The NDVI was used to discriminate tobacco variety, assess fertilizer levels, and determine the impact of planting date on separating crops. A split plot design with four planting dates, September, October, November, and December, as main plots, variety as subplot, and fertilizer treatments as sub-subplots was used. Radiometric measurements were taken from 5 m × 5 m sampling plots, using a multispectral radiometer. The September, October, and November crops had significant variety x fertilizer treatment differences ( < 0.05) from the age of 10 weeks. T 66 and KRK26 varieties had similar ( > 0.05) NDVI values and these were greater ( < 0.5) than those for K E1. The 100% and the 150% fertilizer treatments were similar ( > 0.05) and both were greater ( < 0.05) than the 50% fertilizer treatments. All of the fertilizer and variety treatments at the December planting dates had similar reflectance characteristics ( > 0.05), which were lower ( < 0.05) than the September and October planting dates. The results showed that planting dates, varieties, and fertilizer levels could be distinguished using spectral data. Weeks 10-11 and 15 after the start of the experiment were optimal for separating the planting date effect.