nodes. No direct relationship was observed between a node and particular species. In contrast, according to the physical and chemical characteristics of the zones, node I was different from node II and III, since it comprises strains from the farthest zones of the coast, poorer in nutrients. On the other hand, nodes II and III mainly collect strains isolated from nutrient and organic matter-enriched zones. Associating the node clustering with metabolic activities, it was found that in node I the ratio: number of positive activities/strain was 2.3, followed by node II with a ratio of 3.3, and finally node III exhibiting a ratio equal to 3.7. This could suggest that different total protein patterns in bacteria belonging to the same specie, but coming from environments with different degree of nutrient richness, could be an indicator of the capacities of these microorganisms to adapt and live in different environments. El objetivo del presente trabajo fue la identificación de cepas de bacilos Gram-positivos esporulados aislados de las aguas oceánicas adyacentes a Cuba y el establecimiento de la posible relación entre la distribución y las actividades metabólicas de los aislados. A partir del patrón de proteínas obtenido mediante electroforesis SDS-PAGE, se construyó un dendrograma no enraizado, lo que permitió la agrupación de las cepas en tres nodos. No se observó una relación directa entre un nodo y especies particulares, sin embargo, se encontraron diferencias entre los nodos al considerar las características físicas y químicas de las zonas; el nodo I fue diferente a los nodos II y III. En ese nodo se agruparon bacterias aisladas de zonas alejadas de la costa, pobres en nutrientes. Por otro lado, los nodos II y III agrupan principalmente a cepas aisladas de aguas más enriquecidas en nutrientes y materia orgánica. Con respecto a la asociación de los aislados en los nodos con las actividades metabólicas, se encontró que la razón entre el número de actividades positivas y el número de aislados fue de 2.3 en el nodo I, seguida del nodo II con 3.3 y por último el nodo III con 3.7. Estos resultados sugieren que, los patrones diferentes de proteínas totales en bacterias que pertenecen a la misma especie, pero que provienen de ambientes con diferente grado de riqueza en nutrientes, podrían ser un indicador de las capacidades de estos microorganismos para adaptarse y vivir en diferentes ambientes.

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“…A pre-culture was prepared by inoculating a single colony in 10 ml of peptone medium consisting of (gl -1 ) peptone 30, yeast extract 10 and NaCl 5 (pH 7.0) and inoculated on a rotary shaker (240 rpm) for 24 h. The numbers of aerobic, heterotrophic, chemolithoautotrophic bacteria were determined on nutrient agar media for the isolation, growth and purification of microorganisms (Malik, 1992). Selective media with (NH4)2SO4 or Na NO2 were used in order to determine the most probable number of nitrite and nitrate bacteria respectively and peptonated water was used for ammonifying bacteria (Pochon, 1954;Gerhardt et al, 1994).…”

Section: Cultivation Mediamentioning

confidence: 99%

Degradation of organic wastes and recycling of nutrients enhanced by microbes in subterranean habitat

Prabhavathi¹

2012

Afr. J. Microbiol. Res.

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Degradation of organic wastes and recycling of nutrients enhanced by various microbes of both bacterial and fungal species available at the entrance, twilight and dark zones of four different caves such as Pannian, Samanar, Ushman and KKB caves at Madurai was investigated. The roles of chemoheterotrophic and chemoautotrophic bacteria were found to be essential in cave ecosystem. The most important chemoheterotrophs were ammonifiers such as Bacillus sp., Clostridium sp., Serratia sp. and Pseudomonas sp. Whereas Nitrite bacteria like Nitrosomonas sp., Nitrosococcus sp. and Nitrobacter winogradskyi, Nitrococcus mobilis derived energy from the oxidation of ammonia and nitrite respectively. Many heterotrophs thrive on the organic releases from nitrifying bacteria. Such heterotrophs involved in the nitrogen cycle were nitrate-reducing bacteria while denitrifiers were sparse in comparison to nitrate reducers due to absence of an anaerobic atmosphere. A total of 15 mesophilous and four thermophilous fungi were isolated from the cave soil samples. Among 15 mesophilous fungi, 11 species belong to Deuteromycetes, 3 species are zygomycetes and 1 species belongs to Ascomycetes. The number of species and CFU of thermophilous fungi also decreased towards dark zone. Serratia sp. and Bacillus sp. were isolated from all the samples at the E, T and D zones of the studied caves. These two bacterial colonies were subjected to quantify enzymes such as cellulose and amylase respectively. The production of cellulose by Serratia sp. at 35°C, pH 6.5 and in substrate glucose is considerably more. The production of amylase by Bacillus sp. seem to be more at 35°C and the substrate ammonium nitrate is considerably ideal for the synthesis of higher quantity of amylase by Bacillus sp. In all the caves, D zone samples had more cellulase and amylase enzymes than the E and T zone samples. Apart from cellulase and amylase, the presence of lipase, catalase and RuBisCo also were identified from the study caves. The nutrient NO 3 was found to be more in all the samples. In the caves, nitrogen (N) compound was more than P and K. The FT-IR spectrum results showed that stretching of mineralized compounds were more at the D zone samples compared with E and T zone samples, due to microbial role at D zone with the aid of their enzymes which eventually degrade the complex organic matter into simpler forms.

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Some universal media for the isolation, growth and purity checking of a broad spectrum of microorganisms

Cited by 7 publications

References 5 publications

Determination of abundance and symbiotic effectiveness of native rhizobia nodulating soybean and other legumes in Rwanda

Determination of abundance and symbiotic effectiveness of native rhizobia nodulating soybean and other legumes in Rwanda

<i>BACILLUS</i> SPECIES IN THE OCEANIC WATERS ADJACENT TO CUBA: ASSOCIATION BETWEEN THEIR DISTRIBUTION AND METABOLIC ACTIVITY

Degradation of organic wastes and recycling of nutrients enhanced by microbes in subterranean habitat

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