Some Properties of two Strains of Watermelon Mosaic Virus

Bhargava, Bharti

doi:10.1111/j.1439-0434.1977.tb03969.x

Cited by 7 publications

(3 citation statements)

References 9 publications

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“…Seed transmission of WMV has not been detected by others using different isolates of WMV and other cucurbit hosts (Anderson, 1951b;Grogan et al , 1959;Greber, 1969;Thomas, 1971a;Fischer and Lockhart, 1974; Bhargava, 1977).…”

Section: Transmission and General Characteristicsmentioning

confidence: 95%

“…(197^) used modifications of Milne and Grogan's purification scheme using n-butanol clarification to purify WMV. Bakker (1971) and Bhargava (1977) used chloroform clarification to purify isolates of WMV. Fischer and Lockhart (1974) modified the procedure of Damirdagh and Shepherd (1970) to partially purify a Moroccan isolate of WMV.…”

Section: Differential Systemic and Local Lesion Hosts For Wmv-1 And Wmentioning

confidence: 99%

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Purification, partial characterization, and serology of the capsid and cylindrical inclusion proteins of four isolates of watermelon mosaic virus / by Robert H. Baum.

Baum¹

1980

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WMV-1 and WMV-2 Defined Antisera specific for the cylindrical inclusions of WMV-M, WMV-1, and WMV-2 in SDS immunodiffusion cests with sap and purified inclusions were obtained. Antisera to WMV-2 inclusions reacted heterologously with BCMV, dasheen mosaic virus (DMV) , soybean mosaic virus (SoyMV) and lettuce mosaic virus (LMV). WMV-M inclusion antiserum reacted heterologously with WMV-1, WMV-2, DMV, and PRSV and with some isolates of LMV. The WMV-1 inclusion antiserum did not react with either WMV-M or WMV-2 inclusions. Analysis of peptide fragments after cleavage of capsid proteins with cyanogen bromide (CNBr) showed distinct patterns for WMV-1, WMV-2, and WMV-M. Staphylococcus aureus V-8 protease digests of the cylindrical inclusions of WMV-1, WMV-2, and WMV-M were analyzed by disc polyacrylamide gel electrophoresis. While there were some similarities between patterns, significant differences existed. This study provides additional evidence that WMV-1 and WMV-2 are distinct viruses on the basis of inclusion serology and analysis of the peptides formed by partial cleavage of capsid and cylindrical inclusion proteins. Evidence is also provided that WMV-M is a distinct virus.

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Section: Transmission and General Characteristicsmentioning

confidence: 95%

Section: Differential Systemic and Local Lesion Hosts For Wmv-1 And Wmentioning

confidence: 99%

Purification, partial characterization, and serology of the capsid and cylindrical inclusion proteins of four isolates of watermelon mosaic virus / by Robert H. Baum.

Baum¹

1980

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“…In India, the occurrence of Begomovirus on different cucurbitaceous crops has been reported by several workers (Muniyappa et al 2003;Sohrab et al 2003;Rajinimala et al 2005;Rajinimala and Rabindran 2007;Singh et al 2008;Raj et al 2010;Tiwari et al 2010b). Trichosanthes dioica, being a cash crop of small land holding farmers in India, has not received much attention to virus elimination (Bhargava 1977;Raj et al 2011).…”

Section: Introductionmentioning

confidence: 95%

Detection and elimination ofBegomovirusinfectingTrichosanthes dioica(pointed gourd) plants in Uttar Pradesh, India

Tiwari

Rao

Khan

et al. 2012

Archives Of Phytopathology And Plant Protection

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Begomovirus is widely spread on cultivated crops in India. In a survey, infected plants of Trichosanthes dioica (Pointed gourd) with the symptoms of mosaic and leaf curling were collected and checked for Begomovirus infection through PCR analysis. Application of meristem culture technique for regeneration of virus free plants of T. dioica was assessed. Plantlets were regenerated from infected plants of T. dioica through meristem culture. Regenerated plants were found Begomovirus free as evident by PCR analysis suggesting the suitability of meristem culture technique for elimination of Begomovirus from infected plants of T. dioica. This is the first report on elimination of Begomovirus in T. dioica in India.

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