Some sources of error in measuring regional blood flow with radioactive microspheres.

Buckberg, Gerald D.; Luck, Jerry C.; Payne, D. B.; Hoffman, Julien I.E.; Archie, Joseph P.; Fixler, David E

doi:10.1152/jappl.1971.31.4.598

Cited by 1,107 publications

(441 citation statements)

References 6 publications

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“…With microspheres, the statistical problems introduced by cutting the pieces smaller and smaller occur simply by virtue of the inability to represent relative flow quantitatively by a smaller and smaller integer number of spheres with some statistical probability of being deposited in proportion to flow. 39 In the limit, a very small piece of tissue either does or does not contain a sphere, which can scarcely convey graded information. The technique developed by Yipintsoi et al merits special consideration for use as a standard for estimating heterogeneity.…”

Section: Effects Of Heterogeneity Of Flow On Washoutmentioning

confidence: 99%

Physiology and theory of tracer washout techniques for the estimation of myocardial blood flow: Flow estimation from tracer washout

Bassingthwaighte

1977

Progress in Cardiovascular Diseases

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Section: Effects Of Heterogeneity Of Flow On Washoutmentioning

confidence: 99%

Physiology and theory of tracer washout techniques for the estimation of myocardial blood flow: Flow estimation from tracer washout

Bassingthwaighte

1977

Progress in Cardiovascular Diseases

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“…This is a substantial limitation, since the full characterization of the remodeling after a myocardial infarction requires follow-up studies at different time points. In addition, the spatial resolution of microspheres examinations is limited, since a minimum number of microspheres is required in tissue specimens to obtain a sufficient accuracy (6). The cited method using ultrasonic flow probes provides no spatial resolution.…”

mentioning

confidence: 99%

In vivo assessment of absolute perfusion and intracapillary blood volume in the murine myocardium by spin labeling magnetic resonance imaging

Streif¹,

Nahrendorf²,

Hiller³

et al. 2005

Magnetic Resonance in Med

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The absolute perfusion and the intracapillary or regional blood volume (RBV) in murine myocardium were assessed in vivo by spin labeling magnetic resonance imaging. Pixel-based perfusion and RBV maps were calculated at a pixel resolution of 469 ؋ 469 m and a slice thickness of 2 mm. The T 1 imaging module was a segmented inversion recovery snapshot fast low angle shot sequence with velocity compensation in all three gradient directions. The group average myocardial perfusion at baseline was determined to be 701 ؎ 53 mL (100 g ⅐ min) ؊1 for anesthesia with isoflurane (N ‫؍‬ 11) at a mean heart rate (HR) of 455 ؎ 10 beats per minute (bpm). This value is in good agreement with perfusion values determined by invasive microspheres examinations. For i.v. administration of the anesthetic Propofol, the baseline perfusion decreased to 383 ؎ 40 mL (100 g ⅐ min) ؊1 (N ‫؍‬ 17, P < 0.05 versus. isoflurane) at a mean heart rate of 261 ؎ 13 bpm (P < 0.05 versus isoflurane). In addition, six mice with myocardial infarction were studied under isoflurane anesthesia (HR 397 ؎ 7 bpm). The perfusion maps showed a clear decrease of the perfusion in the infarcted area. The perfusion in the remote myocardium decreased significantly to 476 ؎ 81 mL (100 g ⅐ min) ؊1 (P < 0.05 versus sham). Regarding the regional blood volume, a mean value of 11.8 ؎ 0.8 vol % was determined for healthy murine myocardium under anesthesia with Propofol (N ‫؍‬ 4, HR 233 ؎ 17 bpm). In total, the presented techniques provide noninvasive in vivo assessment of the perfusion and the regional blood volume in the murine myocardium for the first time and seem to be promising tools for the characterization of mouse models in cardiovascular research. Over the past decade, the mouse animal model has become an essential part of medical basic research. This is due to the high degree of similarity between the mouse and the human genomes of about 97% (1) and the resulting high relevance of mouse animal studies for human basic research. Regarding cardiovascular research, magnetic resonance imaging (MRI) provides noninvasive and accurate assessment of cardiac structure and function (2). Since the viability and the capability of the myocardium are strongly influenced by its microcirculation, a full characterization of the myocardium requires a quantification of parameters such as the perfusion and the intracapillary or regional blood volume (RBV). In particular, perfusion is of paramount importance as a physiologic parameter, since it strongly determines the function of organs and the severity of many diseases.Heart diseases involving ventricular dysfunction and hypertrophy show significant alterations of the myocardial perfusion and transgenic mouse models may be well suited to elucidate the underlying fundamental mechanisms.For the assessment of cardiac perfusion in mice, only a few methods have been presented to date. One possible approach is the quantification of the coronary flow in the isolated mouse heart by ultrasonic flow probes (3). The injection of labeled microsphe...

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“…Because it is based on the measurement of a finite number of microspheres, the precision of the measurement is dependent on the number of microspheres per sample (6). At Ͼ400 microspheres in a given sample, the flow determined will be with 95% confidence within 10% of the true flow.…”

mentioning

confidence: 99%

High-resolution imaging reveals a limit in spatial resolution of blood flow measurements by microspheres

Decking

Pai²,

Bennett³

et al. 2004

American Journal of Physiology-Heart and Circulatory Physiology

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Some sources of error in measuring regional blood flow with radioactive microspheres.

Cited by 1,107 publications

References 6 publications

Physiology and theory of tracer washout techniques for the estimation of myocardial blood flow: Flow estimation from tracer washout

Physiology and theory of tracer washout techniques for the estimation of myocardial blood flow: Flow estimation from tracer washout

In vivo assessment of absolute perfusion and intracapillary blood volume in the murine myocardium by spin labeling magnetic resonance imaging

High-resolution imaging reveals a limit in spatial resolution of blood flow measurements by microspheres

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