Some properties of unglucosylated phage T2 DNA

The nature of the – transition of DNA in solution

“…--As can be seen from fig.1 B, --values, at which the B-A transition is observed in a DNA fibers (films)-water vapour system, studied by X-ray analysis [l- 3,6] and by optical methods [8,20,21]. Thus, Brahms and Pilet [20,21] give 0.95 and 0.75 relative humidity values for the limits of the --B-A transition region (regardless of the source of DNA), with most of their curves lying within 0.85 0.75.…”

“…families of forms [4]. To the3 family, along with the canonic B form belong the C [ 51, T and some other closely related forms [6] of DNA. A-DNA and the complementary RNA helices (A-l 1 and A-12) belong to the x family of conformations * To whom all correspondence should be sent: Dr V. 1.…”

“…38 [7]. No discrete conformation within the families seem to exist in solution and a continuous transition due to a gradual change in the helix parameters is possible [4], whereas the interfamily transition both in fibrous samples [6] and in solution [4] occurs abruptly.…”

Template Properties of Glucose-Deficient T-Even Bacteriophage DNA

“…A simple explanation would be that the binding of RNA polymerase to DNA is interfered with by the glucose moieties. Alternatively, the differences in template activities might result from the fact that glucose-deficient T-even DNA is more susceptible to some nucleases (17,20). That is, the glucose-deficient DNA might be a better template for RNA synthesis because it has received more nicks from nucleases present in the infected cell extracts from which it was isolated or present in the extracts used for in vitro RNA synthesis.…”

Computer analysis of conformational possibilities of double‐helical DNA

“…X-ray analysis has revealed the existence of several forms of the double-helical nucleic acid: A, B, C [1 ], T [2] and D [3]. It is therefore important to obtain a complete description of all possible conformations of the complementary helix.…”