Some structural features of the peptide profile of myelin basic protein-hydrolyzing antibodies in schizophrenic patients

Zavialova, Maria G.; Parshukova, D.; Kazieva, L.Sh.; Skvortsov, V. S.; Smirnova, L.

doi:10.7717/peerj.15584

Cited by 3 publications

(3 citation statements)

References 64 publications

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“…However, this activity may have significant immunological effects in vivo, given the long half-life of antibodies and high titers of circulating antibodies [37][38][39], and the amount of viral proteins relative to antibodies is relatively low. In addition, a low activity level is also characteristic of other proteolytically active antibodies [40][41][42][43].…”

Section: Analysis Of the Catalytic Activity Of Antibodies In The Hydr...mentioning

confidence: 99%

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Natural Antibodies Produced in Vaccinated Patients and COVID-19 Convalescents Hydrolyze Recombinant RBD and Nucleocapsid (N) Proteins

Timofeeva,

Shayakhmetova,

Nikitin

et al. 2024

Biomedicines

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Antibodies are protein molecules whose primary function is to recognize antigens. However, recent studies have demonstrated their ability to hydrolyze specific substrates, such as proteins, oligopeptides, and nucleic acids. In 2023, two separate teams of researchers demonstrated the proteolytic activity of natural plasma antibodies from COVID-19 convalescents. These antibodies were found to hydrolyze the S-protein and corresponding oligopeptides. Our study shows that for antibodies with affinity to recombinant structural proteins of the SARS-CoV-2: S-protein, its fragment RBD and N-protein can only hydrolyze the corresponding protein substrates and are not cross-reactive. By using strict criteria, we have confirmed that this proteolytic activity is an intrinsic property of antibodies and is not caused by impurities co-eluting with them. This discovery suggests that natural proteolytic antibodies that hydrolyze proteins of the SARS-CoV-2 virus may have a positive impact on disease pathogenesis. It is also possible for these antibodies to work in combination with other antibodies that bind specific epitopes to enhance the process of virus neutralization.

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Section: Analysis Of the Catalytic Activity Of Antibodies In The Hydr...mentioning

confidence: 99%

“…Figure 3A- proteins relative to antibodies is relatively low. In addition, a low activity level is also characteristic of other proteolytically active antibodies [40][41][42][43].…”

Section: Electrophoretic Homogeneity Of Igg Preparationsmentioning

confidence: 99%

Natural Antibodies Produced in Vaccinated Patients and COVID-19 Convalescents Hydrolyze Recombinant RBD and Nucleocapsid (N) Proteins

Timofeeva,

Shayakhmetova,

Nikitin

et al. 2024

Biomedicines

View full text Add to dashboard Cite

show abstract

“…Moreover, the complexity of enzyme inhibition extends further into the field of schizophrenia research with investigations into antibodies targeting myelin basic protein (MBP) [ 2 ]. This study unravels the cryptic behavior of these antibodies and their proteolytic tendencies towards MBP.…”

mentioning

confidence: 99%

New Sight: Enzymes as Targets for Drug Development

Kim

2023

CIMB

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Blood Plasma Markers in Depressed Mice under Chronic Social Defeat Stress

Smagin,

Bezryadnov,

Zavialova

et al. 2024

Biomedicines

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It has previously been shown that, in mice, chronic social defeat stress in daily agonistic interactions leads to a depression-like state similar to that in depressive patients. With this model, it has become obvious that it is possible to study peripheral markers of the depression-like state in an experiment. This paper was aimed at searching for protein markers in the blood plasma of depressed mice in the chronic social conflict model, which allows for us to obtain male mice with repeated experiences of defeat. Proteomic analysis of blood plasma samples was conducted to identify proteins differentially expressed in this state. There were changes in the expression levels of the amyloid proteins SAA1, SAA4, and SAMP and apolipoproteins APOC3, APOD, and ADIPO in the blood plasma of depressed mice compared with controls (unstressed mice). Changes in the expression of serine protease inhibitors and/or proteins associated with lipid metabolism, inflammation, or immune function [ITIH4, SPA3, A1AT5, HTP (HP), CO9, and A2MG] were also found. Here, we showed that chronic social stress is accompanied by increased levels of amyloid proteins and apolipoproteins in blood plasma. A similarity was noted between the marker protein expression changes in the depressed mice and those in patients with Alzheimer’s disease. These data indicate a psychopathogenic role of chronic social stress, which can form a predisposition to neurodegenerative and/or psychoemotional disorders.

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Some structural features of the peptide profile of myelin basic protein-hydrolyzing antibodies in schizophrenic patients

Cited by 3 publications

References 64 publications

Natural Antibodies Produced in Vaccinated Patients and COVID-19 Convalescents Hydrolyze Recombinant RBD and Nucleocapsid (N) Proteins

Natural Antibodies Produced in Vaccinated Patients and COVID-19 Convalescents Hydrolyze Recombinant RBD and Nucleocapsid (N) Proteins

New Sight: Enzymes as Targets for Drug Development

Blood Plasma Markers in Depressed Mice under Chronic Social Defeat Stress

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