Some Results of Study on Counts and Morphology of Rumen Ciliate Protozoa in Pasture-Raised Mongolian Sheep Lamb

Purevtsogt, D; Zolzaya, M; Demberel, Shirchin; Dugersuren, Jamyan

doi:10.5564/mjas.v19i3.730

Mong. J. Agric. Sci.

2017

DOI: 10.5564/mjas.v19i3.730

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Some Results of Study on Counts and Morphology of Rumen Ciliate Protozoa in Pasture-Raised Mongolian Sheep Lamb

D Purevtsogt¹,

M Zolzaya²,

Shirchin Demberel³

et al.

Abstract: The present study was performed to investigate rumen protozoa (ciliates) in pasture-raised Mongolian sheep lambs with their aging, identify the genus and species using the manual relied on morphology of detected ciliates and determine their counts. As a result of this study, ciliates of 6 genera belonging to ordersEntodiniomorрhа and Holotrichа were detected and identified and they were aslo documented by photography. Ciliates are distinguished with their own specific features a nd their main characteristic is… Show more

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Do Ruminal Ciliates Select Their Preys and Prokaryotic Symbionts?

Park

2018

Front. Microbiol.

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Ruminal ciliates both preys on and form symbiotic relationships with other members of the ruminal microbiota for their survival. However, it remains elusive if they have selectivity over their preys or symbionts. In the present study, we investigated the above selectivity by identifying and comparing the free-living prokaryotes (FLP) and the ciliate-associated prokaryotes (CAP) using Illumina MiSeq sequencing of 16S rRNA gene amplicons. We used single ciliates cells of both monocultures of Entodinium caudatum and Epidinium caudatum and eight different ciliate genera isolated from fresh rumen fluid of dairy cows. Irrespective of the source (laboratory monocultures vs. fresh isolates) of the single ciliate cells, the CAP significantly differed from the FLP in microbiota community profiles. Many bacterial taxa were either enriched or almost exclusively found in the CAP across most of the ciliate genera. A number of bacteria were also found for the first time as ruminal bacteria in the CAP. However, no clear difference was found in methanogens between the CAP and the FLP, which was confirmed using methanogen-specific qPCR. These results suggest that ruminal ciliates probably select their preys and symbionts, the latter of which has rarely been found among the free-living ruminal prokaryotes. The bacteria enriched or exclusively found in the CAP can be target bacteria to detect and localize using specific probes designed from their 16S rRNA sequences, to characterize using single-cell genomics, or to isolate using new media designed based on genomic information.

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Do Ruminal Ciliates Select Their Preys and Prokaryotic Symbionts?

Park

2018

Front. Microbiol.

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Some Results of Study on Counts and Morphology of Rumen Ciliate Protozoa in Pasture-Raised Mongolian Sheep Lamb

Cited by 1 publication

References 1 publication

Do Ruminal Ciliates Select Their Preys and Prokaryotic Symbionts?

Do Ruminal Ciliates Select Their Preys and Prokaryotic Symbionts?

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