Some recent advances in thin-layer chromatography

Pataki, György

doi:10.1007/bf02255770

Cited by 23 publications

(1 citation statement)

References 51 publications

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“…Seven chromogenic reagents for detecting primary, secondary, and tertiary amines were systematically evaluated (24). Detection and documentation methods for polyamide TLC were reviewed (484)• Instrumental methods which have been employed for characterization and identification include: direct spectrophotometry and fluorometry for amino acids, peptides, and nucleic acids (328); mass spectrometry (363) for phenol and aniline derivatives (202); fluorescence and phosphorescence for steroids after treatment with sulfuric acid (75); and differential thermal analysis for dicarboxylic acids (274)• Free fatty acids have been identified by their spot shapes (105). Separated spots were eluted from the support into KBr powder on the TLC plate with 100-150 µ of a dry, nonpolar solvent; the KBr powder was added to a KBr microdisk and pelletized for infrared spectroscopy (84).…”

Section: Thin-layer Chromatographymentioning

confidence: 99%

Chromatography

Zweig¹,

Moore²,

Sherma³

1970

Anal. Chem.

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Section: Thin-layer Chromatographymentioning

confidence: 99%

Chromatography

Zweig¹,

Moore²,

Sherma³

1970

Anal. Chem.

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Direkte quantitative Photometrie an Dünnschicht-Chromatogrammen

Hezel

1973

Angew. Chem.

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Direct Quantitative Photometry on Thin Layer Chromatograms

Hezel

1973

Angew. Chem. Int. Ed. Engl.

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The principal methods for the direct photometric evaluation of thin layer chromatograms are discussed with the aid of examples. Since light is strongly scattered by TLC adsorbents, absorption measurements in reflection are preferable to transmission measurements. In the reflection measurements, an approximation method for the Kubelka-Munk function leads to satisfactory straight calibration lines. The working range for quantitative reflection measurements is in the pg range, and the detection limit is of the order of lo-". The standard deviation for spots of equal concentration is less than f 1%. The signal-to-background ratio is improved by simultaneous measurement of reflection and transmission. The measurement of diminution in fluorescence ("fluorescence quenching") is found to be less sensitive than the reflection at the absorption maximum. However, combined measurement of the reflection and the diminution of fluorescence gives an increase in sensitivity as compared to pure reflection measurements. As in solution photometry, fluorescence measurements are generally much more sensitive than absorption measurements. The working range is normally in the ng region, and the detection limit is around lo-' ' g. All the investigations described in this paper were carried out on the Zeiss chromatogram spectrophotometer.

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Some recent advances in thin-layer chromatography

Cited by 23 publications

References 51 publications

Chromatography

Chromatography

Direkte quantitative Photometrie an Dünnschicht-Chromatogrammen

Direct Quantitative Photometry on Thin Layer Chromatograms

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